[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-455514-105":59,"doc-detail-455514-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","diminished-osteoprotegerin-production-by-gingival-fibroblasts-from-periodontitis-patients-is-associated-with-their-reduced-ability-to-suppress-osteoclastogenesis","Diminished osteoprotegerin production by gingival fibroblasts from periodontitis patients is associated with their reduced ability to suppress osteoclastogenesis","","Osteoclast-mediated alveolar bone resorption drives periodontitis progression, yet how gingival fibroblasts (GFs) influence osteoclastogenesis remains debated. The study analyzed mediators secreted by GFs from healthy donors versus periodontitis patients and assessed osteoclast formation. Conditioned media from healthy GFs strongly suppressed RANKL-induced osteoclastogenesis via osteoprotegerin (OPG), while periodontitis-derived GFs produced less OPG and showed reduced inhibitory activity. OPG knockdown in periodontitis GFs further weakened the suppression.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/diminished-osteoprotegerin-production-by-gingival-fibroblasts-from-periodontitis-patients-is-associated-with-their-reduced-ability-to-suppress-osteoclastogenesis/455514/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/diminished-osteoprotegerin-production-by-gingival-fibroblasts-from-periodontitis-patients-is-associated-with-their-reduced-ability-to-suppress-osteoclastogenesis/455514.png","ImageObject",300,407,{"name":92,"@type":93},"Eliana","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-11-04","2026-09-30",true,{"@type":102,"interactionType":103,"userInteractionCount":34},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"How did conditioned media from healthy gingival fibroblasts affect osteoclast formation?","Question",{"text":112,"@type":113},"Conditioned media from healthy donor GFs strongly suppressed RANKL-induced osteoclastogenesis.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"What difference was observed in osteoprotegerin production between periodontitis and healthy individuals?",{"text":117,"@type":113},"GFs from periodontitis patients produced significantly less OPG than GFs from healthy individuals.",{"name":119,"@type":110,"acceptedAnswer":120},"How did silencing OPG in periodontitis fibroblasts influence osteoclastogenesis?",{"text":121,"@type":113},"siRNA-mediated silencing of OPG in periodontitis patients’ GFs further decreased their inhibitory effect on osteoclast formation.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},455514,1790977400,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":34,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":145,"preview_page_count":4},4398048949847,"https://ap-avatar.wpscdn.com/avatar/400002536579ef2da7f?_k=1778318612642679267","[www. nature.com/scientificreports](www. nature.com/scientificreports)  \nOPEN  \nDiminished osteoprotegerin production by gingival fibroblasts from periodontitis patients is associated with their reduced ability to suppress osteoclastogenesis  \nElwira Nieboga1,2, Kamila Drzazga1,2, Alicja Plonczynska1,2, Dominika M. Drapala1,2, Aureliusz Schuster1,2, Mariia Melnykova1,2, Julia Skawska2,3, Michal Zajdel4, Malgorzata Kantorowicz5, Sanne Roffel6, Joanna Cichy3, Marta Czesnikiewicz-Guzik5,7, Tomasz Kaczmarzyk4, Susan Gibbs6,8, Jan Potempa1,9 & Aleksander M. Grabiec1􀀍  \nOsteoclast-mediated alveolar bone resorption is a key hallmark of periodontitis. Gingival fibroblasts (GFs) play multifaceted roles in the progression of chronic inflammation; however, their involvement in osteoclastogenesis remains controversial. Here, we analyzed the effects of mediators secreted by GFs from healthy donors and periodontitis patients on osteoclast formation. We observed strong suppression of receptor activator of nuclear factor kappa-B ligand (RANKL)-induced osteoclastogenesis by conditioned media from healthy donor GFs, which was independent of any prior in vitro stimulation or infection. These media contained high levels of osteoprotegerin (OPG), and GFs were identified asthe mainOPG source in gingival tissue using a 3D organotypic reconstructed human gingiva model anda single-cell RNA-seq dataset. Notably, GFs from periodontitis patients produced significantly less OPG compared to GFs from healthy individuals and were less effective at suppressing osteoclastogenesis. Finally, siRNA-mediated silencing of OPG expression in GFs from periodontitis patients further decreased their inhibitory effect on osteoclast formation, confirming the central role of OPG inosteoclastogenesis regulation by GFs. Collectively, these results demonstrate that in healthy gingival tissue, GFs exert bone-protective effects by inhibiting osteoclast formation. However, prolonged exposure to the microenvironment of the inflamed gingival tissue could impair this protective function through sustained reduction of OPG production, which may contribute to alveolar bone resorption in periodontitis.  \nPeriodontitis is a chronic inflammatory disease of the periodontium that affects approximately 11% of the global population in its advanced stages1. It not only causes tooth loss but is also associated with numerous systemic diseases2. While the disease is initiated by microbial imbalance, it is the host’s excessive immune response that  \n1Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland. 2Doctoral School of Exact and Natural Sciences, Jagiellonian University, Kraków, Poland.  \n3Department of Immunology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland. 4Chair of Oral Surgery, Faculty of Medicine, Jagiellonian University Medical College, Kraków, Poland. 5Department of Periodontology, Preventive Dentistry and Oral Medicine, Faculty of Medicine, Jagiellonian University Medical College, Kraków, Poland. 6Department of Oral Cell Biology, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and Vrije Universiteit Amsterdam, Amsterdam, The Netherlands.  \n7Oral Sciences Research Group, School of Medicine, Dentistry & Nursing, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, UK. 8Department of Molecular Cell Biology and Immunology, Amsterdam Infection and Immunity Institute, Amsterdam University Medical Centre Location Vrije Universiteit Amsterdam, Amsterdam, The Netherlands. 9Department of Oral Immunology and Infectious Diseases, University of Louisville School of Dentistry, Louisville, KY, USA. 􀀍 email: [aleksander.grabiec@uj.edu.pl](aleksander.grabiec@uj.edu.pl)  \n[www. nature.com/scientificreports/](www. nature.com/scientificreports/)  \ndrives chronic inflammation3. Immunopathological processes in the periodontium, charac","cbCaim5qCUNdCLn4","https://ap.wps.com/l/cbCaim5qCUNdCLn4","pdf",5858660,13,"English","# Background\n## Periodontitis and bone resorption\n## RANKL/OPG axis and osteoclast regulation\n## Role of gingival fibroblasts\n# Mechanistic Rationale\n## Inflammatory mediators and cellular sources\n## Hypothesis on OPG contribution\n# Experimental Approach (as described)\n## Mediators secreted by GFs and osteoclast formation","[{\"question\":\"How did conditioned media from healthy gingival fibroblasts affect osteoclast formation?\",\"answer\":\"Conditioned media from healthy donor GFs strongly suppressed RANKL-induced osteoclastogenesis.\"},{\"question\":\"What difference was observed in osteoprotegerin production between periodontitis and healthy individuals?\",\"answer\":\"GFs from periodontitis patients produced significantly less OPG than GFs from healthy individuals.\"},{\"question\":\"How did silencing OPG in periodontitis fibroblasts influence osteoclastogenesis?\",\"answer\":\"siRNA-mediated silencing of OPG in periodontitis patients’ GFs further decreased their inhibitory effect on osteoclast formation.\"}]","Diminished osteoprotegerin production by gingival fibroblasts from periodontitis patients is associated with their reduced ability to suppress osteoclastogenesis | PDF",1790743294,33]