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Prostate cases showed significant miRNA expression changes, including increased miR-122-5p and reduced miR-125-5p, miR-16-5p.",{"@graph":14,"@context":73},[15,34,56],{"@type":16,"itemListElement":17},"BreadcrumbList",[18,23,27,31],{"item":19,"name":20,"@type":21,"position":22},"https://docshare.wps.com","Home","ListItem",1,{"item":24,"name":25,"@type":21,"position":26},"https://docshare.wps.com/document/","Document",2,{"item":28,"name":29,"@type":21,"position":30},"https://docshare.wps.com/document/healthcare/","Healthcare",3,{"item":32,"name":10,"@type":21,"position":33},"https://docshare.wps.com/document/evaluating-the-urinary-exosome-microrna-profile-in-prostate-cancer/363935/",4,{"url":32,"name":10,"@type":35,"image":36,"author":41,"headline":10,"publisher":44,"fileFormat":47,"inLanguage":8,"description":12,"dateModified":48,"datePublished":49,"encodingFormat":47,"isAccessibleForFree":50,"interactionStatistic":51},"DigitalDocument",{"url":37,"@type":38,"width":39,"height":40},"https://docshare.wps.com/thumbnails/evaluating-the-urinary-exosome-microrna-profile-in-prostate-cancer/363935.png","ImageObject",300,407,{"name":42,"@type":43},"awa","Person",{"url":19,"name":45,"@type":46},"DocShare","Organization","application/pdf","2026-10-27","2026-09-23",true,{"@type":52,"interactionType":53,"userInteractionCount":55},"InteractionCounter",{"@type":54},"ViewAction",11,{"@type":57,"mainEntity":58},"FAQPage",[59,65,69],{"name":60,"@type":61,"acceptedAnswer":62},"What biomarker did the study evaluate for prostate cancer?","Question",{"text":63,"@type":64},"The study evaluated urine-derived exosomal microRNA (miRNA) profiles as liquid biopsy biomarkers for prostate cancer.","Answer",{"name":66,"@type":61,"acceptedAnswer":67},"How were the urine samples and exosomes processed?",{"text":68,"@type":64},"Urine was collected from biopsy-proven prostate cancer patients, exosomes were extracted, and exosomal miRNAs were profiled using transcriptomic analysis.",{"name":70,"@type":61,"acceptedAnswer":71},"What key miRNA expression changes were reported in prostate cancer patients?",{"text":72,"@type":64},"Prostate cancer patients showed significantly increased miR-122-5p and decreased miR-125-5p and miR-16-5p in urine-derived exosomes.","https://schema.org",{"og:url":32,"og:type":75,"og:title":10,"og:site_name":45,"og:description":12},"article",{"robots":77,"canonical":32},"index,follow",{"doc_id":79,"site_id":7},363935,1790653892,{"code":4,"msg":82,"data":83},"success",[84,88,92,96,101,106,110,115,120,123,127],{"id":22,"doc_module":4,"doc_module_name":25,"category_name":85,"show_sort_weight":86,"slug":87},"Story & Novel",90,"story-novel",{"id":26,"doc_module":4,"doc_module_name":25,"category_name":89,"show_sort_weight":90,"slug":91},"Literature",80,"literature",{"id":33,"doc_module":4,"doc_module_name":25,"category_name":93,"show_sort_weight":94,"slug":95},"Exam",70,"exam",{"id":97,"doc_module":4,"doc_module_name":25,"category_name":98,"show_sort_weight":99,"slug":100},5,"Comic",60,"comic",{"id":102,"doc_module":4,"doc_module_name":25,"category_name":103,"show_sort_weight":104,"slug":105},6,"Technology",50,"technology",{"id":107,"doc_module":4,"doc_module_name":25,"category_name":29,"show_sort_weight":108,"slug":109},7,40,"healthcare",{"id":111,"doc_module":4,"doc_module_name":25,"category_name":112,"show_sort_weight":113,"slug":114},8,"Research & Report",30,"research-report",{"id":116,"doc_module":4,"doc_module_name":25,"category_name":117,"show_sort_weight":118,"slug":119},9,"Religion & Spirituality",20,"religion-spirituality",{"id":118,"doc_module":4,"doc_module_name":25,"category_name":121,"show_sort_weight":118,"slug":122},"World Cup","world-cup",{"id":124,"doc_module":4,"doc_module_name":25,"category_name":125,"show_sort_weight":124,"slug":126},10,"Lifestyle","lifestyle",{"id":128,"doc_module":4,"doc_module_name":25,"category_name":129,"show_sort_weight":97,"slug":130},19,"General","general",{"code":4,"msg":82,"data":132},{"doc_id":79,"user_id":133,"nickname":42,"user_avatar":134,"doc_module":4,"category_id":107,"category_name":29,"doc_title":10,"doc_description":12,"doc_content":135,"file_id":136,"file_url":137,"file_type":138,"file_size":139,"view_count":55,"is_deleted":4,"is_public":22,"is_downloadable":22,"audit_status":22,"page_count":140,"language":141,"language_code":8,"site_id":7,"html_lang":8,"table_of_contents":142,"faqs":143,"seo_title":144,"seo_description":12,"update_tm":145,"read_time":146},3985747858093,"https://ap-avatar.wpscdn.com/davatar_3d24733baf745e90a7e4bdd5f77d97b2","Article  \nEvaluating the Urinary Exosome MicroRNA Profile in Prostate Cancer  \nBeatriz Walter Rodriguez 1, Christopher J. Ricketts 2, Baris Turkbey 3, Peter A. Pinto 2 and Maria J. Merino 1, *  \nAcademic Editor: GuorongLi  \nReceived: 12 June 2026  \nRevised: 9 July 2026  \nAccepted: 10 July 2026  \nPublished: 15 July 2026  \nCopyright: © 2026 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.  \n1 Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA; [beatriz.walterrodriguez@nih.gov](beatriz.walterrodriguez@nih.gov)  \n2 Urologic Oncology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA; [chris.ricketts@nih.gov](chris.ricketts@nih.gov) (C.J.R.); [pintop@mail.nih.gov](pintop@mail.nih.gov) (P.A.P.)  \n3 Molecular Imaging Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA; [ismail.turkbey@nih.gov](ismail.turkbey@nih.gov)  \n* [Correspondence: mjmerino@mail.nih.gov](Correspondence: mjmerino@mail.nih.gov); Tel.: +1-301-480-8483  \nAbstract  \nBackground/Objectives: Prostate cancer is the second most frequent cancer among men and the 5th leading cause of cancer death among men worldwide. Identification of urinederived biomarkers, such as exosomal miRNAs, in liquid biopsies for prostate cancer could be very beneficial for screening and active surveillance. Methods: Urine was collected from 42 patients with biopsy-proven evidence of prostate cancer and exosomes were extracted. Transcriptomic analysis was performed on the urine-derived exosomal miRNA and compared to the urine-derived exosomal miRNA profiles from 10 normal control donorsand 15 von Hippel-Lindau (VHL) syndrome patients with clear cell renal cell carcinoma (ccRCC). Results: Urine-derived exosomal miRNA profiles of prostate patients were significantly different from normal control individuals. Significantly increased expression of miR-122-5p and decreased expression of miR-125-5p and miR-16-5p were observed in the urine-derived exosomes from prostate cancer patients. Significant upregulation of miR- 30a-5p and downregulation of miR-320-5p, miR-320b, and miR-320c were observed in the urine-derived exosomes from both prostate cancer patients and VHL patients with ccRCC, indicating these miRNAs could be non-specific markers of urological cancer. Increased expression of miR-10a-5p and miR-30e-5p or miR-532-5p and miR-206 correlated with the presence of either extracapsular or perineural invasion, respectively. Conclusions: This study highlights the potential for urine-derived exosomal miRNA profiles to identify the presence of prostate cancer and predict clinical features, additionally showing that miRNA signals could be non-specific markers of urologic cancer types. Further validation studies are necessary to demonstrate the utility of urine-derived exosomal miRNA profiles as biomarkers for diagnosis or prognosis in prostate cancer.  \nKeywords: prostate cancer; urinary exosomes; miRNA; Gleason score; liquid biopsy; molecular markers  \n1. Introduction  \nProstate cancer (PC) is the second most frequent cancer among men and the 5th leading cause of cancer death among men worldwide [1] . The American Cancer Society estimated that in the United States 313,780 new cases will be diagnosed in 2025, with approximately 35,770 individuals dying from prostate cancer ([https://cancerstatisticscenter.cancer.org/](https://cancerstatisticscenter.cancer.org/)[ ](https://cancerstatisticscenter.cancer.org/)[types/prostate](types/prostate) ([accessed on 4 August 2025](accessed on 4 August 2025))) . Although the number of prostate cancer cases  \ndiagnosed had been declining, since 2014 the incidence rate has been increasing by ~3% per year overall [2] . Simila","cbCaihkUgINLmiD0","https://ap.wps.com/l/cbCaihkUgINLmiD0","pdf",3165507,14,"English","# Introduction\n## Liquid biopsy and urine-based biomarkers\n# Methods\n## Patient cohorts and exosome isolation\n## miRNA transcriptomic profiling\n# Results\n## Differential miRNA expression in prostate cancer\n## Associations with invasion features\n# Conclusions\n## Diagnostic and prognostic potential","[{\"question\":\"What biomarker did the study evaluate for prostate cancer?\",\"answer\":\"The study evaluated urine-derived exosomal microRNA (miRNA) profiles as liquid biopsy biomarkers for prostate cancer.\"},{\"question\":\"How were the urine samples and exosomes processed?\",\"answer\":\"Urine was collected from biopsy-proven prostate cancer patients, exosomes were extracted, and exosomal miRNAs were profiled using transcriptomic analysis.\"},{\"question\":\"What key miRNA expression changes were reported in prostate cancer patients?\",\"answer\":\"Prostate cancer patients showed significantly increased miR-122-5p and decreased miR-125-5p and miR-16-5p in urine-derived exosomes.\"}]","Evaluating the Urinary Exosome MicroRNA Profile in Prostate Cancer | PDF",1790154288,35]