[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"doc-seo-148550-105":3,"detail-sidebar-cat-0-en-105":81,"doc-detail-148550-en":131},{"code":4,"msg":5,"data":6},0,"ok",{"site_id":7,"language":8,"slug":9,"title":10,"keywords":11,"description":12,"schema_data":13,"social_meta":74,"head_meta":76,"extra_data":78,"updated_unix":80},105,"en","eurasian-journal-of-veterinary-sciences-the-evaluation-of-biofilm-production-by-streptococcus-equi-zooepidemicus-isolates-research-article","Eurasian Journal of Veterinary Sciences - The evaluation of biofilm production by Streptococcus equi zooepidemicus isolates - Research Article","","This research evaluates the biofilm production capacity of Streptococcus equi zooepidemicus isolates from equine samples. Forty one strains obtained from the laboratory stock collection of Selçuk University Veterinary Faculty were assessed using Congo red agar, crystal violet staining, and scanning electron microscopy. Results showed incompatibility between Congo red agar and crystal violet staining. Biofilm formation was confirmed by SEM through visualization of biofilm layers, supporting the ability of these isolates to form biofilm under in vitro conditions.",{"@graph":14,"@context":73},[15,34,56],{"@type":16,"itemListElement":17},"BreadcrumbList",[18,23,27,31],{"item":19,"name":20,"@type":21,"position":22},"https://docshare.wps.com","Home","ListItem",1,{"item":24,"name":25,"@type":21,"position":26},"https://docshare.wps.com/document/","Document",2,{"item":28,"name":29,"@type":21,"position":30},"https://docshare.wps.com/document/research-report/","Research & Report",3,{"item":32,"name":10,"@type":21,"position":33},"https://docshare.wps.com/document/eurasian-journal-of-veterinary-sciences-the-evaluation-of-biofilm-production-by-streptococcus-equi-zooepidemicus-isolates-research-article/148550/",4,{"url":32,"name":10,"@type":35,"image":36,"author":41,"headline":10,"publisher":44,"fileFormat":47,"inLanguage":8,"description":12,"dateModified":48,"datePublished":49,"encodingFormat":47,"isAccessibleForFree":50,"interactionStatistic":51},"DigitalDocument",{"url":37,"@type":38,"width":39,"height":40},"https://docshare.wps.com/thumbnails/eurasian-journal-of-veterinary-sciences-the-evaluation-of-biofilm-production-by-streptococcus-equi-zooepidemicus-isolates-research-article/148550.png","ImageObject",300,407,{"name":42,"@type":43},"Emma Wilson","Person",{"url":19,"name":45,"@type":46},"DocShare","Organization","application/pdf","2026-10-29","2026-08-26",true,{"@type":52,"interactionType":53,"userInteractionCount":55},"InteractionCounter",{"@type":54},"ViewAction",12,{"@type":57,"mainEntity":58},"FAQPage",[59,65,69],{"name":60,"@type":61,"acceptedAnswer":62},"What was the main objective of this study?","Question",{"text":63,"@type":64},"To measure the biofilm production capabilities of Streptococcus equi zooepidemicus isolates.","Answer",{"name":66,"@type":61,"acceptedAnswer":67},"Which methods were used to evaluate biofilm production?",{"text":68,"@type":64},"Congo red agar, crystal violet staining, and scanning electron microscopy (SEM) were used to assess biofilm formation.",{"name":70,"@type":61,"acceptedAnswer":71},"Why are the findings important for veterinary clinicians?",{"text":72,"@type":64},"The study indicates that equine clinicians should be aware that these isolates can form biofilm in vitro, which is relevant to infection persistence and treatment difficulty.","https://schema.org",{"og:url":32,"og:type":75,"og:title":10,"og:site_name":45,"og:description":12},"article",{"robots":77,"canonical":32},"index,follow",{"doc_id":79,"site_id":7},148550,1787781160,{"code":4,"msg":82,"data":83},"success",[84,88,92,96,101,106,111,115,120,123,127],{"id":22,"doc_module":4,"doc_module_name":25,"category_name":85,"show_sort_weight":86,"slug":87},"Story & Novel",90,"story-novel",{"id":26,"doc_module":4,"doc_module_name":25,"category_name":89,"show_sort_weight":90,"slug":91},"Literature",80,"literature",{"id":33,"doc_module":4,"doc_module_name":25,"category_name":93,"show_sort_weight":94,"slug":95},"Exam",70,"exam",{"id":97,"doc_module":4,"doc_module_name":25,"category_name":98,"show_sort_weight":99,"slug":100},5,"Comic",60,"comic",{"id":102,"doc_module":4,"doc_module_name":25,"category_name":103,"show_sort_weight":104,"slug":105},6,"Technology",50,"technology",{"id":107,"doc_module":4,"doc_module_name":25,"category_name":108,"show_sort_weight":109,"slug":110},7,"Healthcare",40,"healthcare",{"id":112,"doc_module":4,"doc_module_name":25,"category_name":29,"show_sort_weight":113,"slug":114},8,30,"research-report",{"id":116,"doc_module":4,"doc_module_name":25,"category_name":117,"show_sort_weight":118,"slug":119},9,"Religion & Spirituality",20,"religion-spirituality",{"id":118,"doc_module":4,"doc_module_name":25,"category_name":121,"show_sort_weight":118,"slug":122},"World Cup","world-cup",{"id":124,"doc_module":4,"doc_module_name":25,"category_name":125,"show_sort_weight":124,"slug":126},10,"Lifestyle","lifestyle",{"id":128,"doc_module":4,"doc_module_name":25,"category_name":129,"show_sort_weight":97,"slug":130},19,"General","general",{"code":4,"msg":82,"data":132},{"doc_id":79,"user_id":133,"nickname":42,"user_avatar":134,"doc_module":4,"category_id":112,"category_name":29,"doc_title":10,"doc_description":12,"doc_content":135,"file_id":136,"file_url":137,"file_type":138,"file_size":139,"view_count":55,"is_deleted":4,"is_public":22,"is_downloadable":22,"audit_status":22,"page_count":97,"language":140,"language_code":8,"site_id":7,"html_lang":8,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":12,"update_tm":80,"read_time":144,"preview_page_count":4},3848291630094,"https://eur-avatar.wpscdn.com/davatar_085a072bc5b1113ac321206ff7593b45","Eurasian Journal  \nof Veterinary Sciences  \n[www.eurasianjvetsci.org](www.eurasianjvetsci.org)  \nRESEARCH ARTICLE  \nThe evaluation of biofilm production by Streptococcus equi zooepidemicus isolates  \nBilal Osamah Mohammed Mohammed1, Uçkun Sait Uçan1*  \n1Selcuk University, Veterinary Faculty, Department of Microbiology, Konya, Turkey  \nReceived:12.03.2020, Accepted: 09.10.2020  \n*[usucan@selcuk.edu.tr](usucan@selcuk.edu.tr)  \nStreptococcus equi zooepidemicus izolatlarının biofilm üretiminin değerlendirilmesi  \nEurasian J Vet Sci, 2020, 36, 4, 312-316  \nDOI: 10.15312/EurasianJVetSci.2020.313  \nÖz  \nAmaç: Streptococcus equi zooepidemicus izolatlarının biofilm üretme kapasitelerinin ölçülmesi amaçlandı .  \nGereç ve Yöntem: Selçuk Üniversitesi Veteriner Fakültesi Mikrobiyoloji Anabilim Dalı kültür koleksiyonundan sağlanan kırk adet Streptococcus equi zooepidemicus izolatı Kongo kırmızısı agar, Kristal moru boyama ve taramalı electron mikroskobu (TEM) olmak üzere 3 farklı yöntem ile biofilm oluşturma yönünden değerlendirildi.  \nBulgular: Kongo kırmızısı agar ile Kristal moru boyama sonuçları arasında uyumsuzluk vardı . Otuz bir pozitif suşun 19’u Kongo kırmızısı agarda kuvvetli pozitif verirken 30’u Kristal moru boyama ilekuvvetli pozitif bulundu. Biofilm üretimi TEM ile doğrulandı .  \nÖneri: Ülkede at hekimliği hizmeti veren klinisyenlerin, Streptococcus equi zooepidemicus etkenlerinin in vitro şartlarda biofilm oluşturabileceğinin farkında olmaları önerilir.  \nAnahtar kelimeler: Streptococcus equi zooepidemicus, biofilm.  \nAbstract  \nAim: The purpose of this study is to measure biofim production capabilities of Streptococcus equi zooepidemicus isolates.  \nMaterials and Methods: Forty one horse strains of Streptococcus equi zooepidemicus from the laboratory stock collection of Microbiology Department of the Veterinary Faculty, Selçuk University were evaluated by the biofilm formation using three different methods: Congo red agar, crystal violet staining method and scanning electron microscopy (SEM) .  \nResults: Incompatibility between the results obtained from Congo red agar and crystal violet staining methods was detected. Nineteen out of 31 positive strains gave strong positive on Congo red agar while 30 of them were considered as strongly biofilm producing strains in crystal violet staining method. The biofilm production was confirmed and the biofilm layers were detected by using the SEM microscope.  \nConclusion: Equine clinicians in this Country should be aware of that Streptococcus equi zooepidemicus isolates are capable of forming biofilm in vitro.  \nKeywords: Streptococcus equi zooepidemicus, biofilm.  \n312  \nEurasian J Vet Sci, 2020, 36, 4, 312-316  \nBiofilm production by S. equi zooepidemicus Mohammed and Ucan  \nIntroduction  \nThe Lancefield group C member the Streptococcus equi subspecies zooepidemicus is a commensal organism of the tonsil and nasopharyngeal mucosa of the equine. This species may opportunistically start respiratory infections such as purulent rhinitis, bronchitis and pneumonia in foals and donkeys of different ages. In older horses that have been under heat or transportation stress, it may also cause acute hemorrhagic pneumonia (Sellon and Long 2013, Filioussis and Karavanis 2019) . In terms of veterinary public issues this agent needs to be focused since it may even be transmitted from dogs to human leading a severe disease as evidenced recently (Zahlanie et al 2019) .  \nBiofilm is a factor that may contribute to infections and disease development. Day by day facing infections related to biofilm is becoming a bigger problem. The negative impact of biofilm can be seen in health and industrial sector as well.  \nDuring infection the formation of biofilm layer prevents the contact of immune cells with the invading bacterial populations. It also helps the microorganisms to resist extreme heat, pH, UV and other environmental conditions. Inside the intestine, the biofilm formation on the epithelial surface prot","cbCaiebtYR0HDarc","https://ap.wps.com/l/cbCaiebtYR0HDarc","pdf",691303,"English","# Introduction\n## Biofilm and infection relevance\n## Biofilm formation mechanisms\n## Biofilm impact on antibiotics and treatment\n## Clinical significance in veterinary contexts\n# Materials and Methods\n## Isolate collection\n## Biofilm detection methods\n# Results\n## Congo red agar vs crystal violet discrepancy\n## SEM confirmation\n# Conclusion","[{\"question\":\"What was the main objective of this study?\",\"answer\":\"To measure the biofilm production capabilities of Streptococcus equi zooepidemicus isolates.\"},{\"question\":\"Which methods were used to evaluate biofilm production?\",\"answer\":\"Congo red agar, crystal violet staining, and scanning electron microscopy (SEM) were used to assess biofilm formation.\"},{\"question\":\"Why are the findings important for veterinary clinicians?\",\"answer\":\"The study indicates that equine clinicians should be aware that these isolates can form biofilm in vitro, which is relevant to infection persistence and treatment difficulty.\"}]","Eurasian Journal of Veterinary Sciences - The evaluation of biofilm production by Streptococcus equi zooepidemicus isolates - Research Article | PDF",13]