[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-291022-105":59,"doc-detail-291022-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","developing-a-recipe-to-teach-elutions-to-blood-bank-students","Developing a Recipe to Teach Elutions to Blood Bank Students","","Hands-on practice of clinical laboratory testing requires real or simulated specimens, yet blood bank serological procedures like antibody elution are difficult to model. This study presents a practical in-vitro technique that sensitizes antibody onto large quantities of packed red blood cells, enabling multiple learners to elute and identify the antibody. Sensitization is monitored by DAT and optimized for appropriate IgG reactivity before learner elution and identification.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/developing-a-recipe-to-teach-elutions-to-blood-bank-students/291022/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/developing-a-recipe-to-teach-elutions-to-blood-bank-students/291022.png","ImageObject",300,407,{"name":92,"@type":93},"Anda","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-18","2026-09-17",true,{"@type":102,"interactionType":103,"userInteractionCount":44},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What problem does the study address for blood bank education?","Question",{"text":112,"@type":113},"It addresses the difficulty of simulating specimens for blood bank serological procedures such as antibody elution, which can limit hands-on practice.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How was the teaching sample prepared and optimized?",{"text":117,"@type":113},"Different volumes of monoclonal anti-D antisera were incubated with Rhesus-positive pRBCs, and optimum sensitization was defined by DAT reaction strength (≥2+) with IgG and a negative saline control.",{"name":119,"@type":110,"acceptedAnswer":120},"What did learners achieve using the method?",{"text":121,"@type":113},"Learners obtained positive DAT results for IgG and then successfully performed elution and identification of anti-D in the eluate, with outcomes matching those of an expert.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},291022,1789643190,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":44,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":8,"language":139,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":140,"faqs":141,"seo_title":142,"seo_description":67,"update_tm":129,"read_time":81,"preview_page_count":4},962075006959,"https://ap-avatar.wpscdn.com/avatar/e0002397efbe92a78e?_k=1776741047341049297","RESEARCH AND REPORTS  \nDeveloping a Recipe to Teach Elutions to Blood Bank Students  \nBRAD PFALTZGRAFF, LETYCIA NUNEZ-ARGOTE  \nABSTRACT  \nPROBLEM: Hands-on practice of clinical laboratory testing procedures with real or simulated specimens builds skill and competency. However, blood bank serological procedures, such as antibody elution, may be challenging to practice due to difficulty in simulating the nature of the specimen. This study exhibits a technique to sensitize antibody onto packed red blood cells (pRBCs) in vitro in large quantities so it can be eluted and identified by multiple learners.  \nMETHODS: Varying volumes of monoclonal anti-D antisera were incubated with a set volume of Rhesus-positive pRBCs. The direct antiglobulin test (DAT) was used to measure sensitization of the pRBCs. Optimum sensitization was considered a reaction strength of ≥2+ with immunoglobulin G (IgG) and a negative reaction for the saline control. A group of undergraduate clinical laboratory science students were given an aliquot from the optimally sensitized sample and instructed to perform a DAT with a reflex  \nto elution if positive. The elution procedure followed the Immucor Gamma ELU-KIT II package insert.  \nRESULTS: Learners successfully obtained a positive DAT for IgG and afterward eluted and identified anti-D in theeluate, matching the results obtained by the expert. Reaction strengths observed varied between 1–2+ in the eluate when run against panel cells.  \nCONCLUSION: By developing this teaching tool, we solved the problem of simulating DAT-positive samples and made it easier for learners to practice competency in elution techniques. This method can be applied to clinical and education laboratories, allowing for technical skill building and clinical competency maintenance in students and active laboratory professionals.  \nABBREVIATIONS: DAT - direct antiglobulin test, IgG immunoglobulin G, pRBC-packed red blood cell.  \nClin Lab Sci 2020;33(4):68  \nBrad Pfaltzgraff, The University of Kansas Medical Center, Kansas City, Kansas  \nLetycia Nunez-Argote, The University of Kansas Medical Center, Kansas City, Kansas  \nAddress for Correspondence: Brad Pfaltzgraff, The University of Kansas Medical Center, Kansas City, Kansas, bpfaltzgraff@[kumc.edu](kumc.edu)  \n68 | VOL 33, NO 4, FALL 2020, CLINICAL LABORATORY SCIENCE  \nwl afrom [http://hwmaint.clsjournal.ascls.org/](http://hwmaint.clsjournal.ascls.org/ Se)[ Se](http://hwmaint.clsjournal.ascls.org/ Se) e b 17 2026","cbCaiiI66DVrB2FW","https://ap.wps.com/l/cbCaiiI66DVrB2FW","pdf",42897,"English","# Abstract\n## Problem\n## Methods\n## Results\n## Conclusion\n# Abbreviations","[{\"question\":\"What problem does the study address for blood bank education?\",\"answer\":\"It addresses the difficulty of simulating specimens for blood bank serological procedures such as antibody elution, which can limit hands-on practice.\"},{\"question\":\"How was the teaching sample prepared and optimized?\",\"answer\":\"Different volumes of monoclonal anti-D antisera were incubated with Rhesus-positive pRBCs, and optimum sensitization was defined by DAT reaction strength (≥2+) with IgG and a negative saline control.\"},{\"question\":\"What did learners achieve using the method?\",\"answer\":\"Learners obtained positive DAT results for IgG and then successfully performed elution and identification of anti-D in the eluate, with outcomes matching those of an expert.\"}]","Developing a Recipe to Teach Elutions to Blood Bank Students | PDF"]