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Gaining deep insight into how cancer initiates and progresses requires methods that study cancer cells at the single-cell level, capturing key parameters such as cell morphology, metabolism, and molecular characteristics. Recent microscopy advances highlight Raman microspectroscopy as a powerful label-free approach that provides biochemical and spatial information and can be correlated with other modalities to support complementary understanding for early diagnosis and monitoring.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/correlative-raman-imaging-development-and-cancer-applications-review/331013/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/correlative-raman-imaging-development-and-cancer-applications-review/331013.png","ImageObject",300,407,{"name":92,"@type":93},"Noah","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-11-04","2026-09-21",true,{"@type":102,"interactionType":103,"userInteractionCount":29},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"Why is single-cell methodology important for studying cancer?","Question",{"text":112,"@type":113},"It enables examination of critical parameters including cell morphology, metabolism, and molecular characteristics, supporting clearer differentiation between healthy and cancerous cells and better understanding of tumor progression.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"What makes Raman microspectroscopy (RM) effective for cancer cell analysis?",{"text":117,"@type":113},"RM provides biochemical and spatial details at the single-cell level without labels and without disrupting cell integrity, and it can be correlated with other microscopy techniques.",{"name":119,"@type":110,"acceptedAnswer":120},"Which microscopy techniques are discussed as correlation partners for RM?",{"text":121,"@type":113},"The review focuses on correlations between RM and confocal fluoresce microscopy, atomic force microscopy, digital holography microscopy, and mass spectrometry imaging, each contributing distinct strengths.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},331013,1790313746,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":29,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":36,"preview_page_count":4},8796095462418,"https://ap-avatar.wpscdn.com/avatar/80000253c1241d02b47?x-image-process=image/resize,m_fixed,w_180,h_180&k=1778826106357471780","biosensors  \nReview  \nCorrelative Raman Imaging: Development and Cancer Applications  \nHossein Khadem †, Maria Mangini †, Somayeh Farazpour and Anna Chiara De Luca *  \nCitation: Khadem, H.; Mangini, M.; Farazpour, S.; De Luca, A.C.  \nCorrelative Raman Imaging: Development and Cancer Applications. Biosensors 2024, 14, 324 . [https://doi.org/10.3390/bios14070324](https://doi.org/10.3390/bios14070324)  \nReceived: 24 May 2024  \nRevised: 26 June 2024  \nAccepted: 27 June 2024  \nPublished: 28 June 2024  \nCopyright: © 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license ([https://](https://)[ ](https://)[creativecommons.org/licenses/by/](creativecommons.org/licenses/by/)[ ](creativecommons.org/licenses/by/)[4.0/](4.0/)) .  \nInstitute for Experimental Endocrinology and Oncology ‘G. Salvatore’, IEOS-Second Unit, National Research  \nCouncil, 80131 Naples, Italy; [h.khadem@ieos.cnr.it](h.khadem@ieos.cnr.it) (H.K.); [m.mangini@ieos.cnr.it](m.mangini@ieos.cnr.it) (M.M.);  \n[s.farazpour@ieos.cnr.it](s.farazpour@ieos.cnr.it) (S.F.)  \n* Correspondence: [annachiara.deluca@cnr.it](annachiara.deluca@cnr.it)[ ](annachiara.deluca@cnr.it)† These authors contributed equally to this work.  \nAbstract: Despite extensive research efforts, cancer continues to stand as one of the leading causes of death on a global scale. To gain profound insights into the intricate mechanisms underlying cancer onset and progression, it is imperative to possess methodologies that allow the study of cancer cells at the single-cell level, focusing on critical parameters such as cell morphology, metabolism, and molecular characteristics. These insights are essential for effectively discerning between healthy and cancerous cells and comprehending tumoral progression. Recent advancements in microscopy techniques have significantly advanced the study of cancer cells, with Raman microspectroscopy (RM) emerging as a particularly powerful tool. Indeed, RM can provide both biochemical and spatial details at the single-cell level without the need for labels or causing disruptions to cell integrity. Moreover, RM can be correlated with other microscopy techniques, creating a synergy that offers a spectrum of complementary insights into cancer cell morphology and biology. This review aims to explore the correlation between RM and other microscopy techniques such as confocal fluoresce microscopy (CFM), atomic force microscopy (AFM), digital holography microscopy (DHM), and mass spectrometry imaging (MSI) . Each of these techniques has their own strengths, providing different perspectivesand parameters about cancer cell features. The correlation between information from these various analysis methods is a valuable tool for physicians and researchers, aiding in the comprehension of cancer cell morphology and biology, unraveling mechanisms underlying cancer progression, and facilitating the development of early diagnosis and/or monitoring cancer progression.  \nKeywords: cancer; Raman imaging; Raman spectroscopy; correlative imaging; fluorescence microscopy; atomic force microscopy; quantitative phase imaging; digital holography microscopy; mass spectroscopy imaging  \n1. Introduction  \nCancer, a multifaceted disease characterized by uncontrolled cell growth, poses a significant global health challenge. Early diagnosis plays a pivotal role in combating its overwhelming impact. Detecting cancer at its emerging stage offers a window of opportunity for successful treatment and improved patient outcomes. Early intervention not only enhances the effectiveness of therapeutic measures but also diminishes the chances of cancer spreading to other parts of the body, ultimately improving patient outcomes and quality of life.  \nDespite extensive research efforts, cancer remains one of the leading causes of death globally, exerting a significant burden on individuals and","cbCaiqRNDRxWIkCu","https://ap.wps.com/l/cbCaiqRNDRxWIkCu","pdf",2584947,16,"English","# Abstract\n# Keywords\n# 1. Introduction","[{\"question\":\"Why is single-cell methodology important for studying cancer?\",\"answer\":\"It enables examination of critical parameters including cell morphology, metabolism, and molecular characteristics, supporting clearer differentiation between healthy and cancerous cells and better understanding of tumor progression.\"},{\"question\":\"What makes Raman microspectroscopy (RM) effective for cancer cell analysis?\",\"answer\":\"RM provides biochemical and spatial details at the single-cell level without labels and without disrupting cell integrity, and it can be correlated with other microscopy techniques.\"},{\"question\":\"Which microscopy techniques are discussed as correlation partners for RM?\",\"answer\":\"The review focuses on correlations between RM and confocal fluoresce microscopy, atomic force microscopy, digital holography microscopy, and mass spectrometry imaging, each contributing distinct strengths.\"}]","Correlative Raman Imaging: Development and Cancer Applications - Review | PDF",1789991082]