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Cells are labeled using antibodies recognizing CD33 and CD66b surface markers and magnetic particles, enabling separation without columns by an EasySep™ magnet. Isolated cells are available for downstream flow cytometry, culture, or DNA/RNA extraction. The kit includes defined cocktail, nanoparticles, and 10X RBC lysis buffer with specified storage and stability.",{"@graph":69,"@context":114},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/easysep-human-whole-blood-myeloid-positive-selection-kit-catalog-18683-version-123/500322/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/easysep-human-whole-blood-myeloid-positive-selection-kit-catalog-18683-version-123/500322.png","ImageObject",300,407,{"name":92,"@type":93},"วิน","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-09","2026-10-03",true,{"@type":102,"interactionType":103,"userInteractionCount":14},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108],{"name":109,"@type":110,"acceptedAnswer":111},"What storage conditions and shelf-life guidance are provided for kit components?","Question",{"text":112,"@type":113},"The selection cocktail and magnetic nanoparticles are stored at 2–8°C and must not be frozen, while the RBC lysis buffer concentrate is stored at 15–25°C and must not be frozen. Stability is described as stable until the expiry date (EXP) on each label, with additional guidance for 1X dilution of RBC lysis buffer stored at 2–8°C.","Answer","https://schema.org",{"og:url":83,"og:type":116,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":118,"canonical":83},"index,follow",{"doc_id":120,"site_id":62},500322,1791506049,{"code":4,"msg":5,"data":123},{"doc_id":120,"user_id":124,"nickname":92,"user_avatar":125,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":126,"file_id":127,"file_url":128,"file_type":129,"file_size":130,"view_count":14,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":19,"language":131,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":132,"faqs":133,"seo_title":134,"seo_description":67,"update_tm":135,"read_time":52},2336475104736,"https://ap-avatar.wpscdn.com/avatar/22000c4c5e0e5b17e70?x-image-process=image/resize,m_fixed,w_180,h_180&k=1786591360781797222","This document [is available at www.stemcell.com/PIS](is available at www.stemcell.com/PIS)  \nEasySep™ Human Whole Blood Myeloid Positive  \nPositive Selection Selection Kit  \nCatalog \\#18683 For processing 60 mL whole blood  \nDocument \\#28986 | Version 1_2_3  \nDescription  \nIsolate highly purified whole blood myeloid cells from fresh whole blood by immunomagnetic positive selection.  \n􀁸 Fast and easy-to-use  \n􀁸 Up to 99% purity  \n􀁸 No columns required  \nThis kit targets whole blood myeloid cells for positive selection with an antibody recognizing the CD33 and CD66b surface markers. Desired cells are labeled with antibodies and magnetic particles, and separated without columns using an EasySep™ magnet. Unwanted cells are simply poured off, while desired cells remain in the tube. Isolated cells are immediately available for downstream applications such as flow cytometry, culture, or DNA/RNA extraction.  \nComponent Descriptions  \n\n| COMPONENT NAME | COMPONENT \\# | QUANTITY | STORAGE | SHELF LIFE | FORMAT |\n| --- | --- | --- | --- | --- | --- |\n| EasySep™ Human Whole Blood Myeloid Positive Selection Cocktail | 18683C | 3 x 1 mL | Store at 2-8°C.\u003Cbr>Do not freeze. | Stable until expiry date (EXP) on label. | A combination of monoclonal antibodies in PBS. |\n| EasySep™ Whole Blood Magnetic Nanoparticles | 18180 | 3 x 1 mL | Store at 2-8°C.\u003Cbr>Do not freeze. | Stable until expiry date (EXP) on label. | A suspension of magnetic particles in water. |\n| EasySep™ Red Blood Cell Lysis Buffer, 10X Concentrate | 20110 | 1 x 10 mL | Store at 15-25°C. Do not freeze. | Stable until expiry date (EXP) on label. | A 10X concentrated red blood cell lysis reagent. |\n\nPBS-phosphate-buffered saline  \nComponents may be shipped at room temperature (15-25°C) but should be stored as indicated above.  \nAdditional Reagent Stability Information  \n\n| REAGENT NAME | STORAGE | SHELF LIFE |\n| --- | --- | --- |\n| EasySep™ Red Blood Cell Lysis Buffer (1X dilution) | Store at 2-8°C. Do not freeze. | Stable for up to 3 months. Do not exceed the expiry date (EXP) of the original component. |\n\nSample Preparation  \nFor available fresh and frozen samples, [see www.stemcell.com/primarycells](see www.stemcell.com/primarycells).  \nPERIPHERAL BLOOD  \nCollect whole blood in a blood collection tube containing anticoagulant.  \nRecommended Medium  \nEasySep™ Buffer (Catalog \\#20144), RoboSep™ Buffer (Catalog \\#20104) , or PBS containing 2% fetal bovine serum (FBS) and 1 mM EDTA. Medium should be free of Ca++ and Mg++ .  \n\n| \u003Cbr>EasySep™ Human Whole Blood Myeloid Positive Selection Kit\u003Cbr>Positive Selection |  |  |  |\n| --- | --- | --- | --- |\n| Directions for Use – Manual EasySep™ Protocols\u003Cbr>See page 1 for Sample Preparation and Recommended Medium. Refer to Table 1 for detailed instructions regarding the EasySep™ procedure.\u003Cbr>Table 1. EasySep™ Human Whole Blood Myeloid Positive Selection Kit Protocol |  |  |  |\n|  |  | EASY SEP™ MAGNET |  |\n| STEP | INSTRUCTIONS | “The Big Easy”\u003Cbr>(Catalog \\#18001) | |\n| 1 | Prepare sample within the volume range. | Up to 4.5 mL |  |\n|  | Add sample to required tube. | 14 mL (17 x 100 mm) polystyrene round-bottom tube (e.g. Corning Catalog \\#352057) |  |\n| 2 | Add 1X EasySep™ RBC Lysis Buffer to sample. | Equal volume to sample |  |\n| 3 | Add Selection Cocktail to sample. | 25 μL/mL of diluted sample |  |\n|  | Mix and incubate. | RT for 15 minutes |  |\n| 4 | Mix Magnetic Particles.\u003Cbr>NOTE: Particles should appear evenly dispersed. | Pipette up and down more than 5 times |  |\n| 5 | Add Magnetic Particles to sample. | 25 μL/mL of diluted sample |  |\n|  | Mix and incubate. | RT for 10 minutes |  |\n| 6 | Add recommended medium to top up the sample to the indicated volume. Mix by gently pipetting up and down 2 3 times. | 􀁸 Top up to 5 mL for samples \u003C 2.5 mL\u003Cbr>􀁸 Top up to 10 mL for samples ≥ 2.5 mL |  |\n|  | Place the tube (without lid) into the magnet and incubate. | RT for 10 minutes |  |\n| 7 | Pick up the magnet, and in one continuous motion ","cbCaipfFN1zlaIPe","https://ap.wps.com/l/cbCaipfFN1zlaIPe","pdf",294156,"English","# Component Descriptions\n## Additional Reagent Stability Information\n# Sample Preparation\n## Peripheral Blood\n## Recommended Medium\n# Directions for Use – Manual EasySep™ Protocols\n## Table 1 Protocol Steps\n# Directions for Use – Fully Automated RoboSep™ Protocol\n## Table 2 Protocol Steps","[{\"question\":\"What storage conditions and shelf-life guidance are provided for kit components?\",\"answer\":\"The selection cocktail and magnetic nanoparticles are stored at 2–8°C and must not be frozen, while the RBC lysis buffer concentrate is stored at 15–25°C and must not be frozen. Stability is described as stable until the expiry date (EXP) on each label, with additional guidance for 1X dilution of RBC lysis buffer stored at 2–8°C.\"}]","EasySep™ Human Whole Blood Myeloid Positive Selection Kit - Catalog 18683 - Version 1.2.3 | PDF",1791010440]