[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-349457-105":59,"doc-detail-349457-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","deciphering-transcriptional-plasticity-in-pancreatic-ductal-adenocarcinoma-reveals-alterations-in-sensory-neuron-innervation","Deciphering transcriptional plasticity in pancreatic ductal adenocarcinoma reveals alterations in sensory neuron innervation","","Neuronal innervation of the pancreas has been incompletely mapped at the transcriptomic level, limiting understanding of how neural remodeling affects pancreatic ductal adenocarcinoma (PDAC) progression and pain signaling. This study uses Fast Blue retrotracing in wild-type and PDAC mouse models, followed by single-cell RNA sequencing of sensory neurons from the pancreatic head and tail, validated by immunofluorescence, organoid cultures, and qPCR. Neurofilament-containing neurons dominate in both contexts, while non-peptidergic neurons are reduced in tumor-associated innervation.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/deciphering-transcriptional-plasticity-in-pancreatic-ductal-adenocarcinoma-reveals-alterations-in-sensory-neuron-innervation/349457/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/deciphering-transcriptional-plasticity-in-pancreatic-ductal-adenocarcinoma-reveals-alterations-in-sensory-neuron-innervation/349457.png","ImageObject",300,407,{"name":92,"@type":93},"Fans","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-09-25","2026-09-22",true,{"@type":102,"interactionType":103,"userInteractionCount":14},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"How were pancreatic sensory neurons identified in the study?","Question",{"text":112,"@type":113},"Wild-type and PDAC mice received Fast Blue retrotracer injections into pancreatic or cancerous tissue. Dorsal root ganglia were dissociated, and Fast Blue-positive sensory neurons were isolated for analysis.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"Which sensory neuron subtype predominated under normal and tumor conditions?",{"text":117,"@type":113},"Neurofilament-containing neurons were identified as the predominant sensory subtype in both normal and PDAC-associated innervation.",{"name":119,"@type":110,"acceptedAnswer":120},"What evidence supports a mechanism of tumor–neuron interaction?",{"text":121,"@type":113},"The study found transcripts transferred via extracellular vesicles, including those linked to the Pdx1-CreERT2 transgene in the KPC model, suggesting extracellular vesicle–mediated RNA transfer that could remodel sensory signaling.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},349457,1790334396,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":14,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":36},5909892330395,"https://ap-avatar.wpscdn.com/davatar_6f874abed73319feea01a86fa6f0fab8","Deciphering transcriptional plasticity in pancreatic ductaladenocarcinoma reveals alterations in sensory neuron innervation  \nElena Genova 1 , Michele Montrone2, Uday Rangaswamy2, Francesco Diversi2, Irene Schiavo2, Denise Ferrarini2, Roberta Di Florio2 , Irene Longo2, Michele Coscia2, Nicola Zamboni2, Giorgia Demontis2, Lisa Veghini3, Vincenzo Corbo3, Remo Sanges2 and Paul Heppenstall2  \n1 Institute for Maternal and Child Health- IRCCS “Burlo Garofolo”, Trieste, Italy  \n2 Scuola Internazionale Superiore di Studi Avanzati, Trieste, Italy  \n3 Department of Engineering for Innovation Medicine, University of Verona, Italy  \nKeywords  \npancreas; pancreatic ductaladenocarcinoma; sensory neurons; singlecell RNA sequencing; tumor microenvironment  \nCorrespondence  \nP. Heppenstall, Scuola Internazionale Superiore di Studi Avanzati, 34136, Trieste, Italy  \nE-mail: [pheppens@sissa.it](pheppens@sissa.it)[ ](pheppens@sissa.it)and  \nE. Genova, IRCCS Burlo Garofolo, 34137, Trieste, Italy  \nE-mail: [elena.genova@burlo.trieste.it](elena.genova@burlo.trieste.it)  \nElena Genova, Michele Montrone, and Uday Rangaswamy contributed equally to this work.  \n(Received 4 November 2025, revised 22 January 2026, accepted 20 February 2026, available online 18 March 2026)  \ndoi:10 . 1002/1878-0261 .70233  \nNeuronal innervation of the pancreas has historically been characterized using marker-based classification and physiological studies, but its transcriptomic landscape remains only partially explored. A detailed molecular profile of pancreatic sensory neurons could provide insights into their role in health and disease, particularly in pancreatic ductal adenocarcinoma (PDAC), where neural remodeling influences tumor progression and pain signaling. Wild-type and PDAC mice were injected with the retrotracer Fast Blue into pancreatic or cancerous tissue. Dorsal root ganglia were dissociated, and Fast Blue-positive sensory neurons were isolated, lysed, and analyzed using single-cell RNA sequencing. Data were validated using immunofluorescence, organoid cultures and qPCR. We performed transcriptomic profiling of sensory neurons innervating the pancreatic head and tail under normal and cancer conditions. Our analysis identified neurofilament-containing neurons asthe predominant sensory subtype in both contexts, while non-peptidergic neurons were underrepresented in tumor-associated innervation. Differential gene expression analysis revealed a unique subset of genes upregulated in sensory neurons innervating pancreatic tumors, many linked to mitochondrial activity. Further validation also revealed the presence of transcripts transferred via extracellular vesicles (including the Pdx1-CreERT2 transgene from the KPC mouse model), suggesting a novel mechanism of tumor– neuron interaction. Our findings provide a detailed characterization of pancreatic and pancreatic ductal adenocarcinoma sensory innervation. We identified tumor-derived RNA within sensory neurons in the PDAC mouse model, suggesting an extracellular vesicle–mediated RNA transfer mechanism that may remodel sensory signaling and open new prospects for diagnostic and therapeutic innovation in PDAC.  \nImpact statement  \nTranscriptomic profiling of pancreatic sensory neurons reveals shifts in neuronal populations, tumor-specific mitochondrial gene upregulation, and potential extracellular vesicle–mediated transcript transfer. Circulating  \nAbbreviations  \n50 UTR, untranslated region; DRG, dorsal root ganglia; EVs, extracellular vesicles; FB, Fast Blue; IF, immunofluorescence; KPC, Kras TrP53 Cre; LncRNA, long non-coding RNA; NF, neurofilament containing; NP, non-peptidergic; PDAC, pancreatic ductal adenocarcinoma; PEP, peptidergic; ScRNA-seq, single-cell RNA sequencing; TH, tyrosine hydroxylase; WT, wild type.  \n1726 Molecular Oncology 20 (2026) 1726–1741 ª 2026 The Author(s) . Molecular Oncology published by John Wiley & Sons Ltd on behalf of  \nFederation of European Biochemical Societies.  \nThis is an open access arti","cbCaijzeAoiHPPna","https://ap.wps.com/l/cbCaijzeAoiHPPna","pdf",1531050,16,"English","# Introduction\n## Neural circuits in pancreas homeostasis\n## Neural remodeling in cancer and PDAC\n# Methods\n## Fast Blue retrotracing and sensory neuron isolation\n## Single-cell RNA sequencing and validation\n# Results\n## Sensory neuron subtype shifts under normal vs PDAC conditions\n## Tumor-specific mitochondrial gene upregulation\n## Extracellular vesicle–mediated transcript transfer","[{\"question\":\"How were pancreatic sensory neurons identified in the study?\",\"answer\":\"Wild-type and PDAC mice received Fast Blue retrotracer injections into pancreatic or cancerous tissue. Dorsal root ganglia were dissociated, and Fast Blue-positive sensory neurons were isolated for analysis.\"},{\"question\":\"Which sensory neuron subtype predominated under normal and tumor conditions?\",\"answer\":\"Neurofilament-containing neurons were identified as the predominant sensory subtype in both normal and PDAC-associated innervation.\"},{\"question\":\"What evidence supports a mechanism of tumor–neuron interaction?\",\"answer\":\"The study found transcripts transferred via extracellular vesicles, including those linked to the Pdx1-CreERT2 transgene in the KPC model, suggesting extracellular vesicle–mediated RNA transfer that could remodel sensory signaling.\"}]","Deciphering transcriptional plasticity in pancreatic ductal adenocarcinoma reveals alterations in sensory neuron innervation | PDF",1790083399]