[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-402466-105":59,"doc-detail-402466-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","dna-isolation-with-qiagen-genomic-tips-protocol-for-neuroblastoma-nbl-and-wilms-tumor-wt","DNA Isolation with Qiagen Genomic-Tips - Protocol for Neuroblastoma (NBL) and Wilms Tumor (WT)","","Protocol detailing genomic DNA isolation from snap-frozen tumor or normal tissues for Neuroblastoma (NBL; before 2013) and Wilms Tumor (WT). Describes the rationale and workflow using QIAGEN Blood & Cell Culture DNA Kits and QIAGEN Genomic-tips with Genomic DNA Buffer Set: optimized lysis, anion-exchange resin binding under controlled low-salt/pH, removal of RNA/proteins/impurities by washing, and elution with high-salt buffer followed by isopropanol precipitation for concentration and desalting. Includes specimen handling, required equipment and reagents, warnings, storage conditions, quality control labeling, and stepwise procedure guidance aligned with COG and NCI TARGET usage.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/dna-isolation-with-qiagen-genomic-tips-protocol-for-neuroblastoma-nbl-and-wilms-tumor-wt/402466/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/dna-isolation-with-qiagen-genomic-tips-protocol-for-neuroblastoma-nbl-and-wilms-tumor-wt/402466.png","ImageObject",300,407,{"name":92,"@type":93},"iuabcfuw","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-11-05","2026-09-27",true,{"@type":102,"interactionType":103,"userInteractionCount":34},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What is the principle behind the Qiagen Genomic-tips DNA isolation method described here?","Question",{"text":112,"@type":113},"The method lyses cells and/or nuclei, binds genomic DNA to anion exchange resin under low salt and pH, removes RNA/proteins/dyes and low-molecular-weight impurities by washing, then elutes genomic DNA and concentrates/desalts it using isopropanol precipitation.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"What specimen handling conditions are required before DNA isolation?",{"text":117,"@type":113},"Standard precautions must be followed for neuroblastoma tissue, and samples must be stored at -80°C prior to DNA isolation.",{"name":119,"@type":110,"acceptedAnswer":120},"How is specimen identification and labeling handled for quality control?",{"text":121,"@type":113},"A specific color is assigned through each phase to match the tube and column used throughout the procedure. NBL and Wilms tumor specimens also receive additional identifiers such as NBL/ACC numbers, patient initials, COG number, and BPC number.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},402466,1790619523,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":34,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":145,"preview_page_count":4},3985747874027,"https://ap-avatar.wpscdn.com/davatar_9964176cb1d06d4a9deccf72a44ae3dc","DNA Isolation with Qiagen Genomic-Tips  \nThis protocol applies to: Neuroblastoma (NBL; prior to 2013), and Wilms  \nTumor (WT)  \nThe protocol herein describes the procedures used by Nationwide Children’s Hospital to process disease tissues for RNA and/or DNA subsequently used for characterization in the NCI’s TARGET initiative. All nucleic acid samples used in TARGET projects were quality tested for consistency using picogreen quantification and SSTR genotyping methods, regardless of where the nucleic acid was originally extracted.  \nI. PRINCIPLE  \nQIAGEN Blood & Cell Culture DNA Kits and QIAGEN Genomic-tips with the Genomic DNA Buffer Set , provide an easy, safe and reliable method for the isolation of pure high molecular weight genomic DNA, direct from whole blood, lymphocytes and tissues. The procedure is based on optimized buffer system for lysis of cells and/or nuclei, followed by binding of genomic DNA to QIAGEN Anion Exchange Resin under appropriate low salt and pH conditions. RNA, proteins, dyes and lowmolecular-weight impurities are removed by a medium-salt wash. Genomic DNA iseluted in a high-salt buffer and concentrated and desalted by isopropanol precipitation.  \nII. SPECIMEN  \nA. Type  \nTumor or normal tissue – snap frozen  \nB. Handling Conditions  \nStandard precautions must be followed when handling all neuroblastoma tissue. Samples must be stored at-80􀁱 C prior to DNA isolation.  \nC. Sample Preparation  \nTissue is obtained from the BPC in a 2 mL safe-lock tube. The weight of tissue requested is 50-60 mg.  \nD. Indications for Study  \nDNA isolated from tissue is to be used for Children’s Oncology Group (COG) related research; this protocol is most commonly used for LOH analysis.  \nIII. REQUIRED EQUIPMENT, SUPPLIES AND REAGENTS  \nA. Equipment  \nCentrifuge, Capsule  \nCentrifuge, Table Top with 15 mL and 50 mL tube holders  \nPipet (electric)  \nPipetters-Adjustable, 1-10 􀁐L, 5-20 􀁐L, 20-200 􀁐L, 100-1000 􀁐L  \nVortex Mixer  \nWater Bath at 37°C  \nWater Bath at 50°C  \nTissueLyser (120V, 50/60 Hz) (Qiagen, Catalog\\# 85210)  \nTissueLyser Adapter sets (2 X 24)  \nTEC label printer  \nB. Supplies  \nQIArack  \nIce Bucket  \nFloating Tube Rack  \nForceps, disposable (Fisher, Catalog\\# F-1205-2)  \nAerosol Barrier Pipet Tips  \nMarkers  \n15 and 50 mL Conical Bottom Polypropylene Centrifuge Tubes  \nPPE (Gloves, Lab Coat, Safety Glasses)  \nLabels  \n2 mL Safe-Lock Tubes (Eppendorf, Catalog\\# 2236335-2)  \n1.5 mL Microcentrifuge Tubes  \n2 mL Microcentrifuge Tubes  \nMicrocentrifuge Tube Rack  \nRacks  \nSterile Plastic Pipets for Large Volumes (2, 5, 10 and 25 mL)  \nDry Ice  \nStainless Steel Beads, 5mm (Qiagen, Catalog\\# 69989)  \n1.5 mL Micrewtubes, round bottom (Milian, Catalog\\# T338-5S)  \n0.5 mL Micrewtubes, self-standing (Milian, Catalog\\# T338-2S) Blue inserts (Milian, TIL-28)  \nRed inserts (Milian, TIL-14)  \nPipet tips (20 μL, 200 μL, 1000 μL)  \nReach pipet tips (1000 μL)  \nTEC printer labels  \nC. Supplies for tissue smashing (alternate method)  \nSafety shield  \nDull rubber hammer  \nSuper-Polyfoil, 4X4, 100/box (Fisher, Catalog\\# 22029118)  \nDewars (2)  \nLiquid Nitrogen  \nHearing Protection  \n14” Tweezers  \nD. Reagents  \nQIAGEN Blood & Cell Culture DNA Kit Midi (Qiagen, Catalog\\# 13343)  \n70% Ethanol -Stored at-20􀁱 C (Sigma-Aldrich, Catalog\\# E7023)  \nIsopropanol (Sigma, Catalog\\# I0398)  \nWater Molecular Biology Reagent (Sigma, Catalog\\# W4502)  \nTris-EDTA Buffer 100X Concentrate (Sigma, Catalog\\# T9285)  \nQIAGEN Proteinase K (Qiagen, Catalog\\# 19133)  \nRNase A (100 mg/mL) (Qiagen, Catalog\\# 19101)  \nIV. WARNINGS / PRECAUTIONS  \nA. Use Standard Precautions when handling all body fluids, tissues and cell cultures. Refer to the Specimen Handling and Collection procedure, GEN-1, for guidelines specific for the Molecular Genetics Laboratory and samples.  \nV. REAGENT PREPARATION (INCLUDING STORAGE CONDITIONS)  \nA. The QIAGEN Blood & Cell Culture DNA Kits and QIAGEN Genomic-tips andreagents are used within one year of opening.  \nB. The water a","cbCaiq5xPMRDfomy","https://ap.wps.com/l/cbCaiq5xPMRDfomy","pdf",268865,11,"English","# I. PRINCIPLE\n# II. SPECIMEN\n## A. Type\n## B. Handling Conditions\n## C. Sample Preparation\n## D. Indications for Study\n# III. REQUIRED EQUIPMENT, SUPPLIES AND REAGENTS\n## A. Equipment\n## B. Supplies\n## C. Supplies for tissue smashing (alternate method)\n## D. Reagents\n# IV. WARNINGS / PRECAUTIONS\n## A. Use Standard Precautions\n# V. REAGENT PREPARATION (INCLUDING STORAGE CONDITIONS)\n## A. Kit and reagent opening timelines\n## B. Storage and use conditions for water and buffers\n## C. Isopropanol and ethanol preparation/storage\n# VI. QUALITY CONTROL\n## A. Specimen identification and labeling\n## B. Sample opening control\n## C. Retention of non-DNA supernatant/pellet\n## D. Compliance and QA testing\n# VII. PROCEDURE – STEPWISE\n## A. NBL DNA Isolation Worksheet","[{\"question\":\"What is the principle behind the Qiagen Genomic-tips DNA isolation method described here?\",\"answer\":\"The method lyses cells and/or nuclei, binds genomic DNA to anion exchange resin under low salt and pH, removes RNA/proteins/dyes and low-molecular-weight impurities by washing, then elutes genomic DNA and concentrates/desalts it using isopropanol precipitation.\"},{\"question\":\"What specimen handling conditions are required before DNA isolation?\",\"answer\":\"Standard precautions must be followed for neuroblastoma tissue, and samples must be stored at -80°C prior to DNA isolation.\"},{\"question\":\"How is specimen identification and labeling handled for quality control?\",\"answer\":\"A specific color is assigned through each phase to match the tube and column used throughout the procedure. NBL and Wilms tumor specimens also receive additional identifiers such as NBL/ACC numbers, patient initials, COG number, and BPC number.\"}]","DNA Isolation with Qiagen Genomic-Tips - Protocol for Neuroblastoma (NBL) and Wilms Tumor (WT) | PDF",1790530745,28]