[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-289996-105":59,"doc-detail-289996-en":131},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":124,"head_meta":126,"extra_data":128,"updated_unix":130},105,"en","effects-of-angiostatin-on-in-vitro-embryonic-rat-development","Effects of Angiostatin on in vitro Embryonic Rat Development","","Angiogenesis progression depends on a balance between positive and negative regulators. This research evaluates the in vitro effects of angiostatin on total embryonic development in rat embryos. Embryos at day 9.5 were cultured in whole rat serum for controls and in whole rat serum supplemented with 0.5, 2.5, or 5 µg/ml angiostatin for experimental groups. After 48 hours, embryos were analyzed morphologically. Growth and development during organogenesis decreased with angiostatin, indicating developmental retardation via antiangiogenic action.",{"@graph":69,"@context":123},[70,84,106],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/effects-of-angiostatin-on-in-vitro-embryonic-rat-development/289996/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/effects-of-angiostatin-on-in-vitro-embryonic-rat-development/289996.png","ImageObject",300,407,{"name":92,"@type":93},"Oliver","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-29","2026-09-17",true,{"@type":102,"interactionType":103,"userInteractionCount":105},"InteractionCounter",{"@type":104},"ViewAction",11,{"@type":107,"mainEntity":108},"FAQPage",[109,115,119],{"name":110,"@type":111,"acceptedAnswer":112},"How was angiostatin tested in this study?","Question",{"text":113,"@type":114},"Rat embryos were explanted on day 9.5 and cultured in whole rat serum, with controls receiving no additive and experimental groups receiving 0.5, 2.5, or 5 µg/ml angiostatin. After 48 hours, embryos were harvested and morphologically analyzed.","Answer",{"name":116,"@type":111,"acceptedAnswer":117},"What was the overall effect of angiostatin on embryonic development?",{"text":118,"@type":114},"Embryonic growth and development during organogenesis decreased in the presence of angiostatin compared with embryos cultured in whole rat serum.",{"name":120,"@type":111,"acceptedAnswer":121},"Which parameters showed reduced values with angiostatin?",{"text":122,"@type":114},"Morphological scores, yolk sac diameters, crown-rump lengths, and protein contents were lower in angiostatin-treated groups. Somite number median values also diminished in the experimental groups.","https://schema.org",{"og:url":83,"og:type":125,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":127,"canonical":83},"index,follow",{"doc_id":129,"site_id":62},289996,1789639503,{"code":4,"msg":5,"data":132},{"doc_id":129,"user_id":133,"nickname":92,"user_avatar":134,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":135,"file_id":136,"file_url":137,"file_type":138,"file_size":139,"view_count":105,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":24,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":130,"read_time":144,"preview_page_count":4},8796095461610,"https://ap-avatar.wpscdn.com/davatar_276721f389ce27ea32af1340a28f341c","Kafkas Univ Vet Fak Derg  \n17 (5): 843-847, 2011  \nDOI:10.9775/kvfd.2011.4637  \nRESEARCH ARTICLE  \nEffects ofAngiostatin on in vitro Embryonic Rat Development  \nTolga ERTEKIN * 􀀇 Harun ULGER * Mehtap NISARI * Omur KARACA ** Erdogan UNUR * Ugur SAHIN *** Ferhan ELMALI ****  \n* Erciyes University, School of Medicine, Department of Anatomy, TR-38039 Kayseri -TURKEY  \n** Balıkesir University, School of Medicine, Department of Anatomy, TR-10145 Balıkesir-TURKEY  \n*** Erciyes University, Faculty of Science, Department of Chemistry, TR-38039 Kayseri -TURKEY  \n**** Erciyes University, School of Medicine, Department of Biostatistic, TR-38039 Kayseri -TURKEY  \n Makale Kodu (Article Code): KVFD-2011-4637   \nSummary  \nThe progression of angiogenesis is controlled by a delicate balance between the positive and negative regulators. Angiostatin has potent antiangiogenic effect on endothelial cells, influencing their proliferation, differentiation and other functions. In this study, in vitro effectsofangiostatin on total embryonic growth were investigated in rat embryos. The rat embryos were explanted on day 9.5 and cultured in whole rat serum (WRS) (for control) and adding 0.5, 2.5 and 5 µg/ml angiostatin (for experimentals) in WRS. After 48 hours culture period, the embryos from each group were harvested and analysed morphologically. The results showed that the embryonic growth and development during organogenesis decreased in the presence ofangiostatin when compared to embryos grown in WRS. Mean morphological scores for the embryos grown in WRS and in the presence of 0.5, 2.5 and 5 µg/ml angiostatin were 59.8±2.02, 34.8±8.52, 22.4±6.41 and 17.8±6.19, yolk sac diameters were 6.0±0.2, 4.8±0.55, 4.5±057 and 4.2±0.39 mm, crown-rump lengths were 5.4±0.23, 4.4±0.42, 4.1±0.87 and 3.7±0.51 mm and mean protein contents of embryos were 140.76±12.21, 112.66±8.67, 89.85±10.89 and 53.77±8.15 µg/ml respectively. Median value of the somite numbers was 24 (24-25) in control group and it was diminished 14 (12- 14), 12 (11-17) and 13 (8-17) respectively in experimental groups. As a result the angiostatin could cause developmental retardation of embryo because of its antiangiogenic effect.  \nKeywords: Angiostatin, Culture, Embryo, in vitro, Rat  \nAnjiostatinin in vitro Embriyonik Rat Gelişimi Üzerine Etkileri Özet  \nAnjiogenezis süreci anjiogenez inhibitör ve aktivatörleri arasındaki denge ile kontrol edilmektedir. Anjiostatin endotel hücrelerin çoğalmasını, farklılaşmasını ve diğer fonksiyonlarını etkileyerek endotel hücreleri üzerinde antianjiogenik etki gösterir. Bu çalışmada, anjiostatin’in sıçan embriyolarında in vitro total embriyonik gelişim süreci üzerine etkileri araştırıldı . 9.5 günlük embriyolar normal sıçan serumunda (kontrol grubu) ve normal sıçan serumuna 0.5, 2.5 ve 5 µg/ml anjiostatin eklenerek (deney grupları) kültüre edildi.  \n48 saatlik kültür periyodu sonunda tüm gruplardaki embriyolar morfolojik olarak değerlendirildi. Deney grupları kontrol grubu ile karşılaştırıldığında organagenezis süresince embriyonik gelişim ve büyümede bir gerilemenin varlığı gözlemlendi. Sıçan serumu vesıçan serumu+0.5, 2.5 ve 5 µg/ml anjiostatin’de kültür edilen embriyolarda ortalama morfolojik skor sırasıyla 59.8±2.02, 34.8±8.52, 22.4±6.41 ve 17.8±6.19, yolk sac çapı 6.0±0.2, 4.8±0.55, 4.5±057 ve 4.2±0.39 mm, baş-kıç uzunluğu 5.4±0.23, 4.4±0.42, 4.1±0.87 ve 3.7±0.51 mm ve ortalama protein içeriği 140.76±12.21, 112.66±8.67, 89.85±10.89 ve 53.77±8.15 µg/ml olarak belirlendi. Ortalama somit sayısı kontrol grubunda 24 (24-25) iken deney gruplarında azalmaktaydı ve sırasıyla 14 (12-14), 12 (11-17) ve 13 (8-17) olarak hesaplandı . Sonuçlar, anjiostatin’in antianjiojenik etkisine bağlı olarak embriyonik gelişimde bir gerilemenin olabileceğini ortaya koymaktadır.  \nAnahtarsözcükler: Anjiostatin, Kültür, Embriyo, in vitro, Sıçan  \nINTRODUCTION  \nDuring embryogenesis, the formation of blood vessels proceeds by both vasculogenesis and angiogenesis. Both  \nprocesses appea","cbCair2z6aTrjrUX","https://ap.wps.com/l/cbCair2z6aTrjrUX","pdf",701088,"English","# Summary\n# Introduction","[{\"question\":\"How was angiostatin tested in this study?\",\"answer\":\"Rat embryos were explanted on day 9.5 and cultured in whole rat serum, with controls receiving no additive and experimental groups receiving 0.5, 2.5, or 5 µg/ml angiostatin. After 48 hours, embryos were harvested and morphologically analyzed.\"},{\"question\":\"What was the overall effect of angiostatin on embryonic development?\",\"answer\":\"Embryonic growth and development during organogenesis decreased in the presence of angiostatin compared with embryos cultured in whole rat serum.\"},{\"question\":\"Which parameters showed reduced values with angiostatin?\",\"answer\":\"Morphological scores, yolk sac diameters, crown-rump lengths, and protein contents were lower in angiostatin-treated groups. Somite number median values also diminished in the experimental groups.\"}]","Effects of Angiostatin on in vitro Embryonic Rat Development | PDF",13]