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Differentially expressed genes were identified from the GSE140797 dataset, followed by prognostic assessment using univariate and multivariate Cox regression and LASSO Cox modeling. Expression of ACAT1 and ACSL3 was validated by qRT-PCR and linked to immune scores, checkpoints, methylation, OCLR, and ferroptosis.",{"@graph":14,"@context":73},[15,34,56],{"@type":16,"itemListElement":17},"BreadcrumbList",[18,23,27,31],{"item":19,"name":20,"@type":21,"position":22},"https://docshare.wps.com","Home","ListItem",1,{"item":24,"name":25,"@type":21,"position":26},"https://docshare.wps.com/document/","Document",2,{"item":28,"name":29,"@type":21,"position":30},"https://docshare.wps.com/document/healthcare/","Healthcare",3,{"item":32,"name":10,"@type":21,"position":33},"https://docshare.wps.com/document/detection-of-the-fatty-acid-metabolism-linked-genes-in-lung-adenocarcinoma-original-article-biomarkers-and-immunotherapeutic-targets/336777/",4,{"url":32,"name":10,"@type":35,"image":36,"author":41,"headline":10,"publisher":44,"fileFormat":47,"inLanguage":8,"description":12,"dateModified":48,"datePublished":49,"encodingFormat":47,"isAccessibleForFree":50,"interactionStatistic":51},"DigitalDocument",{"url":37,"@type":38,"width":39,"height":40},"https://docshare.wps.com/thumbnails/detection-of-the-fatty-acid-metabolism-linked-genes-in-lung-adenocarcinoma-original-article-biomarkers-and-immunotherapeutic-targets/336777.png","ImageObject",300,407,{"name":42,"@type":43},"Riley","Person",{"url":19,"name":45,"@type":46},"DocShare","Organization","application/pdf","2026-10-28","2026-09-21",true,{"@type":52,"interactionType":53,"userInteractionCount":55},"InteractionCounter",{"@type":54},"ViewAction",6,{"@type":57,"mainEntity":58},"FAQPage",[59,65,69],{"name":60,"@type":61,"acceptedAnswer":62},"Which 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  \nDetection of the Fatty Acid Metabolism-Linked Genes in Lung Adenocarcinoma as Biomarkers for Clinical Prognosis and Immunotherapeutic Targets  \nJingwei Shi1 | Rusong Yang1 | Xinyi Jiang2 | Kangle Zhu3  | Zhengcheng Liu1   \n1Department of Thoracic Surgery, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, Jiangsu Province, China | 2Department of Cardiovascular and Thoracic Surgery, Nanjing Drum Tower Hospital, Chinese Academy of Medical Science & Peking Union Medical College, Nanjing University, Nanjing, Jiangsu Province, China | 3Department of Thoracic Surgery, Nanjing Drum Tower Hospital Clinical College  \nof Nanjing Medical University, Nanjing, Jiangsu Province, China Correspondence: Zhengcheng Liu ([zhengcheng.liu@njglyy.com](zhengcheng.liu@njglyy.com))  \nReceived: 1 March 2024 | Revised: 24 August 2024 | Accepted: 4 September 2024  \nFunding: This work was supported by Key Project of Nanjing Medical Science and Technology Program, ZKX21015 .  \nKeywords: ACAT1 | ACSL3 | biomarker | fatty acid metabolism | lung adenocarcinoma | qRT-PCR  \nABSTRACT  \nBackground: Lung cancer, on a global scale, leads to the most common cases of cancer mortalities. Novel therapeutic approaches are urgently needed to disrupt this lethal disease. The rapid development of tumor immunology combining breakthroughs involving fatty acid metabolism brings possibilities. Directing fatty acid metabolism is supposed to help discover potential prognostic biomarkers and treatment targets for lung cancer.  \nMethods: Through searching the GSE140797 dataset, we identified genes related to fatty acid metabolism as well as fatty acid metabolism-related differentially expressed genes (DEGs) . We applied various methods to ascertain the independent prognostic value of the DEGs. The methods we utilized entail prognostic analysis, differential expression analysis, as well as univariate and multivariate Cox regression analyses. The lasso Cox regression model was utilized in examining how DEGs correlate with the immune score, immune checkpoint, ferroptosis, methylation, and OCLR score. The expression levels of ACAT1 and ACSL3 in tissues derived from normal lung and lung adenocarcinoma (LUAD) tissues were compared by qRT-PCR.  \nResults: In this study, ACSL3 and ACAT1 were identified as fatty acid metabolism-related genes utilizing independent prognostic value and as a result, the risk prognostic model was built using these factors. qRT-PCR results implied that ACSL3 and ACAT1 expressions were upregulated and downregulated, correspondingly in tumor tissues. Additional evaluations suggested that ACSL3 and ACAT1 were affirmed to be remarkably correlated with the immune score, methylation, immune checkpoint, OCLR score, and ferroptosis.  \nConclusions: ACSL3 and ACAT1 were effective prognostic biomarkers and potential immunotherapeutic targets in LUAD.  \n\n| Abbreviations: ACAT1, Acetyl-CoA acetyltransferase 1; ACSL3, Acyl-CoA synthetase long chain family member 3; AUC, area under curve; CHB, chronic HBV; DEGs, differential expressed genes; FAs, fatty acids; FAS, fatty acid synthase; GO, gene ontology; GSEA, Gene Set Enrichment Analysis; HR, risk ratio; KEEG, Kyoto Encyclopedia of Genes and Genomes; LUAD, lung adenocarcinoma; OCLR, one-class logistic regression; OS, overall survival; PCA, principal-component analysis; RCCC, renal clear cell carcinoma; ROC, receiver operating characteristic curve; t-SNE, t-distributed stochastic neighbor embedding; m6A, N6-methyladenosine; NSCLC, non-small cell lung cancer; TME, tumor microenvironment. |\n| --- |\n| This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.\u003Cbr>© 2024 The Author(s) . The Clinical Respiratory Journal published by John Wiley & Sons Ltd. |\n\nThe Clinical Respirator","cbCaim8XnfTzeh2t","https://ap.wps.com/l/cbCaim8XnfTzeh2t","pdf",6467298,12,"English","# Abstract\n# Introduction","[{\"question\":\"Which data source was used to identify fatty-acid metabolism-related genes in LUAD?\",\"answer\":\"The study searched the GSE140797 dataset to identify fatty-acid metabolism-related genes and fatty-acid metabolism-related differentially expressed genes (DEGs).\"},{\"question\":\"How were the prognostic values of the DEGs evaluated?\",\"answer\":\"Independent prognostic value was assessed using prognostic analysis and univariate and multivariate Cox regression, with a LASSO Cox regression model used to build a risk model.\"},{\"question\":\"What did qRT-PCR validation show for ACAT1 and ACSL3 expression?\",\"answer\":\"qRT-PCR results indicated that ACAT1 and ACSL3 were dysregulated in tumor tissues compared with normal lung tissues, with ACSL3 and ACAT1 showing the reported up- and downregulation patterns.\"}]","Detection of the Fatty Acid Metabolism-Linked Genes in Lung Adenocarcinoma - Original Article - Biomarkers and Immunotherapeutic Targets | PDF",1790016573]