[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-detail-445909-en":59,"doc-seo-445909-105":81},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":5,"data":60},{"doc_id":61,"user_id":62,"nickname":63,"user_avatar":64,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":66,"doc_content":67,"file_id":68,"file_url":69,"file_type":70,"file_size":71,"view_count":29,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":72,"language":73,"language_code":74,"site_id":75,"html_lang":74,"table_of_contents":76,"faqs":77,"seo_title":78,"seo_description":66,"update_tm":79,"read_time":80,"preview_page_count":4},445909,2336474466412,"\tJames","https://ap-avatar.wpscdn.com/davatar_155a257f0dc6eb9ab79c44ca47cae57d","Enhancing Radiosensitivity of Boron Neutron Capture Therapy for Liver Cancer with Homologous Recombination Repair Inhibitor","Hepatocellular carcinoma, especially in recurrent or treatment-refractory settings, often responds poorly to radiation therapy, elevating the risk of radiation-induced liver disease and limiting therapeutic options. Boric acid-mediated boron neutron capture therapy (BA-BNCT) is explored as an approach to improve outcomes for radioresistant HCC. This study enhances BA-BNCT efficacy by targeting DNA homologous recombination repair, using a RAD51 inhibitor B02 to sensitize HepG2 and radioresistant HepG2R cells and to examine autophagy and apoptosis-related mechanisms after treatment.","Japanese Journal of Radiology (2026) 44:196–208 [https://doi.org/10.1007/s1](https://doi.org/10.1007/s1) 1604-025-01852-z  \nEnhancing radiosensitivity of boron neutron capture therapy for liver cancer with homologous recombination repair inhibitor  \nZih‑Yin Lai1,2,3 · Yu‑Hsuan Huang1,2,3 · Ting‑Yu Zhou1,2,3 · Chi‑Ying Lee1,2,3 · Yu‑Ming Shiao4 · Yi‑Wei Chen5 · Fong‑In Chou3 · Jen‑Kun Chen6 · Yung‑Jen Chuang1,2,3  \nReceived: 12 April 2025 / Accepted: 4 August 2025 / Published online: 18 August 2025 © The Author(s) 2025  \nAbstract  \nBackground Hepatocellular carcinoma (HCC), particularly in recurrent or treatment-refractory cases, often exhibits poor responsiveness to radiation therapy, increasing the risk of radiation-induced liver disease, necessitating innovative treatment approaches. Boric acid-mediated boron neutron capture therapy (BA-BNCT) has emerged as a promising approach for liver cancer. This study aims to improve BA-BNCT efficacy for radioresistant HCC by exploring sensitization agents, enhancing treatment while minimizing irradiation doses and side effects.  \nMethods We targeted the DNA homologous recombination repair (HRR) protein RAD51 . Before neutron irradiation, a RAD51 inhibitor, B02, was administered to evaluate its sensitization effect on both HepG2 and the radioresistant HepG2R cells. We examined the cell death mechanism, focusing on the expression profile of LC3B after BA-BNCT, to investigate its impact on DNA repair responses, especially on autophagy and apoptosis.  \nResults We observed that inhibition of RAD51 led to increased γH2AX, the DNA double-strand break marker. Additionally, combining the RAD51 inhibitor B02 with BA-BNCT resulted in tumor cell arrest in the G0/G 1 phase, indicating altered cell cycle regulation. In exploring cell death mechanisms, we observed increased autophagy following BNCT, potentially as a response to cellular stress induced by DNA damage in tumor cells. The combination of B02 and BA-BNCT significantly disrupted autophagic flux and promoted apoptosis in the tumor cells.  \nConclusions Combining a RAD51 inhibitor with BA-BNCT significantly enhances the anti-tumor efficacy against radioresistant HCC and parental HCC cells. This proof-of-concept study suggests that the combination treatment can achieve comparable or superior therapeutic effects using lower radiation doses, thereby reinforcing the potential of BNCT for treating recurrent HCC.  \nKeywords Hepatocellular Carcinoma · BNCT · RAD51 · Autophagy · DNA Repair  \nAbbreviations  \n7-AAD 7-Amino-Actinomycin D  \nBA Boric acid  \nBNCT Boron neutron capture therapy BPA Boronophenylalanine  \nZih-Yin Lai and Yu-Hsuan Huang contributed equally to this work.  \n* Jen-Kun Chen [jkchen@nhri.edu.tw](jkchen@nhri.edu.tw)  \n* Yung-Jen Chuang [yjchuang@life.nthu.edu.tw](yjchuang@life.nthu.edu.tw)  \n1 School of Medicine, National Tsing Hua University, Hsinchu, Taiwan R.O.C.  \n2 Institute of Bioinformatics and Structural Biology, National Tsing Hua University, Hsinchu, Taiwan R.O.C.  \nBRCA1 Breast cancer 1  \nBRCA2 Breast cancer 2  \nDDR DNA damage response  \nDSB DNA double-strand break  \nDSBR Double-strand break repair  \nEBRT External Beam Radiation Therapy GAPDH Glyceraldehyde 3-phosphate dehydrogenase  \n3 Nuclear Science and Technology Development Center, National Tsing Hua University, Hsinchu, Taiwan R.O.C.  \n4 GeneReach Biotechnology, Taichung, Taiwan R.O.C.  \n5 Department of Heavy Particles and Radiation Oncology, Taipei Veterans General Hospital, Taipei, Taiwan R.O.C.  \n6 Institute of Biomedical Engineering and Nanomedicine, National Health Research Institutes, Miaoli, Taiwan R.O.C.  \nGy Gray  \nHCC Hepatocellular carcinoma  \nHPA Human Protein Atlas  \nHR Homologous recombination  \nHRR Homologous recombination repair IC50 50% Growth-inhibitory concentration LAT1 L-type amino acid transporter 1  \nNGS Next-generation sequencing γH2AX Phosphorylated-H2AX RER Radiation enhancement ratio  \nRILD Radiation-induced liver disease  \nSER Sensitizer en","cbCaitXMReigvwp1","https://ap.wps.com/l/cbCaitXMReigvwp1","pdf",5159954,13,"English","en",105,"# Abstract\n## Background\n## Methods\n## Results\n## Conclusions\n# Keywords\n# Abbreviations\n# Introduction","[{\"question\":\"Why is improving radiosensitivity important in recurrent hepatocellular carcinoma?\",\"answer\":\"Recurrent or treatment-refractory HCC often shows poor radiation responsiveness, increasing the risk of radiation-induced liver disease and making effective radiation dosing difficult.\"},{\"question\":\"What is the main sensitization strategy used in this study?\",\"answer\":\"The study inhibits DNA homologous recombination repair by targeting RAD51, using the RAD51 inhibitor B02 before BA-BNCT to evaluate sensitization in HepG2 and HepG2R cells.\"},{\"question\":\"How does the combination of B02 with BA-BNCT affect cell death mechanisms?\",\"answer\":\"RAD51 inhibition increases the DNA double-strand break marker γH2AX. Combined treatment disrupts autophagic flux and promotes apoptosis, while also altering cell-cycle regulation with tumor arrest at the G0/G1 phase.\"}]","Enhancing Radiosensitivity of Boron Neutron Capture Therapy for Liver Cancer with Homologous Recombination Repair Inhibitor | PDF",1790713221,33,{"code":4,"msg":82,"data":83},"ok",{"site_id":75,"language":74,"slug":84,"title":65,"keywords":85,"description":66,"schema_data":86,"social_meta":140,"head_meta":142,"extra_data":144,"updated_unix":145},"enhancing-radiosensitivity-of-boron-neutron-capture-therapy-for-liver-cancer-with-homologous-recombination-repair-inhibitor","",{"@graph":87,"@context":139},[88,102,122],{"@type":89,"itemListElement":90},"BreadcrumbList",[91,95,97,100],{"item":92,"name":93,"@type":94,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":96,"name":9,"@type":94,"position":14},"https://docshare.wps.com/document/",{"item":98,"name":35,"@type":94,"position":99},"https://docshare.wps.com/document/healthcare/",3,{"item":101,"name":65,"@type":94,"position":19},"https://docshare.wps.com/document/enhancing-radiosensitivity-of-boron-neutron-capture-therapy-for-liver-cancer-with-homologous-recombination-repair-inhibitor/445909/",{"url":101,"name":65,"@type":103,"image":104,"author":109,"headline":65,"publisher":111,"fileFormat":114,"inLanguage":74,"description":66,"dateModified":115,"datePublished":116,"encodingFormat":114,"isAccessibleForFree":117,"interactionStatistic":118},"DigitalDocument",{"url":105,"@type":106,"width":107,"height":108},"https://docshare.wps.com/thumbnails/enhancing-radiosensitivity-of-boron-neutron-capture-therapy-for-liver-cancer-with-homologous-recombination-repair-inhibitor/445909.png","ImageObject",300,407,{"name":63,"@type":110},"Person",{"url":92,"name":112,"@type":113},"DocShare","Organization","application/pdf","2026-11-01","2026-09-29",true,{"@type":119,"interactionType":120,"userInteractionCount":29},"InteractionCounter",{"@type":121},"ViewAction",{"@type":123,"mainEntity":124},"FAQPage",[125,131,135],{"name":126,"@type":127,"acceptedAnswer":128},"Why is improving radiosensitivity important in recurrent hepatocellular carcinoma?","Question",{"text":129,"@type":130},"Recurrent or treatment-refractory HCC often shows poor radiation responsiveness, increasing the risk of radiation-induced liver disease and making effective radiation dosing difficult.","Answer",{"name":132,"@type":127,"acceptedAnswer":133},"What is the main sensitization strategy used in this study?",{"text":134,"@type":130},"The study inhibits DNA homologous recombination repair by targeting RAD51, using the RAD51 inhibitor B02 before BA-BNCT to evaluate sensitization in HepG2 and HepG2R cells.",{"name":136,"@type":127,"acceptedAnswer":137},"How does the combination of B02 with BA-BNCT affect cell death mechanisms?",{"text":138,"@type":130},"RAD51 inhibition increases the DNA double-strand break marker γH2AX. Combined treatment disrupts autophagic flux and promotes apoptosis, while also altering cell-cycle regulation with tumor arrest at the G0/G1 phase.","https://schema.org",{"og:url":101,"og:type":141,"og:title":65,"og:site_name":112,"og:description":66},"article",{"robots":143,"canonical":101},"index,follow",{"doc_id":61,"site_id":75},1790896754]