[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-337230-105":59,"doc-detail-337230-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","establishing-a-new-human-lung-squamous-cell-carcinoma-cell-line-omul-1-expressing-insulin-like-growth-factor-1-receptor-and-programmed-cell-death-ligand-1","Establishing a new human lung squamous cell carcinoma cell line, OMUL-1, expressing insulin-like growth factor 1 receptor and programmed cell death ligand 1","","Squamous cell carcinoma is the second most prevalent type of non–small cell lung cancer, and progress in molecular mechanism research requires reliable squamous lung cancer cell models. A novel lung squamous cell line, OMUL-1, was generated from primary tumor tissue of a 74-year-old man, and its growth behavior, tumorigenicity, histology, gene expression, and invasion were characterized using RT-PCR, RNA sequencing, and functional assays. OMUL-1 formed tumors in mice, showed squamous histology, expressed multiple receptor/kinase genes, and responded to an IGF1R inhibitor; IGF1R and PD-L1 were detected in primary and xenograft tumors.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/establishing-a-new-human-lung-squamous-cell-carcinoma-cell-line-omul-1-expressing-insulin-like-growth-factor-1-receptor-and-programmed-cell-death-ligand-1/337230/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/establishing-a-new-human-lung-squamous-cell-carcinoma-cell-line-omul-1-expressing-insulin-like-growth-factor-1-receptor-and-programmed-cell-death-ligand-1/337230.png","ImageObject",300,407,{"name":92,"@type":93},"Aurelia","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-30","2026-09-21",true,{"@type":102,"interactionType":103,"userInteractionCount":52},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"Why was a new squamous lung cancer cell line needed?","Question",{"text":112,"@type":113},"Squamous cell carcinoma is common and has poor outcomes, and studying molecular mechanisms requires useful squamous lung cancer cell lines as research tools. The document notes that only a limited number of squamous lung carcinoma cell lines exist worldwide.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How was OMUL-1 established and what patient information is described?",{"text":117,"@type":113},"OMUL-1 was derived from the primary lung cancer of a 74-year-old Japanese man with a long smoking history. Imaging and biopsy confirmed squamous cell carcinoma, and surgery including lower lobectomy and mediastinal lymph node dissection was performed.",{"name":119,"@type":110,"acceptedAnswer":120},"What key biological characteristics did OMUL-1 show?",{"text":121,"@type":113},"OMUL-1 formed tumors in mice with 100% subcutaneous tumor formation. Histology indicated squamous cell carcinoma, invasion ability was lower than other developed cell lines, and gene expression profiles showed multiple receptors/kinases including IGF1R; picropodophyllin inhibited OMUL-1 growth.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},337230,1790444226,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":52,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":44,"language":139,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":140,"faqs":141,"seo_title":142,"seo_description":67,"update_tm":143,"read_time":144},1099514068365,"https://ap-avatar.wpscdn.com/avatar/10000253d8d9f28188e?_k=1776742907772140068","DOI: 10.1111/1759-7714.15488  \nORIGINA L ARTICLE  \nEstablishing a new human lung squamous cell carcinoma cell line, OMUL-1, expressing insulin-like growth factor 1 receptor and programmed cell death ligand 1  \nHiroaki Nagamine 1  | Masakazu Yashiro 2 | Megumi Mizutani 1 |  \nAkira Sugimoto 1  | Yoshiya Matsumoto 1 | Yoko Tani 3 | Hiroyasu Kaneda 3 | Kazuhiro Yamada 1 | Tetsuya Watanabe 1 | Kazuhisa Asai 1 | Satoshi Suzuki 4 | Tomoya Kawaguchi 1,3  \n1Department of Respiratory Medicine, Graduate School of Medicine, Osaka Metropolitan University, Osaka, Japan  \n2Molecular Oncology and Therapeutics, Graduate School of Medicine, Osaka Metropolitan University, Osaka, Japan  \n3Department of Clinical Oncology, Graduate School of Medicine, Osaka Metropolitan University, Osaka, Japan  \n4Department of Thoracic Surgery, Graduate School of Medicine, Osaka Metropolitan University, Osaka, Japan  \nCorrespondence  \nMasakazu Yashiro, Molecular Oncology and Therapeutics, Osaka Metropolitan University, Graduate School of Medicine, 1-4-3 Asahimachi, Abeno-ku, Osaka 545-8585, Japan.  \nEmail: [i21496f@omu.ac.jp](i21496f@omu.ac.jp)  \nAbstract  \nThe Main Problem: Squamous cell carcinoma is the second most prevalent type of non–small cell lung cancer. Analyzing the molecular mechanisms underlying lung carcinoma requires useful tools, such as squamous lung cancer cell lines.  \nMethods: A novel new lung squamous cell carcinoma cell line, OMUL-1, was developed from the primary lung cancer of a 74-year-old man. We assessed the characteristics and behavior of OMUL-1 cells were examined, including their growth kinetics, tumorigenicity in mice, histological properties, gene expression profiles using reverse transcription polymerase chain reaction (RT-PCR), and RNA sequencing and invasion assays.  \nResults: OMUL-1-an adherent cell line-resulted in 100% tumor formation when subcutaneously injected into mice. Histological analysis of the subcutaneous tumor using hematoxylin and eosin staining revealed squamous cell carcinoma with characteristics similar to those of the primary tumor (p40 and p63 were positive, and TTF-1 was negative) . An invasion assay demonstrated that OMUL-1 had a lower invasion ability compared to that of other developed cell lines. RT-PCR analysis and RNA sequencing indicated that OMUL-1 cells expressed FGFR1, FGFR2, FGFR3, FGFR4, EGFR, HER2, ErbB3, ErbB4, VEGFR3, IGF1R, c-MET, PDGFRa, and PDGFRb. Additionally, picropodophyllin (an IGF1R inhibitor) significantly inhibited the growth of OMUL-1 cells. Immunohistochemistry revealed that IGF1R and PD-L1 were expressed in both the primary and subcutaneous tumors.  \nConclusions: We developed a novel new squamous cell lung carcinoma cell line, OMUL-1, that expresses IGF1R and PD-L1 .  \nKEYW ORD S  \ncell line, IGF1R, lung cancer, PD-L1, squamous cell carcinoma  \nINTRODUCTION  \nLung cancer is the primary cause of cancer-related deaths worldwide.1 Non–small cell lung cancer (NSCLC) accounts  \nfor 85% of all lung cancers. Squamous cell carcinoma is the second most prevalent type and accounts for 30% of all NSCLC cases, followed by adenocarcinoma.2 Owing to its high frequency and poor prognosis, understanding the  \nThis is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.  \n© 2024 The Author(s) . Thoracic Cancer published by John Wiley & Sons Australia, Ltd.  \nunderlying mechanisms of carcinogenesis, proliferation, and metastasis in lung squamous cell carcinoma and the development of novel treatment strategies are crucial. Various driver genes, such as epidermal growth factor receptor (EGFR)3 and anaplastic lymphoma kinase (ALK),4 have been identified in NSCLC. Additionally, their inhibitors are promising therapeutic agents of adenocarcinoma.5–8 However, molecularly targeted inhibit","cbCaioqqYVA505i6","https://ap.wps.com/l/cbCaioqqYVA505i6","pdf",10352303,"English","# Abstract\n## Background\n## Methods\n## Results\n## Conclusions\n# Introduction\n## Epidemiology and unmet needs\n## Role of PD-L1 and ICIs\n## Need for squamous cell line tools\n# Methods\n## Patient","[{\"question\":\"Why was a new squamous lung cancer cell line needed?\",\"answer\":\"Squamous cell carcinoma is common and has poor outcomes, and studying molecular mechanisms requires useful squamous lung cancer cell lines as research tools. The document notes that only a limited number of squamous lung carcinoma cell lines exist worldwide.\"},{\"question\":\"How was OMUL-1 established and what patient information is described?\",\"answer\":\"OMUL-1 was derived from the primary lung cancer of a 74-year-old Japanese man with a long smoking history. Imaging and biopsy confirmed squamous cell carcinoma, and surgery including lower lobectomy and mediastinal lymph node dissection was performed.\"},{\"question\":\"What key biological characteristics did OMUL-1 show?\",\"answer\":\"OMUL-1 formed tumors in mice with 100% subcutaneous tumor formation. Histology indicated squamous cell carcinoma, invasion ability was lower than other developed cell lines, and gene expression profiles showed multiple receptors/kinases including IGF1R; picropodophyllin inhibited OMUL-1 growth.\"}]","Establishing a new human lung squamous cell carcinoma cell line, OMUL-1, expressing insulin-like growth factor 1 receptor and programmed cell death ligand 1 | PDF",1790018225,23]