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Because genomic alterations drive tumor initiation and progression, CRISPR-Cas9 has been used to identify and modulate key cancer genes. A literature review summarizes the technology’s fundamentals and highlights clinical and preclinical studies across solid tumors and hematologic malignancies, emphasizing advances in immune-mediated cell therapies.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/crispr-cas9-in-basic-and-translational-aspects-of-cancer-therapy-review/332690/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/crispr-cas9-in-basic-and-translational-aspects-of-cancer-therapy-review/332690.png","ImageObject",300,407,{"name":92,"@type":93},"Skyler","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-26","2026-09-21",true,{"@type":102,"interactionType":103,"userInteractionCount":39},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What is the main purpose of using CRISPR-Cas9 in cancer research?","Question",{"text":112,"@type":113},"To precisely target DNA and edit specific sequences so that genes involved in tumor initiation and progression can be identified and manipulated.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How was the review conducted?",{"text":117,"@type":113},"It was written by reviewing existing literature and searching databases such as PubMed and Google Scholar, along with ClinicalTrials.gov for clinical trial information.",{"name":119,"@type":110,"acceptedAnswer":120},"Which cancer types and therapeutic areas are discussed in the review’s results?",{"text":121,"@type":113},"The review addresses solid tumors and hematologic neoplasms and describes progress in boosting immune-mediated cell therapy in oncology, including CAR-T, CAR-NK, and CAR-M cell approaches.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},332690,1790019404,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":39,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":145,"preview_page_count":4},2336464648746,"https://ap-avatar.wpscdn.com/davatar_276721f389ce27ea32af1340a28f341c","CRISPR-Cas9 in basic and translational aspects of cancer therapy  \nMaryam Samareh Salavatipour1 ID , Zahra Poursalehi2, Negin Hosseini Rouzbahani3, Sohaib Mohammadyar4, Mohammad Vasei5* ID  \n1Department of Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran  \n2Department of Applied Cell Sciences, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran 3Department of Medical Immunology, Faculty of Medicine, Aja University of Medical Sciences, Tehran, Iran  \n4Department of Hematology and Laboratory Sciences, Faculty of Allied Medicine, Kerman University of Medical Sciences, Kerman, Iran 5Gene Therapy Research Center, Digestive Disease Research Institute, Tehran University of Medical Sciences, Tehran, Iran  \nArticle Info  \nArticle Type:  \nReview  \nArticle History:  \nReceived: 2 Sep. 2023  \nRevised: 4 Jan. 2024  \nAccepted: 9 Jan. 2024  \nePublished: 10 Mar. 2024  \nKeywords:  \nCRISPR-Cas9  \nGene editing Cancer  \nSolid tumors Hematologic malignancy Immunotherapy Diagnosis  \nDrug resistance  \nAbstract  \nIntroduction: The discovery of gene editing techniques has opened a new era within the field of biology and enabled scientists to manipulate nucleic acid molecules. CRISPRCas9 genome engineering has revolutionized this achievement by successful targeting the DNA molecule and editing its sequence. Since genomic changes are the basis of the birth and growth of many tumors, CRISPRCas9 method has been successfully applied to identify and manipulate the genes which are involved in initiating and driving some neoplastic processes.  \nMethods: By review of the existing literature  \non application of CRISPR-Cas9 in cancer, different databases, such as PubMed and Google Scholar, we started data collection for \"CRISPR-Cas9\", \"Genome Editing\", \"Cancer\", \"Solid tumors\", \"Hematologic malignancy\" \"Immunotherapy\", \"Diagnosis\", \"Drug resistance\" phrases. [Clinicaltrials.gov](Clinicaltrials.gov), a resource that provides access to information on clinical trials, was also searched in this review.  \nResults: We have defined the basics of this technology and then mentioned some clinical and preclinical studies using this technology in the treatment of a variety of solid tumors as well ashematologic neoplasms. Finally, we described the progress made by this technology in boosting immune-mediated cell therapy in oncology, such as CAR-T cells, CAR-NK cells, and CAR-M cells. Conclusion: CRISPR-Cas9 system revolutionized the therapeutic strategies in some solid malignant tumors and leukemia through targeting the key genes involved in the pathogenesis of these cancers.  \nIntroduction  \nDNA repair by deletion or replacement of the sequences involved in diseases and generation of nonpathogenic genes are the main areas of genetic manipulation in gene therapy. These therapeutic approaches are promising strategies for treating many non-curable human diseases, such as some hemoglobinopathies, acquired immunodeficiency syndrome (AIDS), diabetes, metabolic and neurodegenerative diseases, and cancer.1-3 The precise manipulation of the target regions, including specific gene activation or inactivation, nucleotide sequence  \nreplacement, gene deletion, and rearrangement, usually enables appropriate nucleases to be embedded in the editing platforms.3 At the first attempts, few products purported to be impressive in the treatment of patients with head and neck carcinomas and certain brain tumors. These products used a cut-and-paste technique to eliminate defects and insert the correct gene in the right place on the chromosome.3 However, these initial gene therapy tools integrated a copy of exogenous DNA into the genome with undesirable effects, including abnormal protein expression and insertion mutations. In recent  \n*Corresponding author: Mohammad Vasei, [Email: mvasei@tums.ac.ir](Email: mvasei@tums.ac.ir)  \n © 2024 The Author(s) . This work is published by BioImpacts as an open access artic","cbCair1XAyEZLEwZ","https://ap.wps.com/l/cbCair1XAyEZLEwZ","pdf",2130518,21,"English","# Abstract\n## Introduction\n## Methods\n## Results\n## Conclusion\n# Different kinds of genome editing tools","[{\"question\":\"What is the main purpose of using CRISPR-Cas9 in cancer research?\",\"answer\":\"To precisely target DNA and edit specific sequences so that genes involved in tumor initiation and progression can be identified and manipulated.\"},{\"question\":\"How was the review conducted?\",\"answer\":\"It was written by reviewing existing literature and searching databases such as PubMed and Google Scholar, along with ClinicalTrials.gov for clinical trial information.\"},{\"question\":\"Which cancer types and therapeutic areas are discussed in the review’s results?\",\"answer\":\"The review addresses solid tumors and hematologic neoplasms and describes progress in boosting immune-mediated cell therapy in oncology, including CAR-T, CAR-NK, and CAR-M cell approaches.\"}]","CRISPR-Cas9 in basic and translational aspects of cancer therapy - Review | PDF",1789999093,53]