[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-385498-105":59,"doc-detail-385498-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","comprehensive-profile-and-contrastive-analysis-of-circular-rna-expression-in-cervical-squamous-carcinoma-and-adenocarcinoma","Comprehensive profile and contrastive analysis of circular RNA expression in cervical squamous carcinoma and adenocarcinoma","","The study investigates and contrasts circular RNA (circRNA) expression patterns across cervical squamous carcinoma (SCC) and adenocarcinoma (ADC), including adenosquamous carcinoma (ASC), and compares them with adjacent normal tissues (ANT). High-throughput RNA sequencing profiles circRNAs, followed by bioinformatics analysis and qRT-PCR validation. Differentially expressed circRNAs are linked to enriched pathways and used to construct a circRNA–miRNA–mRNA network and a ceRNA network.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/comprehensive-profile-and-contrastive-analysis-of-circular-rna-expression-in-cervical-squamous-carcinoma-and-adenocarcinoma/385498/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/comprehensive-profile-and-contrastive-analysis-of-circular-rna-expression-in-cervical-squamous-carcinoma-and-adenocarcinoma/385498.png","ImageObject",300,407,{"name":92,"@type":93},"LangkahRina","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-27","2026-09-24",true,{"@type":102,"interactionType":103,"userInteractionCount":34},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What is the study’s primary goal?","Question",{"text":112,"@type":113},"To investigate and contrast circRNA expression patterns in cervical squamous carcinoma and adenocarcinoma, and to interpret their role in tumor differentiation mediated by HPV.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How were circRNA expression profiles obtained and validated?",{"text":117,"@type":113},"circRNA expression in SCC, ADC, ASC, and adjacent normal tissues was profiled by high-throughput RNA-seq, followed by bioinformatics analysis and qRT-PCR validation of sequencing results.",{"name":119,"@type":110,"acceptedAnswer":120},"Which enriched pathway and key circRNAs were emphasized?",{"text":121,"@type":113},"The ubiquitin mediated proteolysis pathway was the most enriched across the three cervical cancer variants, and eight key candidate circRNAs derived from this pathway were further validated and used to build a ceRNA network.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},385498,1790592692,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":34,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":145},962090893776,"https://ap-avatar.wpscdn.com/davatar_155a257f0dc6eb9ab79c44ca47cae57d","Submitted 22 September 2022 Accepted 27 December 2022 Published 26 January 2023  \nCorresponding authors Hongxue Luo, [louzhongkong@163.com](louzhongkong@163.com)[ ](louzhongkong@163.com)Yue Wang, [2633202441@qq.com](2633202441@qq.com)  \nAcademic editor Efe Sezgin  \nAdditional Information and Declarations can be found on page 16  \nDOI 10.7717/peerj.14759  \n Copyright 2023 Luo et al.  \nDistributed under  \nCreative Commons CC-BY 4.0  \nOPEN ACCESS  \nComprehensive profile and contrastive analysis of circular RNA expression in cervical squamous carcinoma andadenocarcinoma  \nHongxue Luo, Yi Zhu, Jiaqi Wang, Yue Wang and Lihui Wei  \nDepartment of Gynecology and Obstetrics, Peking University People'Hospital, Peking University, Beijing, China  \nABSTRACT  \nBackground. Numerous studies have shown circular RNA (circRNA) dysregulation is associated with the pathogenesis of cervical cancer,particularly in individual carcinoma variants. The aim of this study is to investigate and contrastively analyze the expression pattern of circRNAs in cervical squamous carcinoma and adenocarcinoma mediated by human papillomavirus type 16 (HPV-16) .  \nMethods. The expression ofcircRNAs in cervical squamous carcinoma (SCC), adenocarcinoma (ADC) and adenosquamous carcinoma (ASC) tissues, together with the adjacent normal tissues (ANT), was profiled by high-throughput RNA sequencing (RNA-seq) . Bioinformatics analysis and quantitative real time polymerase chain reaction (qRT-PCR) validation of the sequencing data were performed. A network ofcircRNA-miRNA (microRNA)-mRNA was then constructed according to predicted targets and function of candidate circRNAs.  \nResults. A total of 11,685 annotated circRNAs were identified in six cervical samples. There were 42 up-regulated and 98 down-regulated circRNAs. 215 circRNAs were upregulated in SCC but down-regulated circRNAs in ADC, while 50 circRNAs displayed the opposite trend. Function enrichment analysis based on different expressions ofcircRNAs found that the most enriched pathway in all the three pathologic variants of cervical cancer was the ``ubiquitin mediated proteolysis'' pathway. Eight key candidate circRNAs derived from this pathway were further validated, and we noticed that several target miRNAs of candidate circRNAs could target the source genes. Based on this we constructed a related competing endogenous RNA (ceRNA) network.  \nConclusion. Through a comprehensive interpretation of differentially expressed circRNAs in different pathologic variants of cervical cancer, this study provides new insights into the process of tumor differentiation mediated by HPV. Our results may help to complement the molecular typing and stem cell theory of cervical cancer.  \nSubjects Bioinformatics, Molecular Biology, Gynecology and Obstetrics, Women's Health Keywords Circular RNA, Expression profile, Cervical squamous carcinoma,  \nCervical adenocarcinoma, High-throughput RNA sequencing, Tumor differentiation, Bioinformatics analysis, Ubiquitin mediated proteolysis  \nHow to cite this article Luo H, Zhu Y, Wang J, Wang Y, Wei L. 2023. Comprehensive profile and contrastive analysis of circular RNA expression in cervical squamous carcinoma and adenocarcinoma. PeerJ 11:e14759 [http://doi.org/10.7717/peerj.14759](http://doi.org/10.7717/peerj.14759)  \nINTRODUCTION  \nCervical squamous cell carcinoma (SCC) and cervical adenocarcinoma (ADC) are two major histological variants of cervical malignant tumors (Cree et al., 2020) . Almost all SCC and over 85% of ADC are associated with human papilloma virus (HPV) infections, particularly HPV type 16 and 18 (De Sanjose et al., 2010; Pirog et al., 2000) . In the past decades, HPV vaccination programs and screening strategies of primary HPV have dramatically reduced SCCs worldwide (Bray et al., 2018; Schiffman et al., 2011) . Yet, ADC appears to be increasing in both absolute and relative incidence, especially among young women (Bray et al., 2005; Chen et al., 2016; Siegel, Miller & Jemal, 20","cbCaipLWvMy6tYXI","https://ap.wps.com/l/cbCaipLWvMy6tYXI","pdf",17859468,23,"English","# Abstract\n# Introduction\n# How to cite this article","[{\"question\":\"What is the study’s primary goal?\",\"answer\":\"To investigate and contrast circRNA expression patterns in cervical squamous carcinoma and adenocarcinoma, and to interpret their role in tumor differentiation mediated by HPV.\"},{\"question\":\"How were circRNA expression profiles obtained and validated?\",\"answer\":\"circRNA expression in SCC, ADC, ASC, and adjacent normal tissues was profiled by high-throughput RNA-seq, followed by bioinformatics analysis and qRT-PCR validation of sequencing results.\"},{\"question\":\"Which enriched pathway and key circRNAs were emphasized?\",\"answer\":\"The ubiquitin mediated proteolysis pathway was the most enriched across the three cervical cancer variants, and eight key candidate circRNAs derived from this pathway were further validated and used to build a ceRNA network.\"}]","Comprehensive profile and contrastive analysis of circular RNA expression in cervical squamous carcinoma and adenocarcinoma | PDF",1790269606,58]