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CSN5 is implicated in cancer biology, but its role in cervical cancer is not established. This study evaluates CSN5 and its target gene through bioinformatics, proteomic profiling, and in vitro/in vivo assays, showing that CSN5 upregulation correlates with advanced stage, poor differentiation, increased proliferation, and glycolysis. Surprisingly, CSN5 suppresses migration, invasion, and EMT. ENO3 is identified as a key mediator: CSN5 regulates ENO3 via ubiquitination-degradation, and ENO3 silencing reverses CSN5-driven oncogenic effects, supporting the CSN5-ENO3 axis as a therapeutic target.",{"@graph":69,"@context":121},[70,84,104],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/csn5-overexpression-promotes-the-integral-progression-of-cervical-cancer-by-enhancing-eno3-mediated-glycolysis/356249/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":98,"encodingFormat":97,"isAccessibleForFree":99,"interactionStatistic":100},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/csn5-overexpression-promotes-the-integral-progression-of-cervical-cancer-by-enhancing-eno3-mediated-glycolysis/356249.png","ImageObject",300,407,{"name":92,"@type":93},"Himbo","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-09-23",true,{"@type":101,"interactionType":102,"userInteractionCount":8},"InteractionCounter",{"@type":103},"ViewAction",{"@type":105,"mainEntity":106},"FAQPage",[107,113,117],{"name":108,"@type":109,"acceptedAnswer":110},"What is the main purpose of the study on CSN5 in cervical cancer?","Question",{"text":111,"@type":112},"To investigate CSN5’s role and its target gene in cervical cancer progression and to assess their potential as therapeutic targets.","Answer",{"name":114,"@type":109,"acceptedAnswer":115},"How does CSN5 overexpression affect cervical cancer cells and metabolism?",{"text":116,"@type":112},"CSN5 overexpression enhances cellular proliferation and glycolytic metabolism, while suppressing migration, invasion, and the epithelial-mesenchymal transition (EMT).",{"name":118,"@type":109,"acceptedAnswer":119},"What role does ENO3 play in CSN5-mediated tumorigenesis?",{"text":120,"@type":112},"ENO3 is regulated by CSN5 via ubiquitination-dependent stabilization and degradation control; silencing ENO3 attenuates the oncogenic effects of CSN5 both in vitro and in vivo.","https://schema.org",{"og:url":83,"og:type":123,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":125,"canonical":83},"index,follow",{"doc_id":127,"site_id":62},356249,1790198126,{"code":4,"msg":5,"data":130},{"doc_id":127,"user_id":131,"nickname":92,"user_avatar":132,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":133,"file_id":134,"file_url":135,"file_type":136,"file_size":137,"view_count":8,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":138,"language":139,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":140,"faqs":141,"seo_title":142,"seo_description":67,"update_tm":143,"read_time":144},687197100911,"https://ap-avatar.wpscdn.com/avatar/a000239b6f1da00475?x-image-process=image/resize,m_fixed,w_180,h_180&k=1785132997149421697","CSN5 overexpression promotes the integral progression of cervical cancer by enhancing ENO3-mediated glycolysis  \nZhu Cao1,2 · Shizhou Yang1,2 · Jian Zou1,2 · Ye Jin1,2 · Yanan Zhang3 · Tingting Chen1,2 · Yuanming Shen1,2  \nReceived: 25 April 2025 / Accepted: 17 December 2025 / Published online: 12 January 2026 © The Author(s) 2025  \nAbstract  \nCervical cancer (CC) remains a significant global health challenge. A deeper understanding of the molecular mechanisms driving CC progression is crucial for developing improved therapeutic strategies. CSN5 is vital in cell functions and cancer, but its role in CC is unclear. This study aims to explore CSN5’s role and its target gene in CC progression, assessing their potential as therapeutic targets. We employed an integrated approach combining bioinformatics analysis, proteomic profiling, and in vitro and in vivo functional assays. Immunohistochemistry was used to analyze CSN5 and ENO3 expression in CC and normal tissues. CSN5 upregulation was associated with advanced clinical stage and poor differentiation. Furthermore, CSN5 overexpression enhanced cellular proliferation and glycolytic metabolism but, paradoxically, suppressed migration, invasion, and the epithelial-mesenchymal transition (EMT). These pro-tumorigenic effects were confirmed in vivo, and the glycolytic inhibitor 2-DG was found to reverse the phenotypes induced by CSN5 . Protein sequencing highlighted ENO3’s role in CSN5-mediated tumorigenesis, regulating EMT and glycolysis by stabilizing its ubiquitination degradation through CSN5 . Crucially, silencing ENO3 attenuated the oncogenic effects of CSN5 both in vitro and in vivo. Our findings unveil a novel mechanistic paradigm in which CSN5 promotes CC progression by coopting ENO3 to enhance glycolytic flux while concurrently suppressing cell motility. This study not only deepens the understanding of CC pathogenesis but also identifies the CSN5-ENO3 axis as a promising target for novel therapeutic interventions.  \nZhu Cao, Shizhou Yang, Jian Zou have contributed equally to this work.  \n􀀍 Tingting Chen [11318160@zju.edu.cn](11318160@zju.edu.cn)  \n􀀍 Yuanming Shen [5312010@zju.edu.cn](5312010@zju.edu.cn)  \n1 Department of Gynecology and Oncology, Women’s Hospital, School of Medicine, Zhejiang University, 1\\# Xueshi Road, Shangcheng District, Hangzhou 310006, Zhejiang Province, People’s Republic of China  \n2 Zhejiang Provincial Key Laboratory of Precision Diagnosis and Therapy for Major Gynecological Diseases, Women’s Hospital, School of Medicine, Zhejiang University, Hangzhou 310006, Zhejiang, People’s Republic of China  \n3 Department of Obstetrics and Gynecology, Peking University People’s Hospital, Beijing  \n100032, People’s Republic of China  \nGraphical abstract  \nKey messages  \n1. CSN5 overexpression promotes cervical cancer progression via glycolysis.  \n2. CSN5 enhances ENO3 stability, inhibiting its ubiquitination and degradation.  \n3. CSN5 suppresses EMT while boosting tumor cell proliferation and glycolysis.  \n4. ENO3 knockdown reverses CSN5-driven tumor growth and metabolic changes.  \n5. CSN5-ENO3 axis identified as a potential therapeutic target in cervical cancer.  \nKeywords Cervical cancer · CSN5 · Glycolysis · Cell motility · ENO3  \nIntroduction  \nCervical cancer (CC) is the third most common cancer among US women and the most prevalent among women in developing countries, accounting for 15% of global female cancers [1] . In the 1980s, German virologist Harold Zur Hausen established a link between CC and Human Papillomavirus (HPV) infections, paving the way for HPV vaccination and screening programs [2]. Additionally, CC treatment varies by stage, with surgery for early stages and a combination of brachytherapy and chemoradiation for advanced stages [3] . Despite being preventable, it remains a major cause of premature death and cancer mortality, especially in low- and medium-income countries, highlighting the need for better prevention and advanced treatment [4]. ","cbCaikHMrEbC0kFe","https://ap.wps.com/l/cbCaikHMrEbC0kFe","pdf",2989187,17,"English","# Abstract\n# Graphical abstract\n# Key messages\n# Keywords\n# Introduction","[{\"question\":\"What is the main purpose of the study on CSN5 in cervical cancer?\",\"answer\":\"To investigate CSN5’s role and its target gene in cervical cancer progression and to assess their potential as therapeutic targets.\"},{\"question\":\"How does CSN5 overexpression affect cervical cancer cells and metabolism?\",\"answer\":\"CSN5 overexpression enhances cellular proliferation and glycolytic metabolism, while suppressing migration, invasion, and the epithelial-mesenchymal transition (EMT).\"},{\"question\":\"What role does ENO3 play in CSN5-mediated tumorigenesis?\",\"answer\":\"ENO3 is regulated by CSN5 via ubiquitination-dependent stabilization and degradation control; silencing ENO3 attenuates the oncogenic effects of CSN5 both in vitro and in vivo.\"}]","CSN5 overexpression promotes the integral progression of cervical cancer by enhancing ENO3-mediated glycolysis | PDF",1790124252,43]