[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-381042-105":59,"doc-detail-381042-en":131},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":124,"head_meta":126,"extra_data":128,"updated_unix":130},105,"en","estrogen-associates-with-female-predominance-in-xp112-translocation-renal-cell-carcinoma","Estrogen associates with female predominance in Xp11.2 translocation renal cell carcinoma","","Estrogen associates with female predominance in Xp11.2 translocation renal cell carcinoma (Xp11.2 tRCC) through an epidemiology-based and mechanistic framework. The study reviewed 1005 Xp11.2 tRCC cases, showing a female predominance and an onset trend paralleling estrogen’s physiological rhythm. Estrogen-treated HK-2 cells underwent RNA-sequencing, and downstream analyses using TCGA and GEO indicated estrogen-related downregulation of Rad54l2, enrichment in DNA damage repair and cancer pathways, and supportive bioinformatic risk associations for clinical outcomes.",{"@graph":69,"@context":123},[70,84,106],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/estrogen-associates-with-female-predominance-in-xp112-translocation-renal-cell-carcinoma/381042/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/estrogen-associates-with-female-predominance-in-xp112-translocation-renal-cell-carcinoma/381042.png","ImageObject",300,407,{"name":92,"@type":93},"Ivy","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-11-03","2026-09-24",true,{"@type":102,"interactionType":103,"userInteractionCount":105},"InteractionCounter",{"@type":104},"ViewAction",11,{"@type":107,"mainEntity":108},"FAQPage",[109,115,119],{"name":110,"@type":111,"acceptedAnswer":112},"What epidemiological feature characterizes Xp11.2 translocation renal cell carcinoma in this study?","Question",{"text":113,"@type":114},"The study reports female predominance in Xp11.2 tRCC, with the onset trend in females resembling estrogen’s physiological rhythm.","Answer",{"name":116,"@type":111,"acceptedAnswer":117},"How was Rad54l2 linked to estrogen and Xp11.2 tRCC?",{"text":118,"@type":114},"After estrogen treatment in HK-2 cells and in Xp11.2 tRCC contexts, Rad54l2 was downregulated, and pathway enrichment analysis supported estrogen-associated changes in DNA damage repair and cancer-related clusters.",{"name":120,"@type":111,"acceptedAnswer":121},"What methods were used to investigate the molecular mechanisms?",{"text":122,"@type":114},"The study used RNA-sequencing in estrogen-treated HK-2 cells, followed by bioinformatic analyses based on TCGA and GEO data, and included a literature search using databases such as PubMed, MEDLINE, EMBASE, and others.","https://schema.org",{"og:url":83,"og:type":125,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":127,"canonical":83},"index,follow",{"doc_id":129,"site_id":62},381042,1790930428,{"code":4,"msg":5,"data":132},{"doc_id":129,"user_id":133,"nickname":92,"user_avatar":134,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":135,"file_id":136,"file_url":137,"file_type":138,"file_size":139,"view_count":105,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":140,"language":141,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":142,"faqs":143,"seo_title":144,"seo_description":67,"update_tm":145,"read_time":146},549758252649,"https://ap-avatar.wpscdn.com/avatar/8000253669c5317157?_k=1778319167496531819","[www. nature.com/scientificreports](www. nature.com/scientificreports)  \nOPEN  \nEstrogen associates with female predominance in Xp11.2 translocation renal cell carcinoma  \nYanwen Lu1, Yiqi Zhu1, Wenliang Ma1, Ning Liu1, Xiang Dong1, Qiancheng Shi1, Fei Yu2, Hongqian Guo1, Dongmei Li3,4 & Weidong Gan1*  \nBased on the epidemiological characteristics of susceptibility and age selectivity for women in Xp11.2 translocation renal cell carcinoma (Xp11.2 tRCC), we inferred that estrogen was to be blamed. Rad54 like 2 (Rad54l2) which might be one of key effector proteins of DNA damage mediated by estrogen was downregulated in numerous cancers, however, its role in epidemiological characteristics of Xp11.2 tRCC was needed to further study. We reviewed 1005 Xp11.2 tRCC cases and collected estrogen data and then compared the onset time of Xp11.2 tRCC cases in female with estrogen changing trend. An RNA-sequencing was performed in estrogen treated HK-2 cells and subsequently bioinformatic analysis was applied based on the Cancer Genome Atlas (TCGA) and GEO database. The male-tofemale ratio of Xp11.2 tRCC was 1:1.4 and the median age of onset was 29.7 years old. The onset trend of female was similar to estrogen physiological rhythm (r = 0.67, p \u003C 0.01) . In Xp11.2 tRCC and HK-2 cells after estrogen treatment, Rad54l2 was downregulated, and GSEA showed that pathways significantly enriched in DNA damage repair and cancer related clusters after estrogen treated, as well as GO and KEGG analysis. Downregulation of Rad54l2 was in numerous cancers, including renal cell carcinoma (RCC), in which Rad54l2 expression was significantly decreased in male, age over  \n60 years old, T2&T3&T4 stages, pathologic SII&SIII&SIV stages as well as histologic G3&G4 grades, and cox regression analysis proved that Rad54l2 expression was a risk factor for overall survival, disease-specific survival and progression-free interval in univariate analysis. There existed female predominance in Xp11.2 tRCC and Rad54l2 might play vital role in estrogen mediating female predominance in Xp11.2 tRCC.  \nXp11.2 translocation renal cell carcinoma (Xp11.2 tRCC) was firstly defined as a distinctive renal cell carcinoma (RCC) entity in 20041 and then reclassified into microphthalmia (MiT) family tRCC in 20162. This category of RCC is caused by breakage of the transcription factor enhancer 3 ( TFE3) gene on X-chromosome and then balanced translocation with partner genes, such as ASPL, PRCC, SFPQ, NonO and CLTC3–10.  \nXp11.2 tRCC was firstly reported in a two-year-old child 11. Recent literatures showed that Xp11.2 tRCC occurred mostly in young people12 and the median age of onset is 29 years old13. However, in non-Xp11.2 tRCC, the median age is in the sixth and seventh decades14. A meta-analysis had revealed that the incidence of female is higher than that of male15, which is similar to the previous report by Cheng et al.16. In contrast, large-scale epidemiological investigation show that RCC is more common in male regardless of pathological type with a male-to-female ratio of 2:1 17. Therefore, female predominance in childbearing period may be a noteworthy feature of Xp11.2 tRCC according to the age and gender data.  \nThe gender disparity in RCC had been shown to be associated with sex steroids18, 19. Preceding literature had also reported that estrogen could increase risk of topoisomerase IIβ-mediated TFE3 breaks to initiate Xp11.2 tRCC20. This epidemiological feature may suggest that estrogen is a risk factor of Xp11.2 tRCC.  \nMoreover, androgen and androgen receptor could facilitate Xp11.2 tRCC progression21. Estrogen influenced the DNA damage response (DDR) and DNA repair through the regulation of key effector proteins, such as ATR,  \n1Department of Urology, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, No. 321 Zhongshan Road, Nanjing 210008, Jiangsu, People’s Republic of China. 2Department of Laboratory Medicine, Nanjing Children’s Ho","cbCaia5gwsJBvpvo","https://ap.wps.com/l/cbCaia5gwsJBvpvo","pdf",7269211,14,"English","# Materials and methods\n## Literature search and study selection","[{\"question\":\"What epidemiological feature characterizes Xp11.2 translocation renal cell carcinoma in this study?\",\"answer\":\"The study reports female predominance in Xp11.2 tRCC, with the onset trend in females resembling estrogen’s physiological rhythm.\"},{\"question\":\"How was Rad54l2 linked to estrogen and Xp11.2 tRCC?\",\"answer\":\"After estrogen treatment in HK-2 cells and in Xp11.2 tRCC contexts, Rad54l2 was downregulated, and pathway enrichment analysis supported estrogen-associated changes in DNA damage repair and cancer-related clusters.\"},{\"question\":\"What methods were used to investigate the molecular mechanisms?\",\"answer\":\"The study used RNA-sequencing in estrogen-treated HK-2 cells, followed by bioinformatic analyses based on TCGA and GEO data, and included a literature search using databases such as PubMed, MEDLINE, EMBASE, and others.\"}]","Estrogen associates with female predominance in Xp11.2 translocation renal cell carcinoma | PDF",1790243279,35]