[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-159326-105":59,"doc-detail-159326-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","cpn-680-conjugated-polymer-nanoparticles-product-sheet","CPN™ 680 Conjugated Polymer Nanoparticles - Product Sheet","","CPN™ 680 conjugated polymer nanoparticles are highly fluorescent, water-soluble micelles with semiconductor polymer cores encapsulated in a biocompatible surfactant. Their brightness and exceptional photo-, thermo-, and chemical stability enable sensitive detection across multiple molecular biology workflows. Surface-accessible carboxylic groups support covalent conjugation of antibodies, streptavidin, proteins, and nucleic acids via EDC chemistry, while incorporated iron oxide enables magnetic manipulation and MRI contrast. Applications include flow cytometry, imaging, ELISA, ISH, and Western blotting, with stable emission across the visible range.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":30,"@type":76,"position":81},"https://docshare.wps.com/document/technology/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/cpn-680-conjugated-polymer-nanoparticles-product-sheet/159326/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/cpn-680-conjugated-polymer-nanoparticles-product-sheet/159326.png","ImageObject",300,407,{"name":92,"@type":93},"Aria Callaghan","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-09-27","2026-08-29",true,{"@type":102,"interactionType":103,"userInteractionCount":29},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What makes CPN™ 680 nanoparticles highly fluorescent for in vitro labeling?","Question",{"text":112,"@type":113},"CPN™ 680 combines semiconductor light-emitting polymer cores with intense fluorescence driven by exceptional extinction coefficients and strong optical stability, supporting detection with very high sensitivity.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How are antibodies or other targeting molecules conjugated to CPN™ 680?",{"text":117,"@type":113},"Conjugation is performed using EDC chemistry to link protein amine groups (-NH2) to CPN™ surface carboxyl groups (-COOH). A titration of CPN™:targeting molecule ratios is recommended to find optimal conditions.",{"name":119,"@type":110,"acceptedAnswer":120},"What role does iron oxide play in CPN™ 680 applications?",{"text":121,"@type":113},"Iron oxide allows magnetic manipulation for purification and quantification, and it enables visualization as an MRI contrast agent.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},159326,1788016388,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":29,"category_name":30,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":29,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":81,"language":139,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":140,"faqs":141,"seo_title":142,"seo_description":67,"update_tm":129,"read_time":39},962084926284,"https://ap-avatar.wpscdn.com/davatar_29158cc5080c5b710cf443261637dec0","Conjugated Polymer  \nNanoparticles (CPNs™) CPN™ 680  \nCPNs™ are highly fluorescent nanoparticles containing semiconductor light emitting polymer cores encapsulated within a surfactant. CPNs™ have fluorescent properties significantly exceeding those of other in vitro labelling agents, intense brightness due to exceptional extinction coefficients and outstanding photo- , thermo- and chemical stability. CPNs ™ have shown no toxicity and are ideal for use with live cell systems. CPNs™ are enabled with surface accessible carboxylic groups for the conjugation of a wide range of molecules including antibodies, streptavidin and nucleic acids. This allows CPNs™ to be used in a diverse array of binding and targeting applications, such as flow cytometry, immunohistochemistry, immunocytochemistry, and high content screening. CPNs™ also incorporate iron oxide, allowing their magnetic manipulation and the purification and quantification of bound molecules or cells. Further, the iron oxide allows CPNs™ tobe used as contrast agents in Magnetic Resonance Imaging (MRI) . CPNs™ range in fluorescence emission wavelengths from 450 nm to 680 nm.  \nConjugated targeting moieties bound to surface:  \ne.g. Antibody, oligonucleotide, protein, streptavidin  \nIron Oxide  \nCapping molecules Increases hydrophilicity  \nFluorescent  \nHydrophobic polymer core  \nApplications  \n\n| • Flow cytometry – Cell surface, low abundance targets |\n| --- |\n| • Cell imaging/tracking – CPNs loaded in to by endocytosis |\n| • Immunohistochemistry – Readily linked to secondary Ab |\n| • Fluorescent ELISA – Signal is time independent |\n| • Fluorescent In Situ Hybridisation – Sensitive and stable signal |\n| • Western blotting-Allows linear quantification of signal |\n\nHCC70 cells passively loaded with CPN510  \nover 3 days in culture  \nStructural Properties:  \nConjugated Polymer Nanoparticles are water-soluble micelles compromising of a Light Emitting Polymer and are around 100 nm in size. CPNs™ exhibit luminescence or fluorescence with light emission wavelengths from 475 nm to 680 nm. They are encapsulated within a biocompatible surfactant, increasing the hydrophilicity and allowing them to form micelles. This ‘core-shell’ structure, consisting of the polymer forming the core and the surfactant the surrounding shell, provides a ready base on which to covalently bond functionalising molecules, such streptavidin, antibodies, targeting proteins or nucleic acids. CPNs™ also incorporate iron oxide into their core. This allows CPNs™, and the molecules or cells to which they are attached, to be manipulated using magnets to direct movement and facilitate purification. The iron oxide can be also be visualised using Magnetic Resonance Imaging (MRI), acting as a contrast reagent.  \nBiological Properties:  \nCPNs™ are readily conjugated to biomolecules such as antibodies or streptavidin allowing their use in a wide range of molecular biological applications. The intense brightness of the CPNs™ dramatically increases the sensitivity of these applications, with single nanoparticles being detectable in flow cytometry and immunocytochemistry, allowing the study of individual proteins in samples and cells. The streptavidin and antibodies are covalently conjugated to the CPNs™ via the surfactant’s carboxylic acid groups using Nethyl-N’-dimethylaminopropyl-carbodiimide (EDC) chemistry. The CPNs™ conjugates can be used in ‘end user’ assays at concentrations matching those of other conjugated fluorophores. Due to differences in assay systems working dilutions should be determined by titration assay. The CPNs™ are both thermal and photostable, however once conjugated to biological materials, they should be stored at 2-6°C.  \nOptical Properties:  \nThe color of light emitted by CPNs™ is dependent on the specific polymer core creating a common platform of labels across the visible spectrum. CPNs™ are extremely bright and photostable, with samples stored under ambient temperature and lighting conditions retain","cbCaiqva40seTvuO","https://ap.wps.com/l/cbCaiqva40seTvuO","pdf",362142,"English","# Key Features\n## Fluorescence and Optical Range\n## Magnetic Functionality\n# Structural and Biological Properties\n## Core-shell Micelle Design\n## Conjugation Chemistry and Stability\n# Applications\n## Flow Cytometry and Cell Imaging\n## Immunoassays and Molecular Detection\n# Protocol: EDC Conjugation to Targeting Molecules\n## Preparation and Reaction Steps\n## Purification and Resuspension\n# Materials and Storage\n## Example Batch Specifications\n# Technical Specifications\n## Hydrodynamic Diameter and Excitation/Emission","[{\"question\":\"What makes CPN™ 680 nanoparticles highly fluorescent for in vitro labeling?\",\"answer\":\"CPN™ 680 combines semiconductor light-emitting polymer cores with intense fluorescence driven by exceptional extinction coefficients and strong optical stability, supporting detection with very high sensitivity.\"},{\"question\":\"How are antibodies or other targeting molecules conjugated to CPN™ 680?\",\"answer\":\"Conjugation is performed using EDC chemistry to link protein amine groups (-NH2) to CPN™ surface carboxyl groups (-COOH). A titration of CPN™:targeting molecule ratios is recommended to find optimal conditions.\"},{\"question\":\"What role does iron oxide play in CPN™ 680 applications?\",\"answer\":\"Iron oxide allows magnetic manipulation for purification and quantification, and it enables visualization as an MRI contrast agent.\"}]","CPN™ 680 Conjugated Polymer Nanoparticles - Product Sheet | PDF"]