[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-456441-105":59,"doc-detail-456441-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","evaluation-of-urinary-extracellular-vesicles-and-micrornas-to-diagnose-urogenital-tuberculosis","Evaluation of urinary extracellular vesicles and microRNAs to diagnose urogenital tuberculosis","","India faces the highest tuberculosis burden worldwide, with extra-pulmonary disease observed in up to 45% of cases; urogenital tuberculosis (UGTB) accounts for about 20%. Because non-sputum diagnostic tools remain limited, microRNAs are investigated as biomarker candidates. This study evaluates urinary extracellular vesicles as a non-invasive source of microRNAs for adults to support UGTB diagnosis, focusing on diagnostic potential and measurable biomarker performance.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/evaluation-of-urinary-extracellular-vesicles-and-micrornas-to-diagnose-urogenital-tuberculosis/456441/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/evaluation-of-urinary-extracellular-vesicles-and-micrornas-to-diagnose-urogenital-tuberculosis/456441.png","ImageObject",300,407,{"name":92,"@type":93},"SANS","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-30","2026-09-30",true,{"@type":102,"interactionType":103,"userInteractionCount":81},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What diagnostic problem does the study address for urogenital tuberculosis?","Question",{"text":112,"@type":113},"The study addresses the lack of non-sputum diagnostic tools for urogenital tuberculosis and explores urinary extracellular vesicles microRNAs as an alternative biomarker source.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"Which microRNAs were evaluated as potential biomarkers?",{"text":117,"@type":113},"The study evaluates miRNA-155-5p, miRNA-26a-5p, and miRNA-29a-3p in urinary extracellular vesicles for diagnosing urogenital tuberculosis in adults.",{"name":119,"@type":110,"acceptedAnswer":120},"How were urinary extracellular vesicles characterized and microRNAs measured?",{"text":121,"@type":113},"Urinary extracellular vesicles were characterized using nanoparticle tracking analysis and flow cytometry, and urinary uEV microRNAs were quantified using qRT-PCR with U6 normalization.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},456441,1792186911,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":81,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":145,"preview_page_count":4},962090760505,"https://ap-avatar.wpscdn.com/davatar_155a257f0dc6eb9ab79c44ca47cae57d","| W | J | E | M | World Journal of\u003Cbr>Experimental Medicine |\n| --- | --- | --- | --- | --- |\n\nSubmit a Manuscript: [https://www.f6publishing.com](https://www.f6publishing.com) World J Exp Med 2025 September 20; 15(3): 105208  \nDOI: 10.5493/wjem.v15.i3.105208 ISSN 2220-315x (online)  \nORIGINAL ARTICLE  \nCase Control Study  \nEvaluation of urinary extracellular vesicles and microRNAs to diagnose urogenital tuberculosis  \nParijat Das, Dharmendra K Chaudhary, Richa Mishra, Swasti Tiwari  \nSpecialty type: Medicine, research and experimental  \nProvenance and peer review:  \nInvited article; Externally peer reviewed.  \nPeer-review model: Single blind  \nPeer-review report’s classification Scientific Quality: Grade A, Grade C, Grade C  \nNovelty: Grade A, Grade A, Grade B  \nCreativity or Innovation: Grade A, Grade B, Grade B  \nScientific Significance: Grade B, Grade B, Grade C  \nP-Reviewer: Liang JJ; Luo HC  \nReceived: January 15, 2025  \nRevised: April 22, 2025  \nAccepted: June 20, 2025  \nPublished online: September 20, 2025  \nProcessing time: 209 Days and 23.4 Hours  \nParijat Das, Dharmendra K Chaudhary, Department of Molecular Medicine and Biotechnology, Sanjay Gandhi Post-Graduate Institute of Medical Sciences, Lucknow 226014, Uttar Pradesh, India  \nRicha Mishra, Department of Microbiology, Sanjay Gandhi Postgrad Institute Medical Sciences, Lucknow 226014, Uttar Pradesh, India  \nSwasti Tiwari, Department of Molecular Medicine and Biotechnology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow 226014, Uttar Pradesh, India  \nCorresponding author: Swasti Tiwari, PhD, Professor, Department of Molecular Medicine and Biotechnology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Raebareli Road, Mawaiya, Lucknow 226014, Uttar Pradesh, [India.](India. tiwari.pgi@gmail.com)[ tiwari.pgi@gmail.com](India. tiwari.pgi@gmail.com)  \nAbstract  \nBACKGROUND  \nIndia has the highest tuberculosis (TB) burden in the world. Of the estimated annual 10 million TB cases, features of extra pulmonary TB are evident in up to 45% . Urogenital TB (UGTB) accounts for approximately 20% of those cases. The lack of non-sputum based diagnostic tools continue to hinder efforts to reduce the burden of UGTB. MicroRNAs (miRNAs) play a crucial role in biological pathways and can be used as a potential biomarker for TB. We evaluated urinary extracellular vesicles (uEVs) as non-invasive source to explore miRNAs with biomarker potential for UGTB.  \nAIM  \nTo evaluate the potential of miRNA-155-5p, miRNA-26a-5p and miRNA-29a-3p in uEVs to diagnose UGTB in adults.  \nMETHODS  \nuEV characterization was done using nanoparticle tracking analysis and flow cytometry. Quantitative reverse transcriptase polymerase chain reaction (qRTPCR) for urinary uEV-miRNAs were carried out in samples from patients with suspected UGTB, or Urinary tract infections [UTI, disease controls (DC)] and healthy controls (HCs) (n = 20/group) . U6 was used to normalize the qRT-PCR data. Receivers operating characteristic curves was used to calculate the diagnostic accuracy of uEV-miRNAs to differentiate UGTB from controls (DC and HCs) .  \n WJEM  [https://www.wjgnet.com](https://www.wjgnet.com) 1 September 20, 2025  Volume 15  Issue 3   \nDas P et al. The uEV miRNAs to diagnose UGTB  \nRESULTS  \nuEVs from UGTB or UTI patients had higher mean size, and also lower proportion of CD63 positive vesicles as compared to HC’s uEVs. Between UTI and UGTB, the mean size of uEVs was significantly higher in UTI cases. qRT-PCR analysis revealed a significantly lower abundance of miRNA-155-5p and miRNA-26a-5p in uEVs from UGTB relative to UTI (P value = 0.004) and HC (P value = 0.009) respectively n = 20/group). While, miRNA-29a-3p was higher in abundance in both UGTB and HCs’ uEV, relative to uEVs from UTI cases (P values = 0.004 and 0.002 respectively, n = 20/group) . Moreover, miRNA-155-5p [area under curve (AUC) = 0.88, P ≤ 0.0001] and miRNA- 29a-3p (AUC = 0.76, P value = 0.005) had optimal diagnost","cbCaihd2yP15uEiq","https://ap.wps.com/l/cbCaihd2yP15uEiq","pdf",2466059,13,"English","# Abstract\n## Background\n## Aim\n## Methods\n## Results\n## Conclusion\n## Core Tip","[{\"question\":\"What diagnostic problem does the study address for urogenital tuberculosis?\",\"answer\":\"The study addresses the lack of non-sputum diagnostic tools for urogenital tuberculosis and explores urinary extracellular vesicles microRNAs as an alternative biomarker source.\"},{\"question\":\"Which microRNAs were evaluated as potential biomarkers?\",\"answer\":\"The study evaluates miRNA-155-5p, miRNA-26a-5p, and miRNA-29a-3p in urinary extracellular vesicles for diagnosing urogenital tuberculosis in adults.\"},{\"question\":\"How were urinary extracellular vesicles characterized and microRNAs measured?\",\"answer\":\"Urinary extracellular vesicles were characterized using nanoparticle tracking analysis and flow cytometry, and urinary uEV microRNAs were quantified using qRT-PCR with U6 normalization.\"}]","Evaluation of urinary extracellular vesicles and microRNAs to diagnose urogenital tuberculosis | PDF",1790746039,33]