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It provides a table of contents covering restriction enzyme digestion background, DNA sequencing, electrophoresis setup and running, agarose gel staining and data analysis, and student pre-lab activities such as strawberry DNA extraction and pipetting practice. 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DNA extraction from strawberry ..… Teacher guide: DNA extraction from strawberry................…........….... 14  \nStudent guide: DNA extraction from strawberry................…........….... 16  \nStudent Pre-Lab Activity: Pipetting practice and practice gel loading ..………… 17  \nExperiment  \nAnalyzing DNA using electrophoresis …………………………………………................... 18  \nStaining an agarose gel with methylene blue ….………………....………….…………… 19  \nAnalyzing data from an agarose gel .....……...................................................... 20  \nCleaning up …………………………......……............................................................... 22  \nAppendices  \nAppendix table of contents ..……...…....…..........................…….......................... 23  \nDirections for pipettors ………….....................................................………........... 24  \nConducting electrophoresis: Setting up the gel box .........……......................... 26  \nConducting electrophoresis: Pouring gels ………….........…….............................. 27  \nConducting electrophoresis: Running the gel ………........……............................ 29  \nDirections for power supplies ……...…..............................……........................... 30  \nPlasmid and DNA ladder information ……………..…..............................…………... 31  \nConceptual review material ..……...…..............................……............................ 33  \nTechnical review material ..……...…..............................……......................………. 36  \nScenarios and case studies ..……...…..............................…….............................. 42  \nScience education standards ..……...…..............................……........................... 59  \nSources for materials & equipment ...…..............................……........................ 62  \nSolutions ……………...…….....……...…..............................……................................ 64  \nReferences …………...…….....……...…..............................……................................ 64  \nDirections for return shipping ..……...…..............................……......................... 65  \nGraph paper …………………..……...…..............................……................................. 66  \nWHAT’S NEW?  \nOur twentieth year!  \nBelieve it or not, the 2013-2014 academic year will be the 20th year for the Biotech-in-a-Box program. We started with a single DNA kit (and eight borrowers, all retired now!) in 1994. Last year, the DNA kits were borrowed by 133 teachers and used with over 9,000 students! Overall, the program now offers 6 different kits and has provided over 230,000 lab experiences to Virginia students in the past 19 years.  \nUpdated manual!  \nThere are only a few changes, but an updated look!  \nBack to TAE buffer!  \nWe’re switching back to TAE buffer. The gels will be prepared with ","cbCaiqrg7NrnQA3h","https://ap.wps.com/l/cbCaiqrg7NrnQA3h","pdf",1152555,66,"English","# Introduction\n## What’s new this year?\n## DNA Biotechnology Kit contents\n## Calendar of activities\n## Important notes (Please read!)\n## Digesting DNA with restriction enzymes: background information\n## DNA sequencing\n## Conducting electrophoresis: Background information\n## Student Pre-Lab Activity: What is DNA? DNA extraction from strawberry\n## Student Pre-Lab Activity: Pipetting practice and practice gel loading\n# Experiment\n## Analyzing DNA using electrophoresis\n## Staining an agarose gel with methylene blue\n## Analyzing data from an agarose gel\n## Cleaning up\n# Appendices\n## Appendix table of contents\n## Directions for pipettors\n## Conducting electrophoresis: Setting up the gel box\n## Conducting electrophoresis: Pouring gels\n## Conducting electrophoresis: Running the gel\n## Directions for power supplies\n## Plasmid and DNA ladder information\n## Conceptual review material\n## Technical review material\n## Scenarios and case studies\n## Science education standards\n## Sources for materials & equipment\n## Solutions\n## References\n## Directions for return shipping\n## Graph paper","[{\"question\":\"What is the loan period for the equipment in the DNA Biotechnology Kit?\",\"answer\":\"The loan period is two weeks, and longer loans may be arranged for multiple teachers at the same school.\"},{\"question\":\"What updates were made for the 2013–2014 year?\",\"answer\":\"The manual includes a switch back to TAE buffer with specified buffer concentrations for gel preparation and running, removes the heavy books/DVDs, makes the DNA ladder optional, and addresses light box availability.\"},{\"question\":\"Which lab activities does the manual cover for analyzing DNA?\",\"answer\":\"It covers restriction enzyme digestion background, DNA sequencing, electrophoresis setup and running, staining agarose gels with methylene blue, analyzing agarose gel data, and cleaning up after experiments.\"}]","DNA Biotechnology Kit - Information Manual - Student and Lab Activities | PDF",1791021422,166]