[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"doc-seo-383046-105":3,"detail-sidebar-cat-0-en-105":81,"doc-detail-383046-en":131},{"code":4,"msg":5,"data":6},0,"ok",{"site_id":7,"language":8,"slug":9,"title":10,"keywords":11,"description":12,"schema_data":13,"social_meta":74,"head_meta":76,"extra_data":78,"updated_unix":80},105,"en","development-of-novel-tracers-for-sentinel-node-identification-in-cervical-cancer","Development of novel tracers for sentinel node identification in cervical cancer","","Indocyanine green (ICG) with near-infrared fluorescence imaging supports lymphatic mapping, yet binding to serum proteins can reduce retention time in sentinel lymph nodes (SLNs). A new fluorescence tracer was evaluated: phytate combined with liposome (LP)-encapsulated ICG (ICG-phytate-LP). Phytate with LP containing phosphatidic acid promotes Ca2+-mediated chelation and enhances SLN retention. In mice, ICG-phytate-LP showed the strongest lymph node retention profile over 6 h and durable signal thereafter, and pigs receiving ICG-phytate-LP demonstrated good overall safety and no weight loss. The tracer may aid gynecological cancer SLN identification, especially with retroperitoneal approaches.",{"@graph":14,"@context":73},[15,34,56],{"@type":16,"itemListElement":17},"BreadcrumbList",[18,23,27,31],{"item":19,"name":20,"@type":21,"position":22},"https://docshare.wps.com","Home","ListItem",1,{"item":24,"name":25,"@type":21,"position":26},"https://docshare.wps.com/document/","Document",2,{"item":28,"name":29,"@type":21,"position":30},"https://docshare.wps.com/document/healthcare/","Healthcare",3,{"item":32,"name":10,"@type":21,"position":33},"https://docshare.wps.com/document/development-of-novel-tracers-for-sentinel-node-identification-in-cervical-cancer/383046/",4,{"url":32,"name":10,"@type":35,"image":36,"author":41,"headline":10,"publisher":44,"fileFormat":47,"inLanguage":8,"description":12,"dateModified":48,"datePublished":49,"encodingFormat":47,"isAccessibleForFree":50,"interactionStatistic":51},"DigitalDocument",{"url":37,"@type":38,"width":39,"height":40},"https://docshare.wps.com/thumbnails/development-of-novel-tracers-for-sentinel-node-identification-in-cervical-cancer/383046.png","ImageObject",300,407,{"name":42,"@type":43},"Kurz","Person",{"url":19,"name":45,"@type":46},"DocShare","Organization","application/pdf","2026-10-29","2026-09-24",true,{"@type":52,"interactionType":53,"userInteractionCount":55},"InteractionCounter",{"@type":54},"ViewAction",11,{"@type":57,"mainEntity":58},"FAQPage",[59,65,69],{"name":60,"@type":61,"acceptedAnswer":62},"Why is enhancing sentinel lymph node retention important in cervical cancer imaging?","Question",{"text":63,"@type":64},"SLN biopsy helps determine whether lymph nodes are metastatic, guiding the need for adjuvant therapy. Earlier metastasis detection is important, and avoiding unnecessary lymph node dissection can reduce complications such as lymphedema and lymphangitis.","Answer",{"name":66,"@type":61,"acceptedAnswer":67},"How does the new tracer ICG-phytate-LP improve SLN retention?",{"text":68,"@type":64},"Coadministration of phytate with liposomes containing phosphatidic acid promotes Ca2+-mediated chelation in bodily fluids, improving SLN retention. Uniform ICG-loaded liposomes were produced while minimizing fluorescence self-quenching during storage.",{"name":70,"@type":61,"acceptedAnswer":71},"What safety and efficacy results were observed in mice and pigs?",{"text":72,"@type":64},"In mice, ICG-phytate-LP provided the best lymph node retention, with fluorescence intensity increasing over 6 hours before slowly decreasing over the next 24 hours. In pigs, administration of ICG and ICG-phytate-LP caused no death or weight loss, and blood tests showed no obvious differences between groups, indicating good overall safety.","https://schema.org",{"og:url":32,"og:type":75,"og:title":10,"og:site_name":45,"og:description":12},"article",{"robots":77,"canonical":32},"index,follow",{"doc_id":79,"site_id":7},383046,1790794572,{"code":4,"msg":82,"data":83},"success",[84,88,92,96,101,106,110,115,120,123,127],{"id":22,"doc_module":4,"doc_module_name":25,"category_name":85,"show_sort_weight":86,"slug":87},"Story & Novel",90,"story-novel",{"id":26,"doc_module":4,"doc_module_name":25,"category_name":89,"show_sort_weight":90,"slug":91},"Literature",80,"literature",{"id":33,"doc_module":4,"doc_module_name":25,"category_name":93,"show_sort_weight":94,"slug":95},"Exam",70,"exam",{"id":97,"doc_module":4,"doc_module_name":25,"category_name":98,"show_sort_weight":99,"slug":100},5,"Comic",60,"comic",{"id":102,"doc_module":4,"doc_module_name":25,"category_name":103,"show_sort_weight":104,"slug":105},6,"Technology",50,"technology",{"id":107,"doc_module":4,"doc_module_name":25,"category_name":29,"show_sort_weight":108,"slug":109},7,40,"healthcare",{"id":111,"doc_module":4,"doc_module_name":25,"category_name":112,"show_sort_weight":113,"slug":114},8,"Research & Report",30,"research-report",{"id":116,"doc_module":4,"doc_module_name":25,"category_name":117,"show_sort_weight":118,"slug":119},9,"Religion & Spirituality",20,"religion-spirituality",{"id":118,"doc_module":4,"doc_module_name":25,"category_name":121,"show_sort_weight":118,"slug":122},"World Cup","world-cup",{"id":124,"doc_module":4,"doc_module_name":25,"category_name":125,"show_sort_weight":124,"slug":126},10,"Lifestyle","lifestyle",{"id":128,"doc_module":4,"doc_module_name":25,"category_name":129,"show_sort_weight":97,"slug":130},19,"General","general",{"code":4,"msg":82,"data":132},{"doc_id":79,"user_id":133,"nickname":42,"user_avatar":134,"doc_module":4,"category_id":107,"category_name":29,"doc_title":10,"doc_description":12,"doc_content":135,"file_id":136,"file_url":137,"file_type":138,"file_size":139,"view_count":55,"is_deleted":4,"is_public":22,"is_downloadable":22,"audit_status":22,"page_count":116,"language":140,"language_code":8,"site_id":7,"html_lang":8,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":12,"update_tm":144,"read_time":145},2336478945635,"https://ap-avatar.wpscdn.com/davatar_6f874abed73319feea01a86fa6f0fab8","DOI: 10. 1111/cas.15927  \nOR I G I NAL ART I C L E  \nDevelopment of novel tracers for sentinel node identification in cervical cancer  \nKeisuke Kodama1  | Chuya Tateishi2 | Tsuyoshi Oda2 | Lin Cui1 | Kazutaka Kuramoto1 |  \nHideaki Yahata1 | Kaoru Okugawa1 | Shoji Maenohara1 | Hiroshi Yagi1 | Masafumi Yasunaga1 | Ichiro Onoyama1 | Kazuo Asanoma1 | Takeshi Mori2 | Yoshiki Katayama2,3 | Kiyoko Kato1  \n1Department of Obstetrics and Gynecology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan  \n2Department of Chemistry and Biochemistry, Graduate School of Systems Life Sciences, Graduate School of Engineering, Kyushu University, Fukuoka, Japan  \n3 Department of Biomedical Engineering, Chung Yuan Christian University, Taoyuan City, Taiwan  \nCorrespondence  \nKiyoko Kato, Department of Obstetricsand Gynecology, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.  \nEmail: [kato.kiyoko.172@m.kyushu-u.ac.jp](kato.kiyoko.172@m.kyushu-u.ac.jp)  \nFunding information  \nGrant-in-Aid for Scientific Research, Kyushu University School of Medicine Obstetrics and Gynecology Research Fund, Grant/Award Number:  \nFAKF401232; Japan Society for the Promotion of Science, Grant/Award Number: JP19K09804  \nAbstract  \nIndocyanine green (ICG) with near-infrared (NIR) fluorescence imaging is used for lymphatic mapping. However, binding of ICG to blood proteins like serum albumin can shorten its retention time in sentinel lymph nodes (SLNs) . Here, we investigated the efficacy and safety of a new fluorescence tracer comprising phytate and liposome (LP)-encapsulated ICG. Coadministration of phytate with LP containing phosphatidic acid promotes chelation mediated by Ca2+ in bodily fluids to enhance SLN retention. Uniformly sized LPs (100 nm) encapsulating ICG under conditions that minimized fluorescence self-quenching during storage were produced. We analyzed the behavior of the new tracer (ICG-phytate-LP) and control tracers (ICG and ICG-LP) in the lymphatic flow of mice in terms of lymph node retention time. We also tested lymphatic flow and safety in pigs that have a more human-like lymphatic system. LPs encapsulating stabilized ICG were successfully prepared. Mixing LP with phytate in the presence of Ca2+ increased both the particle size and negative surface charge. In mice, ICG-phytate-LP had the best lymph node retention, with a fluorescence intensity ratio that increased over 6 h and then decreased slowly over the next 24 h. In pigs, administration of ICG and ICG-phytate-LP resulted in no death or weight loss. There were no obvious differences between blood test results for the ICG and ICG-phytate-LP groups, and the overall safety was good. ICG-phytate-LP may be a useful new tracer for gynecological cancers that require time for lymph node identification due to a retroperitoneal approach.  \nK E Y WO R D S  \nindocyanine green, liposome, phytate, sentinel lymph node, tracer  \nAbbreviations: 99mTc, technetium-99m; DLS, dynamic light scattering; DMPA, dimyristoyl-sn-glycero-3-phosphatidic acid; HSPC, hydrogenated soybean phosphatidylcholine; ICG, indocyanine green; LP, liposome; NIR, near-infrared; PB, phosphate buffer; PdI, polydispersity index; PLN, pelvic lymph node; PLND, pelvic lymph node dissection; RI, radioisotope; SLN, sentinel lymph node; SLNB, sentinel lymph node biopsy; SN, secondary lymph node.  \nThis is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.  \n© 2023 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.  \n1 | INTRODUCTION  \nSentinel lymph nodes (SLNs) are the first lymph nodes to receive lymphatic inflow from a primary tumor, and sentinel lymph node biopsy (SLNB) is used to determine whether lymph nodes","cbCaihD93bDN6qrc","https://ap.wps.com/l/cbCaihD93bDN6qrc","pdf",2151310,"English","# Abstract\n## Introduction\n## Sentinel lymph nodes and SLNB\n## Clinical context in cervical cancer\n## Existing tracer approaches\n## Limits of indocyanine green fluorescence","[{\"question\":\"Why is enhancing sentinel lymph node retention important in cervical cancer imaging?\",\"answer\":\"SLN biopsy helps determine whether lymph nodes are metastatic, guiding the need for adjuvant therapy. Earlier metastasis detection is important, and avoiding unnecessary lymph node dissection can reduce complications such as lymphedema and lymphangitis.\"},{\"question\":\"How does the new tracer ICG-phytate-LP improve SLN retention?\",\"answer\":\"Coadministration of phytate with liposomes containing phosphatidic acid promotes Ca2+-mediated chelation in bodily fluids, improving SLN retention. Uniform ICG-loaded liposomes were produced while minimizing fluorescence self-quenching during storage.\"},{\"question\":\"What safety and efficacy results were observed in mice and pigs?\",\"answer\":\"In mice, ICG-phytate-LP provided the best lymph node retention, with fluorescence intensity increasing over 6 hours before slowly decreasing over the next 24 hours. In pigs, administration of ICG and ICG-phytate-LP caused no death or weight loss, and blood tests showed no obvious differences between groups, indicating good overall safety.\"}]","Development of novel tracers for sentinel node identification in cervical cancer | PDF",1790253890,23]