[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-381043-105":59,"doc-detail-381043-en":131},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":124,"head_meta":126,"extra_data":128,"updated_unix":130},105,"en","dual-probe-difference-specimen-imaging-for-prostate-cancer-margin-assessment","Dual probe difference specimen imaging for prostate cancer margin assessment","","Positive surgical margins after radical prostatectomy for high-risk localized prostate cancer can require reoperation or adjuvant therapy, increasing morbidity and mortality, while treatment side effects further highlight the need for effective initial cure. Current intraoperative tumor margin assessment relies mainly on frozen section analysis, which is slow, labor-intensive, and disruptive in the operating room. Dual probe difference specimen imaging (DDSI) uses paired antibody probes with spectrally distinct fluorophores and ratiometric difference imaging to rapidly assess PSMA status as a surrogate for margin status.",{"@graph":69,"@context":123},[70,84,106],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/dual-probe-difference-specimen-imaging-for-prostate-cancer-margin-assessment/381043/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/dual-probe-difference-specimen-imaging-for-prostate-cancer-margin-assessment/381043.png","ImageObject",300,407,{"name":92,"@type":93},"Hazel","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-27","2026-09-24",true,{"@type":102,"interactionType":103,"userInteractionCount":105},"InteractionCounter",{"@type":104},"ViewAction",12,{"@type":107,"mainEntity":108},"FAQPage",[109,115,119],{"name":110,"@type":111,"acceptedAnswer":112},"Why are positive margins a critical problem in prostate cancer surgery?","Question",{"text":113,"@type":114},"Positive margins indicate incomplete tumor removal after radical prostatectomy for high-risk localized prostate cancer, often requiring reoperation or adjuvant therapy, which increases morbidity and mortality.","Answer",{"name":116,"@type":111,"acceptedAnswer":117},"What limitation of frozen section analysis motivates DDSI?",{"text":118,"@type":114},"Frozen section analysis is lengthy, labor-intensive, and requires a pathologist in the operating room, along with workflow disruption and variability in sensitivity and specificity.",{"name":120,"@type":111,"acceptedAnswer":121},"How does DDSI determine prostate cancer margin status?",{"text":122,"@type":114},"DDSI uses two antibody-based probes—one targeted to PSMA and one untargeted—then calculates ratiometric differences between targeted and untargeted probe uptake to highlight PSMA-positive tumor versus normal tissue patterns.","https://schema.org",{"og:url":83,"og:type":125,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":127,"canonical":83},"index,follow",{"doc_id":129,"site_id":62},381043,1790332360,{"code":4,"msg":5,"data":132},{"doc_id":129,"user_id":133,"nickname":92,"user_avatar":134,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":135,"file_id":136,"file_url":137,"file_type":138,"file_size":139,"view_count":105,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":140,"language":141,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":142,"faqs":143,"seo_title":144,"seo_description":67,"update_tm":145,"read_time":146},137441390410,"https://ap-avatar.wpscdn.com/avatar/2000252f4ab5702993?_k=1776741390130283984","Dual probe difference specimen imaging for prostate cancer margin assessment  \nMarcus J. Kwon ,a Broderick J. House,a Connor W. Barth ,a Allison Solanki,a Jocelyn A. Jones ,a Scott C. Davis,c and Summer L. Gibbsa,b,*  \naOregon Health & Science University, Biomedical Engineering Department, Portland,  \nOregon, United States  \nbKnight Cancer Institute, Oregon Health & Science University, Portland, Oregon, United States cThayer School of Engineering at Dartmouth College, Hanover, New Hampshire, United States  \nAbstract  \nSignificance: Positive margin status due to incomplete removal of tumor tissue during radical prostatectomy for high-risk localized prostate cancer requires reoperation or adjuvant therapy, which increases morbidity and mortality. Adverse effects of prostate cancer treatments commonly include erectile dysfunction, urinary incontinence, and bowel dysfunction, making successful initial curative prostatectomy imperative.  \nAim: Current intraoperative tumor margin assessment is largely limited to frozen section analysis, which is a lengthy, labor-intensive process that is obtrusive to the clinical workflow within the operating room (OR) . Therefore, a rapid method for prostate cancer margin assessment in the OR could improve outcomes for patients.  \nApproach: Dual probe difference specimen imaging (DDSI), which uses paired antibody-based probes that are labeled with spectrally distinct fluorophores, was shown herein for prostate cancer margin assessment. The paired antibody-based probes consisted of a targeted probe to prostate-specific membrane antigen (PSMA) and an untargeted probe, which were used as a cocktail to stain resected murine tissue specimens including prostate tumor, adipose, muscle, and normal prostate. Ratiometric images (i.e., DDSI) of the difference between targeted and untargeted probe uptake were calculated and evaluated for accuracy using receiver operator characteristic curve analysis with area under the curve values used to evaluate the utility of the DDSI method to detect PSMA positive prostate cancer.  \nResults: Targeted and untargeted probe uptake was similar between the high and low PSMA expressing tumor due to nonspecific probe uptake after topical administration. The ratiometric DDSI approach showed substantial contrast difference between the PSMA positive tumors and their respective normal tissues (prostate, adipose, muscle) . Furthermore, DDSI showed substantial contrast difference between the high PSMA expressing tumors and the minimally PSMA expressing tumors due to the ratiometric correction for the nonspecific uptake patterns in resected tissues.  \nConclusions: Previous work has shown that ratiometic imaging has strong predictive value for breast cancer margin status using topical administration. Translation of the ratiometric DDSI methodology herein from breast to prostate cancers demonstrates it as a robust, ratiometric technique that provides a molecularly specific imaging modality for intraoperative margin detection. Using the validated DDSI protocol on resected prostate cancers permitted rapid and accurate assessment of PSMA status as a surrogate for prostate cancer margin status. Future studies will further evaluate the utility of this technology to quantitatively characterize prostate margin status using PSMA as a biomarker.  \n© The Authors. Published by SPIE under a Creative Commons Attribution 4.0 International License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI. [DOI: 10.1117/1.JBO.28.8.082806]  \n*Address all correspondence to Summer L. Gibbs, [gibbss@ohsu.edu](gibbss@ohsu.edu)  \nJournal of Biomedical Optics 082806-1 August 2023 • Vol. 28(8)  \nKeywords: dual probe difference specimen imaging; prostate cancer; prostatectomy; tumor margin assessment; fluorescence guided surgery.  \nPaper 220159GRRR received Jul. 15, 2022; accepted for publication Mar. 31, 2023; published online Apr. 18","cbCaigOKG4lrPzx2","https://ap.wps.com/l/cbCaigOKG4lrPzx2","pdf",3334052,14,"English","# Introduction\n## Significance and clinical need\n## Current intraoperative margin assessment limitations\n## Approach overview (DDSI)\n# Conclusions","[{\"question\":\"Why are positive margins a critical problem in prostate cancer surgery?\",\"answer\":\"Positive margins indicate incomplete tumor removal after radical prostatectomy for high-risk localized prostate cancer, often requiring reoperation or adjuvant therapy, which increases morbidity and mortality.\"},{\"question\":\"What limitation of frozen section analysis motivates DDSI?\",\"answer\":\"Frozen section analysis is lengthy, labor-intensive, and requires a pathologist in the operating room, along with workflow disruption and variability in sensitivity and specificity.\"},{\"question\":\"How does DDSI determine prostate cancer margin status?\",\"answer\":\"DDSI uses two antibody-based probes—one targeted to PSMA and one untargeted—then calculates ratiometric differences between targeted and untargeted probe uptake to highlight PSMA-positive tumor versus normal tissue patterns.\"}]","Dual probe difference specimen imaging for prostate cancer margin assessment | PDF",1790243283,35]