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A novel micro-computed tomography (micro-CT)-based three-dimensional scoring system was developed to quantify joint structure changes in a rat model of monoiodoacetate (MIA)-induced inflammatory knee OA. The system showed excellent interobserver reliability and strong correlation with a modified Mankin histological score.",{"@graph":69,"@context":123},[70,84,106],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/development-of-a-novel-micro-ct-based-scoring-system-for-the-assessment-of-joint-structure-in-a-rat-model-of-inflammatory-knee-osteoarthritis/441649/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/development-of-a-novel-micro-ct-based-scoring-system-for-the-assessment-of-joint-structure-in-a-rat-model-of-inflammatory-knee-osteoarthritis/441649.png","ImageObject",300,407,{"name":92,"@type":93},"Quinn Holloway","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-11-09","2026-09-29",true,{"@type":102,"interactionType":103,"userInteractionCount":105},"InteractionCounter",{"@type":104},"ViewAction",14,{"@type":107,"mainEntity":108},"FAQPage",[109,115,119],{"name":110,"@type":111,"acceptedAnswer":112},"Why is a new micro-CT scoring system needed for knee osteoarthritis research?","Question",{"text":113,"@type":114},"Traditional histology requires animal sacrifice, is time-consuming, and evaluates limited sections, making whole-joint assessment difficult. Micro-CT enables non-invasive 3D visualization but previously lacked detailed cartilage-focused assessment, motivating the new scoring system.","Answer",{"name":116,"@type":111,"acceptedAnswer":117},"How was osteoarthritis induced and scored in the rat model?",{"text":118,"@type":114},"Right knees of 24 Wistar male rats were injected with varying MIA concentrations (0.2, 0.5, 1, 3 mg). After 28 days, joints were scanned by micro-CT, segmented into 10 compartments, and each compartment was scored (0–4) to compute a total joint score.",{"name":120,"@type":111,"acceptedAnswer":121},"What evidence supports reliability and validity of the micro-CT scoring system?",{"text":122,"@type":114},"The micro-CT 3D imaging scoring system demonstrated excellent interobserver reliability with interclass correlation coefficients above 0.75 for most compartments. Total micro-CT joint scores showed a strong correlation with the modified Mankin histological score (ρ=0.8, P\u003C0.001).","https://schema.org",{"og:url":83,"og:type":125,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":127,"canonical":83},"index,follow",{"doc_id":129,"site_id":62},441649,1790720434,{"code":4,"msg":5,"data":132},{"doc_id":129,"user_id":133,"nickname":92,"user_avatar":134,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":135,"file_id":136,"file_url":137,"file_type":138,"file_size":139,"view_count":105,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":39,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":46,"preview_page_count":4},2336474466712,"https://ap-avatar.wpscdn.com/davatar_a8503ba1806abce46bf441b54a3ca4cd","Original Article | Basic and Mechanism Sciences   \nDevelopment of a novel micro-CT based scoring system for the assessment of joint structure in a rat model of inflammatory knee osteoarthritis  \nTakuya Takata1, Tatsunori Ikemoto1, Young-Chang Arai2, Yusuke Ohmichi3, Mika Ohmichi3, Kohei Hashimoto1, Masaki Nakano1, Nobunori Takahashi1^  \n1Department of Orthopedics, School of Medicine, Aichi Medical University, Nagakute, Japan; 2Department of Pain Medicine, Aichi Medical University, Nagakute, Japan; 3Department of Anatomy II, Kanazawa Medical University, Kahoku, Ishikawa, Japan  \nContributions: (I) Conception and design: T Takata, T Ikemoto, YC Arai; (II) Administrative support: Y Ohmichi, M Ohmichi, M Nakano, N Takahashi; (III) Provision of study materials or patients: T Ikemoto, YC Arai, Y Ohmichi, M Ohmichi; (IV) Collection and assembly of data: T Takata, T Ikemoto, YC Arai, K Hashimoto; (V) Data analysis and interpretation: T Takata, T Ikemoto, Y Ohmichi, M Ohmichi, K Hashimoto, M Nakano, N Takahashi; (VI) Manuscript writing: All authors; (VII) Final approval of manuscript: All authors.  \nCorrespondence to: Tatsunori Ikemoto, MD, PhD. Department of Orthopedics, School of Medicine, Aichi Medical University, 1-1 Yazako Karimata, Nagakute, Aichi, 480-1195, Japan. Email: [tatsunon31@gmail.com](tatsunon31@gmail.com).  \nBackground: Osteoarthritis (OA) is a degenerative joint disease involving cartilage loss and inflammation.  \nTraditional histological evaluation is limited. Micro-computed tomography (micro-CT) offers non-invasive three-dimensional (3D) joint visualization but lacks detailed cartilage assessment. This study developed and evaluated a novel micro-CT-based scoring system for joint structure in a rat model of mono-iodoacetate  \n(MIA)-induced OA.  \nMethods: OA was induced in the right knees of 24 Wistar male rats using varying MIA concentrations (0.2, 0.5, 1, 3 mg). After 28 days, knee joints were scanned using micro-CT and segmented into 10 compartments.  \nDegenerative changes in each compartment were scored (0–4), and a total joint score was calculated.  \nHistological evaluation using a modified Mankin score was performed on sagittal sections of the patellar groove. Interobserver reliability and correlations between micro-CT joint scores and Mankin scores were  \nanalyzed statistically.  \nResults: The micro-CT 3D imaging scoring system demonstrated excellent interobserver reliability [interclass correlation coefficient (ICC) >0.75 for most compartments] . Micro-CT joint scores revealed statistically significant differences in joint degeneration between the highest MIA dose group (3 mg) and the lower dose groups (0.2 and 0.5 mg) . Notably, micro-CT could differentiate between the 0.2 mg and 1mg groups, which was not fully reflected in histological scores. A strong correlation was found between the total  \nmicro-CT joint score and the modified Mankin score (ρ=0.8, P\u003C0.001) .  \nConclusions: The novel micro-CT-based 3D imaging scoring system provides a reliable method for quantifying gross degenerative changes in rat knee joints in the MIA-induced OA model. While it does not directly assess cartilage, its correlation with histological findings suggests its utility as a complementary tool  \nfor evaluating overall joint degeneration.  \nKeywords: Micro-computed tomography (micro-CT); knee osteoarthritis (knee OA); rat model; joint structure;  \nscoring system  \nSubmitted Jul 07, 2025. Accepted for publication Nov 04, 2025. Published online Dec 24, 2025.  \ndoi: 10.21037/atm-25-101  \nView this article at: [https://dx.doi.org/10.21037/atm-25-101](https://dx.doi.org/10.21037/atm-25-101)  \n^ ORCID: 0000-0002-4521-7639.  \n© AME Publishing Company. Ann Transl Med 2025;13(6):73 | [https://dx.doi.org/10.21037/atm-25-101](https://dx.doi.org/10.21037/atm-25-101)  \nPage 2 of 8 Takata et al. Micro-CT evaluation for rat knee OA  \nIntroduction  \nOsteoarthritis (OA) is a chronic degenerative disease characterized by the degeneration and loss ","cbCaiqaPnymQcc8P","https://ap.wps.com/l/cbCaiqaPnymQcc8P","pdf",1581810,"English","# Introduction\n# Methods\n# Results\n# Conclusions\n# Keywords","[{\"question\":\"Why is a new micro-CT scoring system needed for knee osteoarthritis research?\",\"answer\":\"Traditional histology requires animal sacrifice, is time-consuming, and evaluates limited sections, making whole-joint assessment difficult. Micro-CT enables non-invasive 3D visualization but previously lacked detailed cartilage-focused assessment, motivating the new scoring system.\"},{\"question\":\"How was osteoarthritis induced and scored in the rat model?\",\"answer\":\"Right knees of 24 Wistar male rats were injected with varying MIA concentrations (0.2, 0.5, 1, 3 mg). After 28 days, joints were scanned by micro-CT, segmented into 10 compartments, and each compartment was scored (0–4) to compute a total joint score.\"},{\"question\":\"What evidence supports reliability and validity of the micro-CT scoring system?\",\"answer\":\"The micro-CT 3D imaging scoring system demonstrated excellent interobserver reliability with interclass correlation coefficients above 0.75 for most compartments. Total micro-CT joint scores showed a strong correlation with the modified Mankin histological score (ρ=0.8, P\\u003c0.001).\"}]","Development of a novel micro-CT based scoring system for the assessment of joint structure in a rat model of inflammatory knee osteoarthritis | PDF",1790696942]