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Using immunohistochemistry, CKMT2 expression was profiled in CRC and adjacent tissues, then correlated with clinicopathological features and patient prognosis via Kaplan-Meier and Cox analyses. CKMT2 was quantified in CRC cell lines by qRT-PCR, and functional effects and mechanisms were examined with multiple molecular and cell assays, revealing CKMT2 as an independent prognostic factor that promotes aerobic glycolysis through interaction with LDHB.",{"@graph":69,"@context":126},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/creatine-kinase-mitochondrial-2-promotes-the-growth-and-progression-of-colorectal-cancer-via-enhancing-warburg-effect-through-lactate-dehydrogenase-b/333513/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/creatine-kinase-mitochondrial-2-promotes-the-growth-and-progression-of-colorectal-cancer-via-enhancing-warburg-effect-through-lactate-dehydrogenase-b/333513.png","ImageObject",300,407,{"name":92,"@type":93},"Violet","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-28","2026-09-21",true,{"@type":102,"interactionType":103,"userInteractionCount":34},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118,122],{"name":109,"@type":110,"acceptedAnswer":111},"What is the main research focus regarding CKMT2 in colorectal cancer?","Question",{"text":112,"@type":113},"The study investigates CKMT2 expression in CRC, its clinical significance, and how CKMT2 influences CRC biology at the molecular level.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How were CKMT2 expression levels and patient prognosis analyzed?",{"text":117,"@type":113},"CKMT2 expression was assessed by immunohistochemical staining, correlated with clinicopathological factors, and evaluated for prognostic value using Kaplan-Meier survival curves and Cox regression.",{"name":119,"@type":110,"acceptedAnswer":120},"What functional role does CKMT2 play in CRC cell behavior?",{"text":121,"@type":113},"Experimental downregulation of CKMT2 inhibited migration and promoted apoptosis in CRC cell lines.",{"name":123,"@type":110,"acceptedAnswer":124},"How does CKMT2 relate to aerobic glycolysis and the Warburg effect?",{"text":125,"@type":113},"The study identifies CKMT2 as promoting aerobic glycolysis in CRC cells by interacting with lactate dehydrogenase B (LDHB), thereby enhancing the Warburg effect.","https://schema.org",{"og:url":83,"og:type":128,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":130,"canonical":83},"index,follow",{"doc_id":132,"site_id":62},333513,1790180541,{"code":4,"msg":5,"data":135},{"doc_id":132,"user_id":136,"nickname":92,"user_avatar":137,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":138,"file_id":139,"file_url":140,"file_type":141,"file_size":142,"view_count":34,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":143,"language":144,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":145,"faqs":146,"seo_title":147,"seo_description":67,"update_tm":148,"read_time":149,"preview_page_count":4},4398048950312,"https://ap-avatar.wpscdn.com/avatar/400002538284de19e3c?_k=1778320343897328908","Submitted 15 January 2024 Accepted 12 June 2024  \nPublished 28 June 2024  \nCorresponding authors Zaixing Yang, [yangzaixingdiyi@163.com](yangzaixingdiyi@163.com)[ ](yangzaixingdiyi@163.com)Changfa Yu, [yuchangfa123@163.com](yuchangfa123@163.com)  \nAcademic editor Hilal Ozdag Sevgili  \nAdditional Information and Declarations can be found on page 19  \nDOI 10.7717/peerj.17672  \nCopyright 2024 Cai et al.  \nDistributed under  \nCreative Commons CC-BY 4.0  \nCreatine kinase mitochondrial 2 promotes the growth and progression of colorectal cancer via enhancing Warburg effect through lactate dehydrogenase B  \nShasha Cai 1, *, Qingqing Xia 1, *, Darong Duan 1, Junhui Fu2, Zhenxing Wu3, Zaixing Yang1 and Changfa Yu 1  \n1 Laboratory Medicine, Taizhou First People’s Hospital, Huangyan Hospital of Wenzhou Medical University, Taizhou, China  \n2 General Surgery, Taizhou First People’s Hospital, Huangyan Hospital of Wenzhou Medical University, Taizhou, China  \n3 Gastroenterology, Taizhou First People’s Hospital, Huangyan Hospital of Wenzhou Medical University, Taizhou, China  \n* These authors contributed equally to this work.  \nABSTRACT  \nBackground: Mitochondrial creatine kinase (MtCK) plays a pivotal role in cellular energy metabolism, exhibiting enhanced expression in various tumors, including colorectal cancer (CRC) . Creatine kinase mitochondrial 2 (CKMT2) is a subtype of MtCK; however, its clinical significance, biological functions, and underlying molecular mechanisms in CRC remain elusive.  \nMethods: We employed immunohistochemical staining to discern the expression of CKMT2 in CRC and adjacent nontumor tissues of patients.  \nThe correlation between CKMT2 levels and clinical pathological factors was assessed. Additionally, we evaluated the association between CKMT2 and the prognosis of CRC patients using Kaplan-Meier survival curves and Cox regression analysis.  \nMeanwhile, quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to detect the expression levels ofCKMT2 in different CRC cell lines. Finally, we explored the biological functions and potential molecular mechanisms ofCKMT2  \nin CRC cells through various techniques, including qRT-PCR, cell culture, cell transfection, western blot, Transwell chamber assays, flow cytometry, and co-immunoprecipitation.  \nResults: We found that CKMT2 was significantly overexpressed in CRC tissues compared with adjacent nontumor tissues. The expression of CKMT2 is correlated with pathological types, tumor size, distant metastasis, and survival in CRC patients. Importantly, CKMT2 emerged as an independent prognostic factor through Cox regression analysis. Experimental downregulation of CKMT2 expression in CRC cell lines inhibited the migration and promoted apoptosis of these cells. Furthermore, weidentified a novel role for CKMT2 in promoting aerobic glycolysis in CRC cells through interaction with lactate dehydrogenase B (LDHB) .  \nConclusion: In this study, we found the elevated expression ofCKMT2 in CRC, and it was a robust prognostic indicator in CRC patients. CKMT2 regulates glucose metabolism via amplifying the Warburg effect through interaction with LDHB, which promotes the growth and progression of CRC. These insights unveil a novel  \nHow to cite this article Cai S, Xia Q, Duan D, Fu J, Wu Z, Yang Z, Yu C. 2024. Creatine kinase mitochondrial 2 promotes the growth and progression of colorectal cancer via enhancing Warburg effect through lactate dehydrogenase B. PeerJ 12:e17672 DOI 10.7717/peerj.17672  \nregulatory mechanism by which CKMT2 influences CRC and provide promising targets for future CRC therapeutic interventions.  \nSubjects Cell Biology, Molecular Biology, Gastroenterology and Hepatology, Oncology, Histology Keywords Creatine kinase mitochondrial 2, Colorectal cancer, Warburg effect, Lactate dehydrogenase B  \nINTRODUCTION  \nColorectal cancer (CRC) stands as the most prevalent malignant tumor in the digestive system worldwide, ranking third in incidence and second ","cbCaigVc1PmjMbat","https://ap.wps.com/l/cbCaigVc1PmjMbat","pdf",31640983,22,"English","# Abstract\n## Methods\n## Results\n## Conclusion\n# Introduction","[{\"question\":\"What is the main research focus regarding CKMT2 in colorectal cancer?\",\"answer\":\"The study investigates CKMT2 expression in CRC, its clinical significance, and how CKMT2 influences CRC biology at the molecular level.\"},{\"question\":\"How were CKMT2 expression levels and patient prognosis analyzed?\",\"answer\":\"CKMT2 expression was assessed by immunohistochemical staining, correlated with clinicopathological factors, and evaluated for prognostic value using Kaplan-Meier survival curves and Cox regression.\"},{\"question\":\"What functional role does CKMT2 play in CRC cell behavior?\",\"answer\":\"Experimental downregulation of CKMT2 inhibited migration and promoted apoptosis in CRC cell lines.\"},{\"question\":\"How does CKMT2 relate to aerobic glycolysis and the Warburg effect?\",\"answer\":\"The study identifies CKMT2 as promoting aerobic glycolysis in CRC cells by interacting with lactate dehydrogenase B (LDHB), thereby enhancing the Warburg effect.\"}]","Creatine kinase mitochondrial 2 promotes the growth and progression of colorectal cancer via enhancing Warburg effect through lactate dehydrogenase B | PDF",1790002829,55]