[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-366289-105":59,"doc-detail-366289-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","decreased-green-autofluorescence-in-cancerous-tissues-is-a-potential-biomarker-for-diagnosis-of-renal-cell-carcinomas-research","Decreased green autofluorescence in cancerous tissues is a potential biomarker for diagnosis of renal cell carcinomas - Research","","Study investigates reduced green autofluorescence in renal cell carcinoma (RCC) tissues and identifies cytokeratin sources responsible for the signal difference between cancerous and adjacent non-tumor tissues. Green autofluorescence under 488 nm laser excitation is compared between RCC and neighboring nontumor samples, while KRT1/KRT7 knockdown evaluates effects on intensity. Diagnostic performance is assessed using ROC analysis on 174 paired samples, yielding an AUC of 0.880 and supporting a threshold-based diagnostic model for real-time surgical margin assessment.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/decreased-green-autofluorescence-in-cancerous-tissues-is-a-potential-biomarker-for-diagnosis-of-renal-cell-carcinomas-research/366289/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/decreased-green-autofluorescence-in-cancerous-tissues-is-a-potential-biomarker-for-diagnosis-of-renal-cell-carcinomas-research/366289.png","ImageObject",300,407,{"name":92,"@type":93},"Adam","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-25","2026-09-23",true,{"@type":102,"interactionType":103,"userInteractionCount":29},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What does the study evaluate regarding green autofluorescence in RCC?","Question",{"text":112,"@type":113},"It evaluates why green autofluorescence intensity is decreased in cancerous RCC tissues compared with adjacent non-tumor tissues, focusing on keratins (KRT1, KRT7, KRT8).","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How was the effect of KRT1/KRT7 on autofluorescence intensity tested?",{"text":117,"@type":113},"The study analyzed autofluorescence changes after knocking down the KRT1/KRT7 gene and compared the resulting intensity with control conditions under 488 nm excitation.",{"name":119,"@type":110,"acceptedAnswer":120},"What diagnostic performance did ROC analysis show for tumor autofluorescence intensity?",{"text":121,"@type":113},"ROC analysis on 174 paired tumor and adjacent non-tumor samples produced an AUC of 0.880, with sensitivity of 0.843 and specificity of 0.835, using a threshold value of 27.45.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},366289,1790743101,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":29,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":44,"language":139,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":140,"faqs":141,"seo_title":142,"seo_description":67,"update_tm":143,"read_time":144},1374404737137,"https://ap-avatar.wpscdn.com/davatar_155a257f0dc6eb9ab79c44ca47cae57d","[www. nature.com/scientificreports](www. nature.com/scientificreports)  \nOPEN  \nDecreased green autofluorescence in cancerous tissues is a potential biomarker for diagnosis of renal cell carcinomas  \nWei Wan1,5, Junrong Zou2,3,5, Tianpeng Xie2,3,5, Liang Zeng1, Huiquan Liu1, Bo Jiang2,3, Yunfeng Liao2,3, Yuting Wu2,3, Gengqing Wu2,3, Guoxi Zhang2,3, Weihai Ying4􀀍 & Xiaofeng Zou1,2,3􀀍  \nThis study primarily focuses on the potential sources of autofluorescence, including keratins  \n(KRT) encoded by KRT1, KRT7, and KRT8, to investigate their contributions to the differences in autofluorescence between cancerous tissues and adjacent non-tumor tissues, as well as their potential for real-time diagnosis of RCC. First, the autofluorescence of renal cell carcinoma (RCC) tissues under 488 nm laser excitation was observed and compared with the autofluorescence of neighboring nontumor tissues. Then, the effect on the autofluorescence intensity was analyzed by knocking down the KRT1/KRT7 gene. In addition, autofluorescence data were collected from 174 pairs of tumor and adjacent non-tumor tissue samples (from 60 RCC patients). Diagnostic performance was evaluated using ROC analysis to determine the threshold value for tumor autofluorescence intensity. Under  \n488 nm laser excitation, the intensity of green autofluorescence in cancerous tissues of RCC patients was significantly lower than that in non-tumor tissues. Further analysis showed that KRT1 knockdown resulted in a 73% reduction in autofluorescence intensity, suggesting that KRT1 plays a key role in the reduced autofluorescence observed in tumor tissues. In addition, analysis of autofluorescence data from 174 tumor and adjacent non-tumor tissue samples showed anAUC of 0.880 for ROC analysis, a diagnostic sensitivity and specificity of 0.843 and 0.835, respectively, and a threshold value of 27.45 for using tumor autofluorescence intensity. KRT1 is a major contributor to the tumor autofluorescence observed in RCC. An autofluorescence-based diagnostic model facilitates real-time assessment of surgical margins during partial nephrectomy, thereby potentially improving surgical success rates. Keywords Autofluorescence, Diagnosis, Renal cell carcinoma, Cytokeratin-1  \nRenal cell carcinoma (RCC) originates from renal tubular epithelial cells and accounts for more than 90% of renal malignancies, with a mortality rate of approximately 30-40%1,2. The major histological subtypes of RCCis clear cell renal cell carcinoma, which accounts for 2% of all malignancies3. RCC is mostly asymptomatic in its early stages, and patients are often in a progressive stage when they are diagnosed. Radical nephrectomy and partial nephrectomy (PN) with preservation of nephrons are effective treatments for RCC (especially limited RCC)4. However, determination of tumor boundaries during performing PN is a major challenge5. Therefore, exploring simple, real-time and accurate diagnostic methods for RCC is of great significance for improving the prognosis.  \nAutofluorescence technology was based on utilizing bio-macromolecules that can be excited to fluoresce in vivo. In the past decade, which was demonstrated to be a promising tool for the rapid diagnosis of advanced cancers, including gastric cancer, lung cancer, and basal cell carcinoma etc6–8. Our previous study found that green autofluorescence at a wavelength of 500–550 nm, excited by a 488 nm laser, exhibited significant decreased autofluorescence in lung cancer7. Later study revealed that the source responsible for autofluorescence mainly came from keratin 1 (KRT1)9. As an epithelial neoplasm, RCC is characterized by widespread cytokeratin  \n1First Clinical Medical College, Gannan Medical University, Ganzhou, China. 2Institute of Urology, First Affiliated Hospital of Gannan Medical University, Ganzhou, China. 3Department of Urology, First Affiliated Hospital of Gannan Medical University, Ganzhou, China. 4Med-X Research Institute, School of Biomedical Engineerin","cbCait5UpwpjE75t","https://ap.wps.com/l/cbCait5UpwpjE75t","pdf",2119444,"English","# Materials and methods\n## Patients and ethical statement","[{\"question\":\"What does the study evaluate regarding green autofluorescence in RCC?\",\"answer\":\"It evaluates why green autofluorescence intensity is decreased in cancerous RCC tissues compared with adjacent non-tumor tissues, focusing on keratins (KRT1, KRT7, KRT8).\"},{\"question\":\"How was the effect of KRT1/KRT7 on autofluorescence intensity tested?\",\"answer\":\"The study analyzed autofluorescence changes after knocking down the KRT1/KRT7 gene and compared the resulting intensity with control conditions under 488 nm excitation.\"},{\"question\":\"What diagnostic performance did ROC analysis show for tumor autofluorescence intensity?\",\"answer\":\"ROC analysis on 174 paired tumor and adjacent non-tumor samples produced an AUC of 0.880, with sensitivity of 0.843 and specificity of 0.835, using a threshold value of 27.45.\"}]","Decreased green autofluorescence in cancerous tissues is a potential biomarker for diagnosis of renal cell carcinomas - Research | PDF",1790164610,23]