[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-384528-105":59,"doc-detail-384528-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","epigenetic-modification-of-gene-expression-in-cancer-cells-by-terahertz-demethylation","Epigenetic modification of gene expression in cancer cells by terahertz demethylation","","Terahertz (THz) radiation can modulate DNA methylation, an epigenetic regulator controlling gene expression. The study demonstrates resonant THz demethylation in living SK-MEL-3 melanoma cells, using an enzyme-linked immunosorbent assay to quantify global DNA methylation changes. A 1.6-THz, limited-bandwidth exposure caused 19% demethylation without significant apurinic/apyrimidinic site occurrence, and demethylation increased linearly with THz power. THz demethylation downregulates FOS, JUN, and CXCL8, implicating cancer and apoptosis pathways, supporting therapeutic potential as a gene-expression modifier.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/epigenetic-modification-of-gene-expression-in-cancer-cells-by-terahertz-demethylation/384528/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/epigenetic-modification-of-gene-expression-in-cancer-cells-by-terahertz-demethylation/384528.png","ImageObject",300,407,{"name":92,"@type":93},"Himbo","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-29","2026-09-24",true,{"@type":102,"interactionType":103,"userInteractionCount":39},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"How does terahertz (THz) demethylation affect gene expression in cancer cells?","Question",{"text":112,"@type":113},"Resonant THz radiation demethylates living melanoma cells, leading to measurable changes in global DNA methylation. It also downregulates cancer- and apoptosis-related genes including FOS, JUN, and CXCL8.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"What experimental evidence supports DNA demethylation after THz exposure?",{"text":117,"@type":113},"An enzyme-linked immunosorbent assay showed a 19% reduction in global DNA methylation in SK-MEL-3 cells under 1.6-THz irradiation with limited spectral bandwidth.",{"name":119,"@type":110,"acceptedAnswer":120},"Does THz demethylation cause significant DNA damage such as apurinic/apyrimidinic sites?",{"text":121,"@type":113},"The study reports no significant occurrence of apurinic/apyrimidinic sites following THz demethylation.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},384528,1790644477,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":39,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":41},687197100911,"https://ap-avatar.wpscdn.com/avatar/a000239b6f1da00475?x-image-process=image/resize,m_fixed,w_180,h_180&k=1785132997149421697","[www. nature.com/scientificreports](www. nature.com/scientificreports)  \nOPEN  \nEpigenetic modification of gene expression in cancer cells by terahertz demethylation  \nHwayeong Cheon1, Junho K. Hur2, Woochang Hwang3, Hee‑Jin Yang4* & Joo‑Hiuk Son5*  \nTerahertz (THz) radiation can affect the degree of DNA methylation, the spectral characteristics of which exist in the terahertz region. DNA methylation is an epigenetic modification in which a methyl (CH3) group is attached to cytosine, a nucleobase in human DNA. Appropriately controlled DNA methylation leads to proper regulation of gene expression. However, abnormal gene expression that departs from controlled genetic transcription through aberrant DNA methylation may occur in cancer or other diseases. In this study, we demonstrate the modification of gene expression in cells by THz demethylation using resonant THz radiation. Using an enzyme‑linked immunosorbent assay, we observed changes in the degree of global DNA methylation inthe SK‑MEL‑3 melanoma cell line under irradiation with 1.6‑THz radiation with limited spectral bandwidth. Resonant THz radiation demethylated living melanoma cells by 19%, with no significant occurrence of apurinic/apyrimidinic sites, and the demethylation ratio was linearly proportional to the power ofTHz radiation. THzdemethylation downregulates FOS, JUN, and CXCL8 genes, which are involved in cancer and apoptosis pathways. Our results show that THz demethylation has the potential to be a gene expression modifier with promising applications in cancer treatment.  \nDNA methylation is an epigenetic mechanism in which a methyl group (CH3−) is attached to the fifth carbon of the cytosine ring in DNA, thereby regulating gene expression1–3. Methylated cytosine modulates gene expression by inhibiting transcription4–7. In normal cells, DNA methylation acts as a gene switch. Most cytosinephosphate-guanine clusters (known as CpG islands) control gene expression by promoter methylation (activating expression) or demethylation (deactivating expression) . When the regulatory mechanism of gene expression is disordered due to aberrant DNA methylation, gene-related diseases may occur (Suppl. Fig. 1) . Inhibition of cancer suppressor genes by DNA methylation is known to be related to carcinogenesis8–10. Many studies have indicated an association between aberrant DNA methylation and alterations in gene expression in several cancer types, including melanoma, colon, lung, breast, ovary, bladder, liver, and cervical cancers11–22.  \nAberrant DNA methylation contributes to abnormal genetic phenotypes similar to genetic mutations in cancer. Although alteration of genome sequences is generally irreversible, the demethylation process may have the potential to reverse DNA methylation (by placing the appropriate methyl groups at the proper cytosine) . On May 19, 2004, VidazaTM (5-Azacytidine) became the first US FDA-approved methylation-inhibiting agent for the treatment of myelodysplastic syndrome (MDS)23,24. DecitabineTM (2′-dexoy-5-azacytidine) has also been approved by the US and EU for treatment of MDS and acute myeloid leukemia (AML)24,25. Demethylation drugs inhibit all isoforms of DNMT and induce various types of tumor suppressors. These dilution processes of DNA methylation depend on genomic heredity and are referred to as passive demethylation (Suppl. Fig. 4) .  \nIn contrast, active DNA demethylation refers to an enzymatic process that removes methyl groups directly from cytosine. The mechanism of active DNA methylation is not yet fully understood; however, almost all DNA regions are demethylated by the activation of enzymes during reprogramming. In particular, the ten–eleven translocation (TET) family of methylated cytosine dioxygenases is used independently for active demethylation26–28. Consequently, the activity of TET enzymes exchanges 5-mC for nonmethylated cytosine directly, completing  \n1Biomedical Engineering Research Center, Asan Medical Center, 88, Olympic-ro","cbCainCdMBT0Livx","https://ap.wps.com/l/cbCainCdMBT0Livx","pdf",2888133,12,"English","# Epigenetic modification and DNA methylation\n# THz radiation and demethylation mechanism\n# Experimental approach: global methylation changes\n# Gene regulation outcomes after THz demethylation\n# Therapeutic implications for cancer treatment","[{\"question\":\"How does terahertz (THz) demethylation affect gene expression in cancer cells?\",\"answer\":\"Resonant THz radiation demethylates living melanoma cells, leading to measurable changes in global DNA methylation. It also downregulates cancer- and apoptosis-related genes including FOS, JUN, and CXCL8.\"},{\"question\":\"What experimental evidence supports DNA demethylation after THz exposure?\",\"answer\":\"An enzyme-linked immunosorbent assay showed a 19% reduction in global DNA methylation in SK-MEL-3 cells under 1.6-THz irradiation with limited spectral bandwidth.\"},{\"question\":\"Does THz demethylation cause significant DNA damage such as apurinic/apyrimidinic sites?\",\"answer\":\"The study reports no significant occurrence of apurinic/apyrimidinic sites following THz demethylation.\"}]","Epigenetic modification of gene expression in cancer cells by terahertz demethylation | PDF",1790263916]