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Immunohistochemistry was performed on 60 breast cancer specimens, followed by transcriptomic atlas analysis of 1533 patients to connect c-Myc with cuproptosis and stemness.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/cuproptosis-engages-in-c-myc-mediated-breast-cancer-stemness-research-open-access/383729/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/cuproptosis-engages-in-c-myc-mediated-breast-cancer-stemness-research-open-access/383729.png","ImageObject",300,407,{"name":92,"@type":93},"Rhys","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-09-26","2026-09-24",true,{"@type":102,"interactionType":103,"userInteractionCount":14},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What is the main relationship investigated between c-Myc and breast cancer stemness?","Question",{"text":112,"@type":113},"The study finds a positive correlation between c-Myc and the breast cancer stem cell (BCSC) phenotype. Transcriptomic analysis links c-Myc, cancer stemness, and cuproptosis-related mechanisms.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How were the experimental and analytical datasets used in the study?",{"text":117,"@type":113},"Immunohistochemical staining was performed on 60 breast cancer surgical specimens to assess markers related to stemness and c-Myc. Separately, transcriptomic data from 1533 breast cancer patients were analyzed from public databases.",{"name":119,"@type":110,"acceptedAnswer":120},"What does the study report about cuproptosis-related features and clinical outcomes?",{"text":121,"@type":113},"Cuproptosis-related characteristics were screened that predict poor clinical outcomes. The pro-tumoral cuproptosis-based features were enriched in MYC-targets and correlated positively with cancer stemness.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},383729,1790397213,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":14,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":145},687207024643,"https://ap-avatar.wpscdn.com/davatar_3d24733baf745e90a7e4bdd5f77d97b2","Wang et al. Journal of Translational Medicine (2023) 21:409  \n[https://doi.org/10.1186/s12967-023-04204-5](https://doi.org/10.1186/s12967-023-04204-5)  \nJournal of Translational Medicine  \n RESEARCH Open Access  \nCuproptosis engages in c-Myc-mediated breast cancer stemness  \nRuntian Wang1†, Kun Xu1†, Qin Chen1†, Qin Hu2†, Jian Zhang3* and Xiaoxiang Guan1,4*  \nAbstract  \nBackground Intra-tumoral heterogeneity (ITH) is a distinguished hallmark of cancer, and cancer stem cells (CSCs) contribute to this malignant characteristic. Therefore, it is of great significance to investigate and even target the regulatory factors driving intra-tumoral stemness. c-Myc is a vital oncogene frequently overexpressed or amplified in various cancer types, including breast cancer. Our previous study indicated its potential association with breast cancer stem cell (BCSC) biomarkers.  \nMethods In this research, we performed immunohistochemical (IHC) staining on sixty breast cancer surgical specimens for c-Myc, CD44, CD24, CD133 and ALDH1A1 . Then, we analyzed transcriptomic atlas of 1533 patients with breast cancer from public database.  \nResults IHC staining indicated the positive correlation between c-Myc and BCSC phenotype. Then, we used bioinformatic analysis to interrogate transcriptomics data of 1533 breast cancer specimens and identified an intriguing link among c-Myc, cancer stemness and copper-induced cell death (also known as “cuproptosis”) . We screened out cuproptosis-related characteristics that predicts poor clinical outcomes and found that the pro-tumoral cuproptosisbased features were putatively enriched in MYC-targets and showed a significantly positive correlation with cancer stemness.  \nConclusion In addition to previous reports on its oncogenic roles, c-Myc showed significant correlation to stemness phenotype and copper-induced cell toxicity in breast cancer tissues. Moreover, transcriptomics data demonstrated that pro-tumoral cuproptosis biomarkers had putative positive association with cancer stemness. This research combined clinical samples with large-scale bioinformatic analysis, covered description and deduction, bridged classic oncogenic mechanisms to innovative opportunities, and inspired the development of copper-based nanomaterials in targeting highly heterogeneous tumors.  \nKeywords Intra-tumoral heterogeneity, c-Myc, Cancer stem cell, Copper-induced cell death, Cuproptosis, Tumor microenvironment  \n†Runtian Wang, Kun Xu, Qin Chen and Qin Hu contributed equally to this work.  \n*Correspondence: Jian Zhang [syner2000@163.com](syner2000@163.com)[ ](syner2000@163.com)Xiaoxiang Guan [xguan@njmu.edu.cn](xguan@njmu.edu.cn)  \nFull list of author information is available at the end of the article  \n© The Author(s) 2023. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit [http://creativecommons.org/licenses/by/4.0/](http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver ()[. The Creative Commons Public Domain Dedication waiver (](http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver ()[http://creativeco](http://creativeco)[mmons.org/publicdomain/zero/1.0/](mmons.org/publicdomain/zero/1.0/)) applies to the","cbCaih9VOYM0ylrl","https://ap.wps.com/l/cbCaih9VOYM0ylrl","pdf",3888008,13,"English","# Abstract\n## Background\n## Methods\n## Results\n## Conclusion\n# Introduction","[{\"question\":\"What is the main relationship investigated between c-Myc and breast cancer stemness?\",\"answer\":\"The study finds a positive correlation between c-Myc and the breast cancer stem cell (BCSC) phenotype. Transcriptomic analysis links c-Myc, cancer stemness, and cuproptosis-related mechanisms.\"},{\"question\":\"How were the experimental and analytical datasets used in the study?\",\"answer\":\"Immunohistochemical staining was performed on 60 breast cancer surgical specimens to assess markers related to stemness and c-Myc. Separately, transcriptomic data from 1533 breast cancer patients were analyzed from public databases.\"},{\"question\":\"What does the study report about cuproptosis-related features and clinical outcomes?\",\"answer\":\"Cuproptosis-related characteristics were screened that predict poor clinical outcomes. The pro-tumoral cuproptosis-based features were enriched in MYC-targets and correlated positively with cancer stemness.\"}]","Cuproptosis engages in c-Myc-mediated breast cancer stemness - Research Open Access | PDF",1790258936,33]