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Resveratrol suppressed proliferation in a dose- and time-dependent manner, with cytotoxicity observed at 100 μM after 24 hours, reducing viability and inducing apoptosis.",{"@graph":14,"@context":73},[15,34,56],{"@type":16,"itemListElement":17},"BreadcrumbList",[18,23,27,31],{"item":19,"name":20,"@type":21,"position":22},"https://docshare.wps.com","Home","ListItem",1,{"item":24,"name":25,"@type":21,"position":26},"https://docshare.wps.com/document/","Document",2,{"item":28,"name":29,"@type":21,"position":30},"https://docshare.wps.com/document/healthcare/","Healthcare",3,{"item":32,"name":10,"@type":21,"position":33},"https://docshare.wps.com/document/efficacy-of-resveratrol-against-breast-cancer-and-hepatocellular-carcinoma-cell-lines/383407/",4,{"url":32,"name":10,"@type":35,"image":36,"author":41,"headline":10,"publisher":44,"fileFormat":47,"inLanguage":8,"description":12,"dateModified":48,"datePublished":49,"encodingFormat":47,"isAccessibleForFree":50,"interactionStatistic":51},"DigitalDocument",{"url":37,"@type":38,"width":39,"height":40},"https://docshare.wps.com/thumbnails/efficacy-of-resveratrol-against-breast-cancer-and-hepatocellular-carcinoma-cell-lines/383407.png","ImageObject",300,407,{"name":42,"@type":43},"Finn","Person",{"url":19,"name":45,"@type":46},"DocShare","Organization","application/pdf","2026-10-26","2026-09-24",true,{"@type":52,"interactionType":53,"userInteractionCount":55},"InteractionCounter",{"@type":54},"ViewAction",9,{"@type":57,"mainEntity":58},"FAQPage",[59,65,69],{"name":60,"@type":61,"acceptedAnswer":62},"What was the objective of the study?","Question",{"text":63,"@type":64},"To evaluate the anti-cancer effect of resveratrol on MCF-7 and HepG2 cells.","Answer",{"name":66,"@type":61,"acceptedAnswer":67},"How were cell death, proliferation, and apoptosis markers measured?",{"text":68,"@type":64},"Cell death and proliferation were measured using MTT and Trypan blue exclusion assays, and apoptosis markers were assessed by qPCR.",{"name":70,"@type":61,"acceptedAnswer":71},"What were the main findings regarding resveratrol’s effects?",{"text":72,"@type":64},"Resveratrol inhibited proliferation of MCF-7 and HepG2 in a dose- and time-dependent manner, showed cytotoxicity even at 100 μM after 24 hours, reduced viability, and induced apoptosis with elevated apoptosis markers.","https://schema.org",{"og:url":32,"og:type":75,"og:title":10,"og:site_name":45,"og:description":12},"article",{"robots":77,"canonical":32},"index,follow",{"doc_id":79,"site_id":7},383407,1790790026,{"code":4,"msg":82,"data":83},"success",[84,88,92,96,101,106,110,115,119,122,126],{"id":22,"doc_module":4,"doc_module_name":25,"category_name":85,"show_sort_weight":86,"slug":87},"Story & Novel",90,"story-novel",{"id":26,"doc_module":4,"doc_module_name":25,"category_name":89,"show_sort_weight":90,"slug":91},"Literature",80,"literature",{"id":33,"doc_module":4,"doc_module_name":25,"category_name":93,"show_sort_weight":94,"slug":95},"Exam",70,"exam",{"id":97,"doc_module":4,"doc_module_name":25,"category_name":98,"show_sort_weight":99,"slug":100},5,"Comic",60,"comic",{"id":102,"doc_module":4,"doc_module_name":25,"category_name":103,"show_sort_weight":104,"slug":105},6,"Technology",50,"technology",{"id":107,"doc_module":4,"doc_module_name":25,"category_name":29,"show_sort_weight":108,"slug":109},7,40,"healthcare",{"id":111,"doc_module":4,"doc_module_name":25,"category_name":112,"show_sort_weight":113,"slug":114},8,"Research & Report",30,"research-report",{"id":55,"doc_module":4,"doc_module_name":25,"category_name":116,"show_sort_weight":117,"slug":118},"Religion & Spirituality",20,"religion-spirituality",{"id":117,"doc_module":4,"doc_module_name":25,"category_name":120,"show_sort_weight":117,"slug":121},"World Cup","world-cup",{"id":123,"doc_module":4,"doc_module_name":25,"category_name":124,"show_sort_weight":123,"slug":125},10,"Lifestyle","lifestyle",{"id":127,"doc_module":4,"doc_module_name":25,"category_name":128,"show_sort_weight":97,"slug":129},19,"General","general",{"code":4,"msg":82,"data":131},{"doc_id":79,"user_id":132,"nickname":42,"user_avatar":133,"doc_module":4,"category_id":107,"category_name":29,"doc_title":10,"doc_description":12,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":55,"is_deleted":4,"is_public":22,"is_downloadable":22,"audit_status":22,"page_count":107,"language":139,"language_code":8,"site_id":7,"html_lang":8,"table_of_contents":140,"faqs":141,"seo_title":142,"seo_description":12,"update_tm":143,"read_time":144},34359740700684,"https://ap-avatar.wpscdn.com/avatar/1f400023980c374ae676?_k=1777273430885731487","Original Article  \nEfficacy of resveratrol against breast cancer and hepatocellular carcinoma cell lines  \nNoufA. ALkharashi, PhD.  \nABSTRACT  \nالأهداف: تقييم التأثير المضاد للسرطان للريسفيراترول على خلايا مؤسسةميتشيغان للسرطان -7 (MCF-7) و خط خلايا الورم الأرومي الكبدي  \n. )HepG2(  \nالمنهجية: أجريت الدراسة في قسم النبات والأحياء الدقيقة، جامعة الأميرسطام بن عبد العزيز، الخرج، المملكة العربية السعودية، خلال الفترة منأغسطس 2022م إلى أكتوبر 2022م. اضيفت تركيزات مختلفة منريسفيراترول إلى خطوط الخلايا MCF-7 و HepG2. أجرينا قياسموت الخلايا وانتشارها باستخدام مقايسات MTT و Trypan blue exclusion. قيمنا علامات موت الخلايا المبرمج باستخدام مقايسة PCR  \n. )qPCR( الكمي  \nالنتائج: تم إثبات أن ريسفيراترول يثبط تكاثر خلايا MCF-7 و HepG2 حسب الجرعة والوقت. لوحظ التأثير السام للخلايا للريسفيراترول حتى عند 100 ميكرومتر بعد 24 ساعة. بالمقارنة مع الخلايا غير المعالجة، قلل العلاجبالريسفيراترول من صلاحية خلايا MCF-7 إلى ما يقرب من 57.5%بتركيز مثبط أقصى نصف (50IC) من 51.18 ميكرومتر وخلايا HepG2 إلى 56.2% مع 50IC من 57.4 ميكرومتر. علاوة على ذلك، في خطوطالخلايا المختبرة، كان ريسفيراترول قادرًا على إحداث موت الخلايا المبرمجبوساطة علامات موت الخلايا المبرمج المرتفعة.  \nالخلاصة: يبدو أن الريسفيراترول هو عامل مرشح ممتاز في العلاج المضادللسرطان في مختلف أنواع السرطانات البشرية.  \nObjectives: To evaluate the anti-cancer effect of resveratrol on Michigan cancer foundation-7 )MCF-7( and hepatoblastoma cell line )HepG2( cells.  \nMethods: The study was carried out at the Department of Botany and Microbiology, Prince Sattam bin Abdulaziz University, Al-kharj, Saudi Arabia, from August 2022 to October 2022. Different concentrations of resveratrol were added to the MCF-7 and HepG2 cell lines. Cell death and proliferation were measured with MTT and Trypan blue exclusion assays. Apoptosis markers were assessed by using a quantitative PCR assay )qPCR( .  \nResults: The resveratrol was shown to suppress the proliferation of MCF-7 and HepG2 cells at dose-and time-dependent. The cytotoxic effect of resveratrol was observed even at 100 μM after 24 hours. In  \ncomparison to untreated cells, resveratrol treatment reduced the viability of MCF-7 cells to roughly 57.5% with a half maximal inhibitory concentration )IC50( of 51.18 μM and HepG2 cells to 56.2% with an IC50 of 57.4 μM. Furthermore, in the tested cell lines, resveratrol was able to induce apoptosis mediated by elevated apoptosis markers.  \nConclusion: Resveratrol appears to be an excellent candidate agent in anticancer therapy in various human cancers.  \nKeywords: MTT, resveratrol, proliferation, MCF-7, HepG2 cell line  \nSaudi MedJ 2023; Vol. 44 (3): 246-252 doi: 10.15537/smj.2023.44.3.20220768  \nFrom the Department of Home Eonomy, College of Education, Prince Sattam bin Abdulaziz University, Al-kharj, Kingdom of Saudi Arabia.  \nReceived 21st October 2022. Accepted 25th January 2023.  \nAddress correspondence andreprint request to: Dr. NoufA. ALkharashi, Department of Home Eonomy, College of Education, Prince Sattam bin Abdulaziz University, Al-kharj, Kingdom of Saudi Arabia. [E-mail: n.alkarashi@psau.edu.sa](E-mail: n.alkarashi@psau.edu.sa)  \nORCID ID: [https://orcid.org/0000-0002-7782-381X](https://orcid.org/0000-0002-7782-381X)  \nCaonfcermocrtoanlittinyugelsobtoallybetInh2e0m20o, stbrcoeasmcaonncecrauwsaes responsible for almost 685,000 deaths in women globally.1 Currently, the most widely used traditional treatment methods are surgical intervention, radiotherapy, chemotherapy, and immunotherapy, which represent conventional treatment strategies, or a combination of these options to cure cancer, shrink cancer, or stop the progression of cancer, but the availability of effective therapy for cancer is still elusive.2,3 Curative or primary surgery is usually carried out when cancer is found in only one part of the body. Possible complications during surgery may be caused by the surgery itself, drugs used, and the overall health  \n246 [https://smj.org.sa](https://smj.org.sa) Saudi Med J 2023; Vol. 44 )3( OPEN ACCESS  ","cbCaih5RT2YHpKlu","https://ap.wps.com/l/cbCaih5RT2YHpKlu","pdf",970904,"English","# Abstract\n## Objectives\n## Methods\n## Results\n## Conclusion","[{\"question\":\"What was the objective of the study?\",\"answer\":\"To evaluate the anti-cancer effect of resveratrol on MCF-7 and HepG2 cells.\"},{\"question\":\"How were cell death, proliferation, and apoptosis markers measured?\",\"answer\":\"Cell death and proliferation were measured using MTT and Trypan blue exclusion assays, and apoptosis markers were assessed by qPCR.\"},{\"question\":\"What were the main findings regarding resveratrol’s effects?\",\"answer\":\"Resveratrol inhibited proliferation of MCF-7 and HepG2 in a dose- and time-dependent manner, showed cytotoxicity even at 100 μM after 24 hours, reduced viability, and induced apoptosis with elevated apoptosis markers.\"}]","Efficacy of resveratrol against breast cancer and hepatocellular carcinoma cell lines | PDF",1790256671,18]