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This study evaluates the prognostic value of the long non-coding RNA POLH-AS1 in HCC by analyzing RNA-Seq data from TCGA, measuring expression in HCC tissues and cells, and assessing associations with immune microenvironment features. Functional validation in HEP3B and HEPG2 shows that POLH-AS1 inhibition suppresses proliferation, invasion, and migration.",{"@graph":69,"@context":123},[70,84,106],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/comprehensive-analysis-of-polh-as1-as-a-prognostic-biomarker-in-hepatocellular-carcinoma/331271/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/comprehensive-analysis-of-polh-as1-as-a-prognostic-biomarker-in-hepatocellular-carcinoma/331271.png","ImageObject",300,407,{"name":92,"@type":93},"Theodore","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-26","2026-09-21",true,{"@type":102,"interactionType":103,"userInteractionCount":105},"InteractionCounter",{"@type":104},"ViewAction",12,{"@type":107,"mainEntity":108},"FAQPage",[109,115,119],{"name":110,"@type":111,"acceptedAnswer":112},"What clinical problem does the study address in hepatocellular carcinoma?","Question",{"text":113,"@type":114},"It addresses the poor outcomes and high mortality of HCC, where prognostic assessment and therapeutic guidance remain insufficient despite treatment advances.","Answer",{"name":116,"@type":111,"acceptedAnswer":117},"How is POLH-AS1 evaluated as a potential prognostic biomarker?",{"text":118,"@type":114},"The study uses RNA-Seq data from TCGA to analyze POLH-AS1 expression in HCC tissues and cells and relates expression levels to survival, prognosis, and immune microenvironment associations.",{"name":120,"@type":111,"acceptedAnswer":121},"What functional effects does POLH-AS1 inhibition have in HCC cells?",{"text":122,"@type":114},"Inhibition of POLH-AS1 reduces proliferation, invasion, and migration in HEP3B and HEPG2 HCC cell lines.","https://schema.org",{"og:url":83,"og:type":125,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":127,"canonical":83},"index,follow",{"doc_id":129,"site_id":62},331271,1790101450,{"code":4,"msg":5,"data":132},{"doc_id":129,"user_id":133,"nickname":92,"user_avatar":134,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":135,"file_id":136,"file_url":137,"file_type":138,"file_size":139,"view_count":105,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":105,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":41,"preview_page_count":4},7971461740886,"https://ap-avatar.wpscdn.com/davatar_3d24733baf745e90a7e4bdd5f77d97b2","Dong et al. BMC Cancer (2024) 24:1112 BMC Cancer  \n[https://doi.org/10.1186/s12885-024-12857-8](https://doi.org/10.1186/s12885-024-12857-8)  \nRESEARCH Open Access  \nComprehensive analysis of POLH-AS1 asa prognostic biomarker in hepatocellular carcinoma  \nYan Dong 1†, Xinyi Chen2†, ShenYang3†, Yilong Fu3, Liangyu Wang3, Xueping Gao4*, Di Chen5* and Lixia Xu3*  \nAbstract  \nBackground Hepatocellular carcinoma (HCC), a prevalent primary malignant tumor, is notorious for its high mortality rate. Despite advancements in HCC treatment, patient outcomes remain suboptimal. This study endeavors to assess the potential prognostic significance of POLH-AS1 in HCC.  \nMethods In this research, we gathered RNA-Seq information from individuals with HCC in The Cancer Genome Atlas (TCGA) . We analyzed the levels of POLH-AS1 expression in both HCC cells and tissues using statistical tests. Additionally, we examined various prognostic factors in HCC using advanced methodologies. Furthermore, we employed Spearman’s rank correlation analysis to examine the association between POLH-AS1 expression and the tumor’s immune microenvironment. Finally, the functional roles of POLH-AS1 in HCC were validated in two HCC cell lines (HEP3B and HEPG2) .  \nResults Our analysis revealed elevated POLH-AS1 expression across various cancers, including HCC, with heightened expression correlating with HCC progression. Notably, POLH-AS1 expression emerged as a potential biomarker for HCC patient survival and prognosis. Mechanistically, we identified the involvement of POLH-AS1 in tumorigenesis pathways such as herpes simplex virus 1 infection, interactions with neuroactive receptors, and the cAMP signaling pathway. Lastly, inhibition of POLH-AS1 was discovered to hinder the proliferation, invasion and migration of HEP3Band HEPG2 HCC cells.  \nConclusions POLH-AS1 emerges as a promising prognostic biomarker and therapeutic target for HCC, offering potential avenues for enhanced patient management and treatment strategies.  \nKeywords POLH-AS1, HCC, Biomarker, Survival, Prognosis  \n†Yan Dong, Xinyi Chen and ShenYang contributed equally to this work.  \n*Correspondence: Xueping Gao [gaoxueping95@163.com](gaoxueping95@163.com)[ ](gaoxueping95@163.com)Di Chen [mrdichen@163.com](mrdichen@163.com)[ ](mrdichen@163.com)Lixia Xu [mslixiaxu@163.com](mslixiaxu@163.com)  \n1Department of Hepatobiliary Surgery, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China  \n2Department of Gynecological Oncology, SunYat-sen University Cancer Center, Guangzhou, Guangdong, China  \n3Department of Infectious Diseases, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China  \n4Department of Laboratory Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China  \n5Department of Neurosurgery, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China  \n© The Author(s) 2024. Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit [http://](http://)[ ](http://)[creativecommons.org/l](creativecommons.org/l)icense","cbCaiuzObaBz5IPh","https://ap.wps.com/l/cbCaiuzObaBz5IPh","pdf",10423667,"English","# Abstract\n## Background\n## Methods\n## Results\n## Conclusions\n# Introduction","[{\"question\":\"What clinical problem does the study address in hepatocellular carcinoma?\",\"answer\":\"It addresses the poor outcomes and high mortality of HCC, where prognostic assessment and therapeutic guidance remain insufficient despite treatment advances.\"},{\"question\":\"How is POLH-AS1 evaluated as a potential prognostic biomarker?\",\"answer\":\"The study uses RNA-Seq data from TCGA to analyze POLH-AS1 expression in HCC tissues and cells and relates expression levels to survival, prognosis, and immune microenvironment associations.\"},{\"question\":\"What functional effects does POLH-AS1 inhibition have in HCC cells?\",\"answer\":\"Inhibition of POLH-AS1 reduces proliferation, invasion, and migration in HEP3B and HEPG2 HCC cell lines.\"}]","Comprehensive analysis of POLH-AS1 as a prognostic biomarker in hepatocellular carcinoma | PDF",1789992495]