[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-329702-105":59,"doc-detail-329702-en":131},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":124,"head_meta":126,"extra_data":128,"updated_unix":130},105,"en","early-in-vitro-results-indicate-that-de-o-acetylated-sialic-acids-increase-selectin-binding-in-cancers","Early in vitro results indicate that de-O-acetylated sialic acids increase Selectin binding in cancers","","Early in vitro results identify de-O-acetylated sialic acids as functional modifiers of the Sialic Acid–Selectin axis used during cancer metastasis. The study focuses on how deviations from canonical Sia structure regulate binding to Selectin receptors (E, L, and P) on hemopoietic cells. Using CRISPR-Cas9 gene editing to modulate O-acetylation states and sialyl glycan recognition probes to generate O-acetylated or de-O-acetylated Sia, experiments show increased cell-surface expression and higher Selectin binding, proliferation, and migration in lung and colon cancer models. Mechanistic evidence supports glycan functional-group specificity for therapeutic development, including glycan-based therapeutic strategies such as enzyme–antibody drug conjugates.",{"@graph":69,"@context":123},[70,84,106],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/early-in-vitro-results-indicate-that-de-o-acetylated-sialic-acids-increase-selectin-binding-in-cancers/329702/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/early-in-vitro-results-indicate-that-de-o-acetylated-sialic-acids-increase-selectin-binding-in-cancers/329702.png","ImageObject",300,407,{"name":92,"@type":93},"Genevieve","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-11-12","2026-09-21",true,{"@type":102,"interactionType":103,"userInteractionCount":105},"InteractionCounter",{"@type":104},"ViewAction",11,{"@type":107,"mainEntity":108},"FAQPage",[109,115,119],{"name":110,"@type":111,"acceptedAnswer":112},"What question does the study address about sialic acid and Selectins?","Question",{"text":113,"@type":114},"The work examines how non-canonical deviations in the Sia structure regulate binding to Selectin receptors (E, L, and P) and thereby contribute to metastatic processes.","Answer",{"name":116,"@type":111,"acceptedAnswer":117},"How were O-acetylated vs de-O-acetylated sialic acid forms generated for experiments?",{"text":118,"@type":114},"CRISPR-Cas9 gene editing was used to modulate 7,9-O and 9-Oacetylation states of Sia, and sialyl glycan recognition probes were used to produce and detect the O-acetylated or de-O-acetylated forms.",{"name":120,"@type":111,"acceptedAnswer":121},"What in vitro effects were observed when de-O-acetylated Sia increased on cell surfaces?",{"text":122,"@type":114},"In vitro results showed increased Selectin binding, enhanced cell proliferation, and improved migration capabilities in lung and colon cancer.","https://schema.org",{"og:url":83,"og:type":125,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":127,"canonical":83},"index,follow",{"doc_id":129,"site_id":62},329702,1789996481,{"code":4,"msg":5,"data":132},{"doc_id":129,"user_id":133,"nickname":92,"user_avatar":134,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":135,"file_id":136,"file_url":137,"file_type":138,"file_size":139,"view_count":105,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":56,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":145,"preview_page_count":4},1374391974585,"https://ap-avatar.wpscdn.com/davatar_276721f389ce27ea32af1340a28f341c","TYPE Original Research PUBLISHED 09 December 2024 DOI 10.3389/fonc.2024.1443303  \nOPEN ACCESS  \nEDITED BY  \nLubor Borsig,  \nUniversity of Zurich, Switzerland  \nREVIEWED BY  \nRoger Chammas,  \nUniversity of São Paulo, Brazil Giselle Hevia Hernandez, University of Bern, Switzerland  \n*CORRESPONDENCE  \nRachel Willand-Charnley  \n [rachel.willand@sdstate.edu](rachel.willand@sdstate.edu)  \n†These authors have contributed equally to this work  \nRECEIVED 03 June 2024  \nACCEPTED 11 November 2024  \nPUBLISHED 09 December 2024  \nCITATION  \nDas K, Schulte M, Gerhart M, Bayoumi H, Paulson D, Fink DM, Parrish C and Willand-Charnley R (2024) Early in vitro results indicate that de-O-acetylated sialic acids increase Selectin binding in cancers. Front. Oncol. 14:1443303 .  \ndoi: 10.3389/fonc.2024.1443303  \nCOPYRIGHT  \n© 2024 Das, Schulte, Gerhart, Bayoumi, Paulson, Fink, Parrish and Willand-Charnley. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) . The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.  \nEarly in vitro results indicate that de-O-acetylated sialic acids increase Selectin binding in cancers  \nKakali Das 1†, Megan Schulte 1†, Megan Gerhart 1†, Hala Bayoumi 1, Delayna Paulson 1, Darci M. Fink 1, Colin Parrish 2  \nand Rachel Willand-Charnley1*  \n1 Department of Chemistry, Biochemistry and Physics, South Dakota State University, Brookings, SD, United States, 2 Department of Microbiology and Immunology,Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, Ithaca, NY, United States  \nCancers utilize a simple glycan, Sialic Acid, to engage in metastatic processes via the Sialic acid (Sia) -Selectin pathway. Selectins recognize and bind to sialylated substrates, resulting in adhesion, migration, and extravasation, however, how deviations from the canonical form of Sia regulate binding to Selectin receptors (E, L, and P) on hemopoietic cells resulting in these metastatic processes, remained a gap in knowledge. De-O-acetylated Sias has been recently shown to be an integral substrate to the binding of sialic acid binding proteins. The two proteins responsible for regulating the acetyl functional group on Sia’s C6 tail, are Sialic acid acetylesterase (SIAE) and Sialic acid O acetyltransferase (CASD1) . To elucidate the contribution of functional group alterations on Sia, 9-O and 7,9-Oacetylation of Sia was modulated via the use of CRISRP-Cas9 gene editing and with Sialyl Glycan Recognition Probes, to produce either O-acetylated-Sia or deO-acetylated-Sia, respectively. In vitro experiments revealed that increased cell surface expression of de-O-acetylated-Sia resulted in an increase in Selectin binding, enhanced cell proliferation, and increased migration capabilities both in lung and colon cancer. These results delineate for the first time the mechanistic contribution of de-O-acetylated-Sia to Selectin binding, reinforcing the importance of elucidating functional group alterations on Sia and their contribution. Without accurate identification of which functionalized form of Sia is being utilized to bind to sialic acid binding proteins, we cannot accurately produce glycan therapeutics with the correct specificity and reactivity, thus this work contributes an integral component in the development of promising therapeutic avenues, for example in the realm of enzyme antibody drug conjugates.  \nKEYWORDS  \nde-O-acetylated sialic acid, cancer, selectins, sia-selectin pathway, Sialyl Lewis X, PSGL-1, metastasis, migration  \nFrontiers in Oncology 01 [frontiersin.org](frontiersin.org)  \nIntroduction  \nCancer uses simple sugar residues to engage in metastatic processes (1) via the Sel","cbCaijkZAWGukwyn","https://ap.wps.com/l/cbCaijkZAWGukwyn","pdf",16221525,"English","# Introduction\n## Cancer metastasis and the Selectin–sialic acid pathway\n## Non-canonical sialic acid as a Selectin ligand\n## Rationale for targeting Sia functional-group acetylation states","[{\"question\":\"What question does the study address about sialic acid and Selectins?\",\"answer\":\"The work examines how non-canonical deviations in the Sia structure regulate binding to Selectin receptors (E, L, and P) and thereby contribute to metastatic processes.\"},{\"question\":\"How were O-acetylated vs de-O-acetylated sialic acid forms generated for experiments?\",\"answer\":\"CRISPR-Cas9 gene editing was used to modulate 7,9-O and 9-Oacetylation states of Sia, and sialyl glycan recognition probes were used to produce and detect the O-acetylated or de-O-acetylated forms.\"},{\"question\":\"What in vitro effects were observed when de-O-acetylated Sia increased on cell surfaces?\",\"answer\":\"In vitro results showed increased Selectin binding, enhanced cell proliferation, and improved migration capabilities in lung and colon cancer.\"}]","Early in vitro results indicate that de-O-acetylated sialic acids increase Selectin binding in cancers | PDF",1789985246,48]