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This study investigates how curcumin affects adipose-derived mesenchymal stem cells (ADSCs) and whether curcumin-pretreated ADSCs improve OA outcomes. Curcumin reduces ROS and suppresses ADSC senescence, while enhancing FoxO3-dependent autophagy by regulating Becn1. In vivo, curcumin-pretreated ADSCs improve cartilage degeneration, synovitis, and subchondral bone deterioration and support joint homeostasis.",{"@graph":69,"@context":123},[70,84,106],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/curcumin-controls-the-senescence-of-adipose-derived-mesenchymal-stem-cells-via-the-foxo3autophagy-signaling-pathway-osteoarthritis-therapy/442330/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/curcumin-controls-the-senescence-of-adipose-derived-mesenchymal-stem-cells-via-the-foxo3autophagy-signaling-pathway-osteoarthritis-therapy/442330.png","ImageObject",300,407,{"name":92,"@type":93},"Kurz","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-11-09","2026-09-29",true,{"@type":102,"interactionType":103,"userInteractionCount":105},"InteractionCounter",{"@type":104},"ViewAction",17,{"@type":107,"mainEntity":108},"FAQPage",[109,115,119],{"name":110,"@type":111,"acceptedAnswer":112},"What problem does this study address in osteoarthritis therapy?","Question",{"text":113,"@type":114},"OA involves whole-joint pathology and is limited by senescence of mesenchymal stem cells under aging or stress, reducing therapeutic efficacy. 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When FoxO3 is knocked down with siRNA, autophagy and senescence restoration in ADSCs are impaired.","https://schema.org",{"og:url":83,"og:type":125,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":127,"canonical":83},"index,follow",{"doc_id":129,"site_id":62},442330,1790872148,{"code":4,"msg":5,"data":132},{"doc_id":129,"user_id":133,"nickname":92,"user_avatar":134,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":135,"file_id":136,"file_url":137,"file_type":138,"file_size":139,"view_count":105,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":140,"language":141,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":142,"faqs":143,"seo_title":144,"seo_description":67,"update_tm":145,"read_time":36,"preview_page_count":4},2336478945635,"https://ap-avatar.wpscdn.com/davatar_6f874abed73319feea01a86fa6f0fab8","Zhang et al. Stem Cell Research & Therapy (2026) 17:7 [https://doi.org/10.1186/s13287-025-04836-y](https://doi.org/10.1186/s13287-025-04836-y)  \nStem Cell Research & Therapy  \nRESEARCH Open Access  \nCurcumin controls the senescence of adipose-derived mesenchymal stem cells via the FoxO3/autophagy signaling pathway to enhance joint homeostasis for osteoarthritis therapy  \nRui Zhang 1,2, Lei Huang 1, Meng Jin 1, Haiyang Kou 1, Jie Ma3, Weijie Zhang 1* and Jianbing Ma 1*  \nAbstract  \nBackground Osteoarthritis (OA) is a common chronic and degenerative disease, the pathophysiological features of which involve the whole joint. In recent years, adipose tissue-derived mesenchymal stem cells (ADSCs) have attracted much attention in the field of regenerative medicine. Nonetheless, with aging in vivo or exogenous stress in vitro, mesenchymal stem cells undergo cellular senescence, resulting in limited therapeutic efficacy. Curcumin is a natural polyphenolic compound that has antioxidative, anti-inflammatory and antiapoptotic properties. We aimed to investigate the function and molecular mechanisms ofthe effects of curcumin on ADSC senescence and to explore the therapeutic efficacy of curcumin-pretreated ADSCs in OA management.  \nMethods Mouse primary ADSCs were identified using immunofluorescence staining and multilineage differentiation. Cell counting kit-8 (CCK-8) assays, reactive oxygen species (ROS) level measurements, β-galactosidase staining, Western blotting, immunofluorescence staining, and transmission electron microscopy (TEM) were performed to explore the function of curcumin in ADSCs. Bioinformatics analysis, chromatin immunoprecipitation (ChIP), small interfering RNA (siRNA), and real-time quantitative PCR (RT‒qPCR) were performed to evaluate whether FoxO3 directly regulates Becn1 . In vivo, adult C57BL/6 male mice were subjected to destabilization of the medial meniscus (DMM) to establish an OA model. Histological staining with HE and Safranin O and fast green, Osteoarthritis Research Society International (OARSI) and synovitis score evaluation, histochemical staining, and microcomputed tomography (µCT) analysis were performed to assess the effects of intra-articular injection of ADSCs on OA treatment. Results Curcumin inhibited ROS production and mitigated ADSC senescence. Furthermore, curcumin promoted autophagy and subsequent alleviation of cell senescence via a FoxO3-dependent pathway, and FoxO3 directly regulated the autophagy-related gene Becn1 . Knockdown of FoxO3 with small interfering RNA (siRNA) impaired  \n*Correspondence: Weijie Zhang [zitengbiyue@163.com](zitengbiyue@163.com)[ ](zitengbiyue@163.com)Jianbing Ma [13299028888@163.com](13299028888@163.com)  \nFull list of author information is available at the end of the article  \n© The Author(s) 2025. Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit [http://creati](http://creati)[vecommons.org/l](vecommons.org/l)icenses/by-nc-nd/4.0/.  \nZhang et al. Stem Cell Research & Therapy (2026) 17:7 Page 2 of 16  \nautophagy and restored cell senescence in ADSCs. In vivo,","cbCaioRaaC4BK3ay","https://ap.wps.com/l/cbCaioRaaC4BK3ay","pdf",10411584,16,"English","# Abstract\n## Background\n## Methods\n## Results\n## Conclusions\n# Keywords\n# Introduction","[{\"question\":\"What problem does this study address in osteoarthritis therapy?\",\"answer\":\"OA involves whole-joint pathology and is limited by senescence of mesenchymal stem cells under aging or stress, reducing therapeutic efficacy. The study targets ADSC senescence as a key barrier to effective treatment.\"},{\"question\":\"How does curcumin affect ADSCs in the experiments?\",\"answer\":\"Curcumin inhibits reactive oxygen species (ROS) production and mitigates ADSC senescence. It also promotes autophagy and alleviates senescence through a FoxO3-dependent pathway.\"},{\"question\":\"What is the role of FoxO3 and Becn1 in the mechanism?\",\"answer\":\"The study reports that FoxO3 promotes autophagy by directly regulating the autophagy-related gene Becn1. When FoxO3 is knocked down with siRNA, autophagy and senescence restoration in ADSCs are impaired.\"}]","Curcumin Controls the Senescence of Adipose-Derived Mesenchymal Stem Cells via the FoxO3/Autophagy Signaling Pathway - Osteoarthritis Therapy | PDF",1790699863]