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This work develops targeted colonoscopic fluorescence imaging to improve contrast between colonic adenomas and surrounding normal epithelium. It reports Pluronic® micellar nanoparticles loaded with fluorocoxib A (FA) as a fluorescent COX-2 inhibitor, plus a non-targeted fluorophore control, demonstrating strong detection and COX-2–dependent tumor retention in mouse models.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/development-of-pluronic-nanoparticles-of-fluorocoxib-a-for-endoscopic-fluorescence-imaging-of-colonic-adenomas-abstract-methods-and-results/382292/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/development-of-pluronic-nanoparticles-of-fluorocoxib-a-for-endoscopic-fluorescence-imaging-of-colonic-adenomas-abstract-methods-and-results/382292.png","ImageObject",300,407,{"name":92,"@type":93},"Aria","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-11-03","2026-09-24",true,{"@type":102,"interactionType":103,"userInteractionCount":34},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"Why is fluorescence imaging proposed instead of standard white-light colonoscopy?","Question",{"text":112,"@type":113},"White-light colonoscopy has a high false-negative rate, allowing a substantial fraction of preneoplastic lesions to remain undetected, contributing to interval malignancies.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"What is fluorocoxib A (FA) in this study?",{"text":117,"@type":113},"FA is a fluorescent, selective COX-2 inhibitor designed for targeted imaging of inflammation and cancer, enabling visualization of adenomas via COX-2–linked fluorescence.",{"name":119,"@type":110,"acceptedAnswer":120},"How were FA nanoparticles formulated and tested?",{"text":121,"@type":113},"FA (and a fluorocoxib negative control) were formulated into Pluronic micellar nanoparticles using a bulk solvent evaporation method and evaluated in mice bearing colon adenomas using colonoscopic fluorescence imaging.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},382292,1790934749,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":34,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":39,"language":139,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":140,"faqs":141,"seo_title":142,"seo_description":67,"update_tm":143,"read_time":46},2336464648322,"https://ap-avatar.wpscdn.com/avatar/2200025388227c56fec?_k=1778556882303663488","LETTER  \nDevelopment of Pluoronic nanoparticles of fluorocoxib A for endoscopic fluorescence imaging of colonic adenomas  \nMd. Jashim Uddin,a,* Hiroaki Niitsu,b Robert J. Coffey,c and Lawrence J. Marnetta,d,e,*  \naVanderbilt University School of Medicine, Department of Biochemistry, Nashville, Tennessee,  \nUnited States  \nbVanderbilt University Medical Center, Department of Medicine, Nashville, Tennessee,  \nUnited States  \ncVanderbilt University Medical Center, Division of Gastroenterology, Hepatology and Nutrition,  \nDepartment of Medicine, Nashville, Tennessee, United States dVanderbilt University, Department of Chemistry, Nashville, Tennessee, United States eVanderbilt University School of Medicine, Department of Pharmacology, Nashville, Tennessee,  \nUnited States  \nAbstract  \nSignificance: Current white light colonoscopy suffers from many limitations that allow 22% to 32% of preneoplastic lesions to remain undetected. This high number of false negatives contributes to the appearance of interval malignancies, defined as neoplasms diagnosed between screening colonoscopies at a rate of 2% to 6% .  \nAim: The shortcomings of today’s white light-based colorectal cancer screening are addressed by colonoscopic fluorescence imaging of preneoplastic lesions using targeted fluorescent agents to enhance contrast between the lesion and the surrounding normal colonic epithelium. Approach: We describe the development of Pluronic® nanoparticles of fluorocoxib A (FA), a fluorescent cyclooxygenase-2 (COX-2) inhibitor that enables targeted imaging of inflammation and cancer in numerous animal models, for endoscopic florescence imaging of colonicadenomas.  \nResults: We formulated FA, a fluorescent COX-2 inhibitor, or fluorocoxib negative control (FNC), a nontargeted fluorophore and a negative control for FA, in micellar nanoparticles of FDA approved Pluronic tri-block co-polymer using a bulk solvent evaporation method. This afforded FA-loaded micellar nanoparticles (FA-NPs) or FNC-loaded micellar nanoparticles (FNC-NPs) with the hydrodynamic diameters (Dh ) of 45.7 􀀁 2.5 nm and 44.9 􀀁 3.8 nm and the zeta potentials (ζ) of −1.47 􀀁 0.3 mV and −1.64 􀀁 0.5 mV, respectively. We intravenously injected B6;129 mice bearing colonic adenomas induced by azoxymethane and dextran-sodium sulfate with FA-loaded Pluronic nanoparticles (FA-NPs) . The diffusion-mediated local FA release and its binding to COX-2 enzyme allowed for clear detection of adenomas with high signal-to-noise ratios. The COX-2 targeted delivery and tumor retention were validated by negligible tumor fluorescence detected upon colonoscopic imaging of adenoma-bearing mice injected with Pluronic nanoparticles of FNC or of animals predosed with the COX-2 inhibitor, celecoxib, followed by intravenous dosing of FA-NPs.  \nConclusions: These results demonstrate that the formulation of FA in Pluronic nanoparticles overcomes a significant hurdle to its clinical development for early detection of colorectal neoplasms by fluorescence endoscopy.  \n© The Authors. Published by SPIE under a Creative Commons Attribution 4.0 International License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI. [DOI: 10.1117/1.JBO.28.4.040501]  \nKeywords: Fluorocoxib A; Pluronic® nanoparticles; cyclooxygenase-2; colorectal adenomas; fluorescence colonoscopy.  \n*Address all correspondence to Md. Jashim Uddin, [jashim.uddin@vanderbilt.edu](jashim.uddin@vanderbilt.edu); Lawrence J. Marnett: [larry.marnett@vanderbilt.edu](larry.marnett@vanderbilt.edu)  \nJournal of Biomedical Optics 040501-1 April 2023 • Vol. 28(4)  \nUddin et al.: Development of Pluoronic nanoparticles of fluorocoxib A for endoscopic fluorescence imaging. . .  \nPaper 220308LR received Jan. 12, 2023; accepted for publication Mar. 30, 2023; published online Apr. 20, 2023.  \n1 Introduction  \nThe enzyme, cyclooxygenase-2 (COX-2), is overexpressed in a wide variety of malignant ","cbCaihfaWNMpt9uH","https://ap.wps.com/l/cbCaihfaWNMpt9uH","pdf",622192,"English","# Abstract\n## Significance\n## Aim\n## Approach\n## Results\n## Conclusions\n# Introduction\n# Materials and Methods","[{\"question\":\"Why is fluorescence imaging proposed instead of standard white-light colonoscopy?\",\"answer\":\"White-light colonoscopy has a high false-negative rate, allowing a substantial fraction of preneoplastic lesions to remain undetected, contributing to interval malignancies.\"},{\"question\":\"What is fluorocoxib A (FA) in this study?\",\"answer\":\"FA is a fluorescent, selective COX-2 inhibitor designed for targeted imaging of inflammation and cancer, enabling visualization of adenomas via COX-2–linked fluorescence.\"},{\"question\":\"How were FA nanoparticles formulated and tested?\",\"answer\":\"FA (and a fluorocoxib negative control) were formulated into Pluronic micellar nanoparticles using a bulk solvent evaporation method and evaluated in mice bearing colon adenomas using colonoscopic fluorescence imaging.\"}]","Development of Pluronic nanoparticles of fluorocoxib A for endoscopic fluorescence imaging of colonic adenomas - Abstract, Methods, and Results | PDF",1790249888]