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This study examines how EF-hand domain containing 1 (EFHD1) expression relates to clinical features within the TME. Bioinformatic analyses evaluate EFHD1 mRNA relevance, followed by multiplex immunohistochemistry to assess EFHD1 protein in tumor and stromal compartments. EFHD1 overexpression links to invasion depth and TNM stage, associates with CD66b+ neutrophil infiltration, and independently predicts worse prognosis via Cox regression.",{"@graph":69,"@context":126},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/efhd1-expression-is-correlated-with-tumor-infiltrating-neutrophils-and-predicts-prognosis-in-gastric-cancer/378332/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/efhd1-expression-is-correlated-with-tumor-infiltrating-neutrophils-and-predicts-prognosis-in-gastric-cancer/378332.png","ImageObject",300,407,{"name":92,"@type":93},"Mabel","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-11-02","2026-09-24",true,{"@type":102,"interactionType":103,"userInteractionCount":24},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118,122],{"name":109,"@type":110,"acceptedAnswer":111},"What is the study aimed at determining in gastric cancer?","Question",{"text":112,"@type":113},"The study investigates whether EFHD1 expression is associated with the tumor microenvironment and its clinical significance in gastric cancer.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How do the authors assess EFHD1 in the tumor microenvironment?",{"text":117,"@type":113},"They use bioinformatic analyses for EFHD1 mRNA in gastric carcinoma tissues and multiplex immunohistochemistry to evaluate EFHD1 protein within TME compartments.",{"name":119,"@type":110,"acceptedAnswer":120},"What clinical relationships does EFHD1 expression show?",{"text":121,"@type":113},"EFHD1 protein presentation correlates with invasion depth and TNM stage and is significantly related to intratumoral and stromal CD66b+ neutrophil infiltration.",{"name":123,"@type":110,"acceptedAnswer":124},"Does EFHD1 have prognostic value for patients with gastric cancer?",{"text":125,"@type":113},"Yes. Cox regression analysis indicates EFHD1 is an independent prognostic predictor with a hazard ratio of 2.262 (P \u003C 0.001).","https://schema.org",{"og:url":83,"og:type":128,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":130,"canonical":83},"index,follow",{"doc_id":132,"site_id":62},378332,1790937859,{"code":4,"msg":5,"data":135},{"doc_id":132,"user_id":136,"nickname":92,"user_avatar":137,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":138,"file_id":139,"file_url":140,"file_type":141,"file_size":142,"view_count":24,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":39,"language":143,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":144,"faqs":145,"seo_title":146,"seo_description":67,"update_tm":147,"read_time":46},7971474920318,"https://ap-avatar.wpscdn.com/avatar/74000ee537e6b0ca360?x-image-process=image/resize,m_fixed,w_180,h_180&k=1788430171632181674","Heliyon 9 (2023) e21062  \nContents lists available at ScienceDirect  \nHeliyon  \njournal [homepage:](homepage: www.cell.com/heliyon)[ www.cell.com/heliyon](homepage: www.cell.com/heliyon)  \n| EFHD1 expression is correlated with tumor-infiltrating neutrophils and predicts prognosis in gastric cancer\u003Cbr>Bin Zhao a, 1, Shanshan Wang b, 1, Li Xuea, Qingqing Wang c, Yushan Liu a, Qiang Xu a, Qiu Xued, *\u003Cbr>a Department of Pathology, Nantong Tumor Hospital, Nantong Fifth People’s Hospital, Affiliated Tumor Hospital of Nantong University, Jiangsu, 226361, China\u003Cbr>b Department of General Surgery, The Affiliated Suqian Hospital of Xuzhou Medical University and Nanjing Drum Tower Hospital Group Suqian Hospital, Suqian, 223800, China\u003Cbr>c Department of General Surgery, Affiliated Hospital of Nantong University & Medical School of Nantong University, Jiangsu, 226001, China d Department of General Surgery, Nantong Tumor Hospital, Nantong Fifth People’s Hospital, Affiliated Tumor Hospital of Nantong University, Jiangsu, 226361, China |  |  |\n| --- | --- | --- |\n| A R T I C L E I N F O |  | A B S T R A C T |\n| Keywords:\u003Cbr>EFHD1\u003Cbr>Tumor-infiltrating neutrophils Gastric cancer\u003Cbr>Tumor microenvironment Multiplex immunohistochemistry |  | Background: Gastric cancer (GC) ranks third in terms of mortality worldwide. The tumor microenvironment is critical for the progression of gastric cancer. This study investigated the association between EF-hand domain containing 1 (EFHD1) expression and its clinical significance in the tumor microenvironment (TME) of gastric cancer.\u003Cbr>Methods: We used bioinformatic analyses to assess the relevance of EFHD1 mRNA in the TME of gastric carcinoma tissues and its relationship with clinical features. Therefore, we performed multiplex immunohistochemistry analyses to determine the potential role of the EFHD1 protein in the TME of gastric cancer.\u003Cbr>Results: EFHD1 expression increased dramatically in gastric cancer tissues compared to levels in non-cancerous tissue samples (t = 6.246, P \u003C 0.001). The EFHD1 protein presentation was associated with invasion depth (χ2 = 19. 120, P \u003C 0.001) and TNM stages (χ2 = 14.468, P = 0.002). Notably, EFHD1 protein expression was significantly related to CD66b + neutrophil infiltration of the intratumoral (r = 0.420, P \u003C 0.001) and stromal (r = 0.367, P \u003C 0.001) TME in gastric cancer. Additionally, Cox regression analysis revealed that EFHD1 was an independent prognostic predictor (hazard ratio [HR] = 2.262, P \u003C 0.001) in patients with gastric cancer. Conclusions: Our study revealed the pattern of EFHD1 overexpression in the TME of patients with gastric cancer and demonstrated its utility as a biomarker for unfavorable clinical outcomes, thereby providing a potential immunotherapy target. |\n\n1. Introduction  \nGastric cancer is the most common malignancy of the digestive tract and ranks fifth in incidence and third in cancer-related mortality rates globally [1,2]. Despite advancements in gastric cancer treatment, the survival rate of patients remains low due to  \n* Corresponding author.  \nE-mail address: [xueqiu@ntu.edu.cn](xueqiu@ntu.edu.cn) (Q. Xue).  \n1 These authors contributed equally to this work and share first authorship.  \n[https://doi.org/10.1016/j.heliyon.2023.e21062](https://doi.org/10.1016/j.heliyon.2023.e21062)  \nReceived 16 July 2023; Received in revised form 9 October 2023; Accepted 13 October 2023 Available online 14 October 2023  \n2405-8440/© 2023 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license ([http://creativecommons.org/licenses/by-nc-nd/4.0/](http://creativecommons.org/licenses/by-nc-nd/4.0/)).  \nadvanced-stage diagnosis and therapeutic resistance [3,4]. Therefore, the development of effective predictive biomarkers and treatment targets is essential to prolong the survival of patients with GC [5].  \nThe significance of TME in the initiation and progression of cancer has garnered increasing attention in recen","cbCaihWjtWDUjfUg","https://ap.wps.com/l/cbCaihWjtWDUjfUg","pdf",5397483,"English","# Abstract\n# Introduction","[{\"question\":\"What is the study aimed at determining in gastric cancer?\",\"answer\":\"The study investigates whether EFHD1 expression is associated with the tumor microenvironment and its clinical significance in gastric cancer.\"},{\"question\":\"How do the authors assess EFHD1 in the tumor microenvironment?\",\"answer\":\"They use bioinformatic analyses for EFHD1 mRNA in gastric carcinoma tissues and multiplex immunohistochemistry to evaluate EFHD1 protein within TME compartments.\"},{\"question\":\"What clinical relationships does EFHD1 expression show?\",\"answer\":\"EFHD1 protein presentation correlates with invasion depth and TNM stage and is significantly related to intratumoral and stromal CD66b+ neutrophil infiltration.\"},{\"question\":\"Does EFHD1 have prognostic value for patients with gastric cancer?\",\"answer\":\"Yes. Cox regression analysis indicates EFHD1 is an independent prognostic predictor with a hazard ratio of 2.262 (P \\u003c 0.001).\"}]","EFHD1 expression is correlated with tumor-infiltrating neutrophils and predicts prognosis in gastric cancer | PDF",1790229582]