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The study combines RPA with CRISPR/Cas12a to enable sensitive, specific point-of-care detection in clinical samples using visual fluorescence readouts and lateral flow strip lateral flow sensors.",{"@graph":14,"@context":73},[15,34,56],{"@type":16,"itemListElement":17},"BreadcrumbList",[18,23,27,31],{"item":19,"name":20,"@type":21,"position":22},"https://docshare.wps.com","Home","ListItem",1,{"item":24,"name":25,"@type":21,"position":26},"https://docshare.wps.com/document/","Document",2,{"item":28,"name":29,"@type":21,"position":30},"https://docshare.wps.com/document/healthcare/","Healthcare",3,{"item":32,"name":10,"@type":21,"position":33},"https://docshare.wps.com/document/development-of-a-field-deployable-rpa-crisprcas12a-assay-for-the-detection-of-cyclospora-cayetanensis-in-human-feces/422607/",4,{"url":32,"name":10,"@type":35,"image":36,"author":41,"headline":10,"publisher":44,"fileFormat":47,"inLanguage":8,"description":12,"dateModified":48,"datePublished":49,"encodingFormat":47,"isAccessibleForFree":50,"interactionStatistic":51},"DigitalDocument",{"url":37,"@type":38,"width":39,"height":40},"https://docshare.wps.com/thumbnails/development-of-a-field-deployable-rpa-crisprcas12a-assay-for-the-detection-of-cyclospora-cayetanensis-in-human-feces/422607.png","ImageObject",300,407,{"name":42,"@type":43},"jay","Person",{"url":19,"name":45,"@type":46},"DocShare","Organization","application/pdf","2026-10-23","2026-09-28",true,{"@type":52,"interactionType":53,"userInteractionCount":55},"InteractionCounter",{"@type":54},"ViewAction",7,{"@type":57,"mainEntity":58},"FAQPage",[59,65,69],{"name":60,"@type":61,"acceptedAnswer":62},"What problem does the study target in Cyclospora detection?","Question",{"text":63,"@type":64},"The work addresses the lack of a sensitive immediate test that can enable rapid diagnosis of cyclosporiasis from human feces.","Answer",{"name":66,"@type":61,"acceptedAnswer":67},"How does the proposed assay detect Cyclospora cayetanensis?",{"text":68,"@type":64},"It combines recombinase polymerase amplification (RPA) with the CRISPR/Cas12a system, producing visual fluorescence readings under blue light and field detection via lateral flow strip (LFS) biosensors.",{"name":70,"@type":61,"acceptedAnswer":71},"What detection performance and validation results are reported?",{"text":72,"@type":64},"The assay shows a detection limit of 7 copies/µL and 30 oocysts per gram under simulated clinical conditions, distinguishes C. cayetanensis from related Eimeria spp., and matches nested PCR results for 30 clinical stool samples with three positives.","https://schema.org",{"og:url":32,"og:type":75,"og:title":10,"og:site_name":45,"og:description":12},"article",{"robots":77,"canonical":32},"index,follow",{"doc_id":79,"site_id":7},422607,1792216127,{"code":4,"msg":82,"data":83},"success",[84,88,92,96,101,106,109,114,119,122,126],{"id":22,"doc_module":4,"doc_module_name":25,"category_name":85,"show_sort_weight":86,"slug":87},"Story & Novel",90,"story-novel",{"id":26,"doc_module":4,"doc_module_name":25,"category_name":89,"show_sort_weight":90,"slug":91},"Literature",80,"literature",{"id":33,"doc_module":4,"doc_module_name":25,"category_name":93,"show_sort_weight":94,"slug":95},"Exam",70,"exam",{"id":97,"doc_module":4,"doc_module_name":25,"category_name":98,"show_sort_weight":99,"slug":100},5,"Comic",60,"comic",{"id":102,"doc_module":4,"doc_module_name":25,"category_name":103,"show_sort_weight":104,"slug":105},6,"Technology",50,"technology",{"id":55,"doc_module":4,"doc_module_name":25,"category_name":29,"show_sort_weight":107,"slug":108},40,"healthcare",{"id":110,"doc_module":4,"doc_module_name":25,"category_name":111,"show_sort_weight":112,"slug":113},8,"Research & Report",30,"research-report",{"id":115,"doc_module":4,"doc_module_name":25,"category_name":116,"show_sort_weight":117,"slug":118},9,"Religion & Spirituality",20,"religion-spirituality",{"id":117,"doc_module":4,"doc_module_name":25,"category_name":120,"show_sort_weight":117,"slug":121},"World Cup","world-cup",{"id":123,"doc_module":4,"doc_module_name":25,"category_name":124,"show_sort_weight":123,"slug":125},10,"Lifestyle","lifestyle",{"id":127,"doc_module":4,"doc_module_name":25,"category_name":128,"show_sort_weight":97,"slug":129},19,"General","general",{"code":4,"msg":82,"data":131},{"doc_id":79,"user_id":132,"nickname":42,"user_avatar":133,"doc_module":4,"category_id":55,"category_name":29,"doc_title":10,"doc_description":12,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":55,"is_deleted":4,"is_public":22,"is_downloadable":22,"audit_status":22,"page_count":139,"language":140,"language_code":8,"site_id":7,"html_lang":8,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":12,"update_tm":144,"read_time":145,"preview_page_count":4},3985747858866,"https://ap-avatar.wpscdn.com/davatar_a8503ba1806abce46bf441b54a3ca4cd","Qin et al. Parasites & Vectors (2026) 19:4 [https://doi.org/10.1186/s13071-025-07150-x](https://doi.org/10.1186/s13071-025-07150-x)  \nParasites & Vectors  \n RESEARCH Open Access  \nDevelopment of a field-deployable  \nRPA-CRISPR/Cas12a assay for the detection of Cyclospora cayetanensis in human feces  \nZiyang Qin1,2,3, Yilin Wang1,2,3, Mengqing Sun1,2,3, Qinglin Wang1,2,3, Junxia Duan 1,2,3, Chunhao Gu1,2,3, Xinfu Zhang1,2,3,4, Fuchang Yu5, Yayun Wu1,2,3, Huiyan Xu1,2,3, Junqiang Li1,2,3* and Longxian Zhang1,2,3*  \nAbstract  \nBackground Cyclospora is an emerging intestinal pathogenic protozoan transmitted through foodborne and waterborne routes. At least 19 countries in the world have recorded outbreaks of cyclosporiasis, mainly associated  \nwith the consumption of contaminated fresh agricultural products. The lack of a sensitive immediate test is oneof the major obstacles to the rapid diagnosis of cyclosporiasis. The target interference mechanisms of clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) protein systems have been adapted into versatile and efficient genome manipulation and disease-curing technologies, while also being promising for point-of-care testing (POCT) applications. It can serve as an excellent rapid and specific detection tool.  \nMethods The recombinase polymerase amplification (RPA) and the CRISPR/Cas12a system were combined to develop a detection method for C. cayetanensis (termed RECCT-Cay) via visual observation of fluorescent readings under blue light and field diagnosis using lateral flow strip (LFS) biosensors.  \nResults The detection limit of the established RECCT-Cay was 7 copies/μL. Under simulated clinical conditions, the detection limit was 30 oocysts per gram of stool. At the same time, the established detection platform can distinguish C. cayetanensis from the closely related Eimeria spp. The results of our constructed assay were compared with nested PCR, and the detection results of 30 clinical stool samples were consistent, with three samples positive for C. cayetanensis. Based on the RECCT-Cay detection principle, a portable suitcase-sized device has been designed, which can conduct rapid on-site detection of clinical samples.  \nConclusions The RECCT-Cay platform features rapid speed, high sensitivity, and the capability for field detection, making it a promising tool for use in remote areas.  \nKeywords Cyclospora cayetanensis, Recombinase polymerase amplification, CRISPR/Cas12a, Visualized detection, On-site detection  \n*Correspondence:  \nJunqiang Li[lijunqiangcool@126.com](lijunqiangcool@126.com)[ ](lijunqiangcool@126.com)Longxian Zhang [zhanglx8999@henau.edu.cn](zhanglx8999@henau.edu.cn)  \nFull list of author information is available at the end of the article  \n© The Author(s) 2025. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit [http://creativecommons.org/licenses/by/4.0/](http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver ()[. The Creative Commons Public Domain Dedication waiver (](http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver ()[http://creativeco](http://creativeco)[mmons.org/publicdomain/zero/1.0/]","cbCairxUKvJzHaXf","https://ap.wps.com/l/cbCairxUKvJzHaXf","pdf",2298435,14,"English","# Abstract\n## Background\n## Methods\n## Results\n## Conclusions","[{\"question\":\"What problem does the study target in Cyclospora detection?\",\"answer\":\"The work addresses the lack of a sensitive immediate test that can enable rapid diagnosis of cyclosporiasis from human feces.\"},{\"question\":\"How does the proposed assay detect Cyclospora cayetanensis?\",\"answer\":\"It combines recombinase polymerase amplification (RPA) with the CRISPR/Cas12a system, producing visual fluorescence readings under blue light and field detection via lateral flow strip (LFS) biosensors.\"},{\"question\":\"What detection performance and validation results are reported?\",\"answer\":\"The assay shows a detection limit of 7 copies/µL and 30 oocysts per gram under simulated clinical conditions, distinguishes C. cayetanensis from related Eimeria spp., and matches nested PCR results for 30 clinical stool samples with three positives.\"}]","Development of a field-deployable RPA-CRISPR/Cas12a assay for the detection of Cyclospora cayetanensis in human feces | PDF",1790628105,35]