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MiRNA levels were measured by real-time PCR, while HR-HPV DNA was quantified with digital-droplet PCR. Among 11 miRNAs, hsa-miR-25-5p was lower in HPV-infected ovarian tissues, and hsa-miR-218-5p was higher in high-grade serous ovarian carcinoma. In stage III/IV disease, hsa-miR-218-5p increased and hsa-miR-191-5p decreased. Unsupervised clustering revealed three patient groups with distinct HPV16/18 positivity patterns and miRNA profiles. 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Wilczy ´nski 4,5, Katarzyna D. Kania 1, Daria Har˛ea 1,6, Andrzej Malinowski 3, Ewelina Perdas 2, Mateusz Nowak 5 and Edyta Paradowska 1, *  \nCitation: Jarych, D.; Mikulski, D.; Wilczy ´nski, M.; Wilczy ´nski, J.R.; Kania, K.D.; Har˛ez˙ a, D.; Malinowski, A.; Perdas, E.; Nowak, M.; Paradowska, E. Differential microRNA Expression Analysis in Patients with HPV-Infected Ovarian Neoplasms. Int. J. Mol. Sci. 2024, 25, 762. [https://doi.org/10.3390/](https://doi.org/10.3390/)[ ](https://doi.org/10.3390/)ijms25020762  \nAcademic Editors: Christine Kottaridi, Dimitris Tsakogiannis and Zaharoula Kyriakopoulou  \nReceived: 1 December 2023  \nRevised: 31 December 2023  \nAccepted: 4 January 2024  \nPublished: 7 January 2024  \nCopyright: © 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license ([https://](https://)[ ](https://)[creativecommons.org/licenses/by/](creativecommons.org/licenses/by/)[ ](creativecommons.org/licenses/by/)[4.0/](4.0/)) .  \n1 Laboratory of Virology, Institute of Medical Biology, Polish Academy of Sciences, 93-232 Lodz, Poland; [djarych@cbm.pan.pl](djarych@cbm.pan.pl) (D.J.); [kkania@cbm.pan.pl](kkania@cbm.pan.pl) (K.D.K.); [dhareza@cbm.pan.pl](dhareza@cbm.pan.pl) (D.H.)  \n2 Department of Biostatistics and Translational Medicine, Medical University of Lodz, 92-215 Lodz, Poland; [damian.mikulski@stud.umed.lodz.pl](damian.mikulski@stud.umed.lodz.pl) (D.M.); [ewelina.perdas@umed.lodz.pl](ewelina.perdas@umed.lodz.pl) (E.P.)  \n3 Department of Surgical, Endoscopic and Oncological Gynecology, Institute of the Polish Mother's Health Center, 93-338 Lodz, Poland; [milosz.wilczynski@iczmp.edu.pl](milosz.wilczynski@iczmp.edu.pl) (M.W.); [amalinowski@kki.pl](amalinowski@kki.pl) (A.M.)  \n4 Department of Surgical and Oncological Gynecology, Medical University of Lodz, 90-419 Lodz, Poland; [jrwil@post.pl](jrwil@post.pl)  \n5 Department of Gynecology and Obstetrics, Tomaszow Health Center, 97-200 Tomaszow Mazowiecki, Poland; [mat.nnowak@gmail.com](mat.nnowak@gmail.com)  \n6 Bio-Med-Chem Doctoral School of University of Lodz and Lodz Institutes of the Polish Academy of Sciences, 90-136 Lodz, Poland  \n* Correspondence: [eparadowska@cbm.pan.pl](eparadowska@cbm.pan.pl); Tel.: +48-42-272-36-29  \nAbstract: This study aimed to identify microRNAs (miRNAs) whose expression levels are altered by high-risk human papillomavirus (HR-HPV) infection in women with epithelial ovarian neoplasms. MiRNA expression was quantified by real-time polymerase chain reaction, while HR-HPV DNA was quantified using digital-droplet PCR. Analysis of 11 miRNAs demonstrated significantly lower hsa-miR-25-5p expression in HPV-infected compared to uninfected ovarian tissues (p = 0.0405), while differences in miRNA expression in corresponding serum were statistically insignificant. The expression of hsa-miR-218-5p in ovarian tumors was significantly higher in high-grade serous ovarian carcinoma (HGSOC) cases than in other neoplasms (p = 0.0166) . In addition, hsa-miR-218-5p was significantly upregulated, whereas hsa-miR-191-5p was significantly downregulated in tissues with stage III/IV FIGO (p = 0.0009 and p = 0.0305, respectively) . Using unsupervised clustering, weidentified three unique patient groups with significantly varied frequencies of HPV16/18-positive samples and varied miRNA expression profiles. In multivariate analysis, high expression of hsa-miR- 16-5p was an independent prognostic factor for poor overall survival (p = 0.0068) . This preliminary analysis showed the changes in miRNA expression in ovarian neoplasms during HPV infection and those collected from HGSOCs or patients with advanced disease. This prospective study can provide new insights into the pathogenesis of","cbCairEunFtiq3ES","https://ap.wps.com/l/cbCairEunFtiq3ES","pdf",1906599,17,"English","# Introduction\n## MicroRNAs and HPV-associated ovarian neoplasms","[{\"question\":\"What association was found between microRNA expression and overall survival?\",\"answer\":\"Higher hsa-miR-16-5p expression was an independent prognostic factor for poor overall survival in multivariate analysis.\"}]","Differential microRNA Expression Analysis in Patients with HPV-Infected Ovarian Neoplasms | PDF",1789975367,43]