[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-333667-105":59,"doc-detail-333667-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","distinctive-field-effects-of-smoking-and-lung-cancer-case-control-status-on-bronchial-basal-cell-growth-and-signaling","Distinctive field effects of smoking and lung cancer case-control status on bronchial basal cell growth and signaling","","Rational basal cells (BCs) are bronchial progenitor/stem cells that regenerate injured airways in smokers, where malignant transformation may occur. Using this model for early lung carcinogenesis, the study characterizes cytologically normal BC outgrowths from never-smokers and ever-smokers without cancer controls, and from the epithelial “field” of ever-smokers with anatomically remote cancers as cases, spanning lung adenocarcinoma (LUAD) and squamous cell carcinoma (LUSC).",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/distinctive-field-effects-of-smoking-and-lung-cancer-case-control-status-on-bronchial-basal-cell-growth-and-signaling/333667/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/distinctive-field-effects-of-smoking-and-lung-cancer-case-control-status-on-bronchial-basal-cell-growth-and-signaling/333667.png","ImageObject",300,407,{"name":92,"@type":93},"Oliver","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-26","2026-09-21",true,{"@type":102,"interactionType":103,"userInteractionCount":39},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What cellular model did the study use to examine early lung carcinogenesis?","Question",{"text":112,"@type":113},"The study used bronchial basal cells (BCs) as a model for early stages of lung carcinogenesis and characterized cytologically normal BC outgrowths from different donor groups.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How were BCs collected and analyzed in the experiments?",{"text":117,"@type":113},"Primary BCs were cultured from endobronchial brushings taken remote from visible lesions or tumors, and donor subgroups were tested using qRT-PCR, RNA sequencing, flow cytometry, immunofluorescence, and immunoblot.",{"name":119,"@type":110,"acceptedAnswer":120},"How did smoking and lung cancer case-control status affect BC proliferation and phenotype?",{"text":121,"@type":113},"Smoking reduced overall BC proliferation, corresponding to a lower EpCAMpos/ITGA6pos/CD24pos stem cell fraction, while LUSC showed increased dysmorphic BCs and LUAD donors displayed increased proliferation and S-phase fractions.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},333667,1790379856,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":39,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":145,"preview_page_count":4},8796095461610,"https://ap-avatar.wpscdn.com/davatar_276721f389ce27ea32af1340a28f341c","Zefi et al. Respiratory Research (2024) 25:317 [https://doi.org/10.1186/s12931-024-02924-w](https://doi.org/10.1186/s12931-024-02924-w)  \nRespiratory Research  \nRESEARCH Open Access  \nDistinctive field effects of smoking and lung  cancer case-control status on bronchial basal cell growth and signaling  \nOlsida Zefi 1,2, Spencer Waldman 1,2,3, Ava Marsh3, Miao Kevin Shi3, Yosef Sonbol ian 1,2, Batbayar Khulan3,  \nTaha Siddiqui3, Aditi Desai3, Dhruv Patel3, Aham Okorozo3, Samer Khader3, Jay Dobkin3, Ali Sadoughi3, Chirag Shah3, Simon Spivack3† and Yakov Peter 1,2,3,4*†  \nAbstract  \nRational Basal cells (BCs) are bronchial progenitor/stem cells that can regenerate injured airway that, in smokers, may undergo malignant transformation. As a model for early stages of lung carcinogenesis, we set out to characterize cytologically normal BC outgrowths from never-smokers and ever-smokers without cancers (controls), as well as from the normal epithelial “field” of ever-smokers with anatomically remote cancers, including lung adenocarcinoma (LUAD) and squamous cell carcinoma (LUSC) (cases) .  \nMethods Primary BCs were cultured and expanded from endobronchial brushings taken remote from the site of clinical or visible lesions/tumors. Donor subgroups were tested for growth, morphology, and underlying molecular features by qRT-PCR, RNAseq, flow cytometry, immunofluorescence, and immunoblot.  \nResults (a) the BC population includes epithelial cell adhesion molecule (EpCAM) positive and negative cell subsets; (b) smoking reduced overall BC proliferation corresponding with a 2. 6-fold reduction in the EpCAMpos/ ITGA6 pos/CD24pos stem cell fraction; (c) LUSC donor cells demonstrated up to 2. 8-fold increase in dysmorphic BCs; and (d) cells procured from LUAD patients displayed increased proliferation and S-phase cell cycle fractions. These differences corresponded with: (i) disparate NOTCH1/NOTCH2 transcript expression and altered expression of potential downstream (ii) E-cadherin (CDH1), tumor protein-63 (TP63), secretoglobin family 1a member 1 (SCGB1A1), and Hairy/ enhancer-of-split related with YRPW motif 1 (HEY1); and (iii) reduced EPCAM and increased NK2 homeobox-1 (NKX2-1) mRNA expression in LUAD donor BCs.  \nConclusions These and other findings demonstrate impacts of donor age, smoking, and lung cancer case-control status on BC phenotypic and molecular traits and may suggest Notch signaling pathway deregulation during early human lung cancer pathogenesis.  \nKeywords Basal cells, Bronchial brushing, Cancer field, Carcinogenesis, Non-small cell lung cancer, Adenocarcinoma, Squamous cell carcinoma, Notch signaling, EpCAM, NKX2-1, ITGA6, CD24, KRT14  \n†Simon Spivack and Yakov Peter are Co-senior authors.  \n*Correspondence: Yakov Peter [yakov.peter@touro.edu](yakov.peter@touro.edu)  \nFull list of author information is available at the end of the article  \n© The Author(s) 2024. Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit [http://](http://)[ ](http://)[creativecommons.org/l](creativecommons.org/l)icenses/by-nc-nd/4.0/.  \nIntroduction  ","cbCaid0OQC361eMy","https://ap.wps.com/l/cbCaid0OQC361eMy","pdf",9329297,13,"English","# Abstract\n# Methods\n# Results\n# Conclusions\n# Introduction","[{\"question\":\"What cellular model did the study use to examine early lung carcinogenesis?\",\"answer\":\"The study used bronchial basal cells (BCs) as a model for early stages of lung carcinogenesis and characterized cytologically normal BC outgrowths from different donor groups.\"},{\"question\":\"How were BCs collected and analyzed in the experiments?\",\"answer\":\"Primary BCs were cultured from endobronchial brushings taken remote from visible lesions or tumors, and donor subgroups were tested using qRT-PCR, RNA sequencing, flow cytometry, immunofluorescence, and immunoblot.\"},{\"question\":\"How did smoking and lung cancer case-control status affect BC proliferation and phenotype?\",\"answer\":\"Smoking reduced overall BC proliferation, corresponding to a lower EpCAMpos/ITGA6pos/CD24pos stem cell fraction, while LUSC showed increased dysmorphic BCs and LUAD donors displayed increased proliferation and S-phase fractions.\"}]","Distinctive field effects of smoking and lung cancer case-control status on bronchial basal cell growth and signaling | PDF",1790003547,33]