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Duplicate mock forensic casework samples are prepared and randomly assigned to each platform, covering blood, saliva, cigarette butts, and touch DNA, including inhibited conditions from soil or oil substrates. Purified DNA is quantified with the Investigator Quantiplex Pro Kit and amplified with the Investigator 24plex QS Kit for STR analysis, including Quality Sensor data for extraction performance evaluation.",{"@graph":69,"@context":123},[70,84,106],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/comparison-of-two-dna-extraction-platforms-for-forensic-casework-applications-ez2-connect-fx-versus-maxwell-fsc-instrument-application-note/500330/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/comparison-of-two-dna-extraction-platforms-for-forensic-casework-applications-ez2-connect-fx-versus-maxwell-fsc-instrument-application-note/500330.png","ImageObject",300,407,{"name":92,"@type":93},"Chumphorn","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-16","2026-10-03",true,{"@type":102,"interactionType":103,"userInteractionCount":105},"InteractionCounter",{"@type":104},"ViewAction",13,{"@type":107,"mainEntity":108},"FAQPage",[109,115,119],{"name":110,"@type":111,"acceptedAnswer":112},"What two DNA extraction platforms are compared in this application note?","Question",{"text":113,"@type":114},"The EZ2 Connect Fx platform and the Maxwell FSC Instrument are compared for forensic casework DNA extraction.","Answer",{"name":116,"@type":111,"acceptedAnswer":117},"How are mock forensic casework samples prepared and assigned to platforms?",{"text":118,"@type":114},"Duplicate mock forensic casework samples are prepared, then randomly distributed for extraction by EZ2 Connect Fx or Maxwell FSC Instrument, with replicates used within each condition.",{"name":120,"@type":111,"acceptedAnswer":121},"How is DNA prepared for analysis after extraction?",{"text":122,"@type":114},"Purified DNA samples are quantified with the Investigator Quantiplex Pro Kit and amplified using the Investigator 24plex QS Kit for STR analysis.","https://schema.org",{"og:url":83,"og:type":125,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":127,"canonical":83},"index,follow",{"doc_id":129,"site_id":62},500330,1791093141,{"code":4,"msg":5,"data":132},{"doc_id":129,"user_id":133,"nickname":92,"user_avatar":134,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":135,"file_id":136,"file_url":137,"file_type":138,"file_size":139,"view_count":105,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":39,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":46},2336475401981,"https://ap-avatar.wpscdn.com/avatar/22000c94efd8d5204d?x-image-process=image/resize,m_fixed,w_180,h_180&k=1786935347598174694","Application Note  \nComparison of two DNA extraction platforms for use in forensic casework applications:  \nEZ2® Connect Fx versus Maxwell® FSC Instrument  \nVanessa Duchamp ¹, John Haley², Amy Liberty³, Ondrej Krsicka4, Nina Golob4, Lisa Dierig5 Authors’ affiliation(s)  \n1 QIAGEN Singapore, 8 Commonwealth Lane, \\#02-02, Singapore 149555  \n2 QIAGEN Manchester, 4 Skelton House, Lloyd Street North, Manchester, M15 6SH, UK  \n3 QIAGEN LLC, 19300 Germantown Road, Germantown, 20874, MD, USA  \n4 QIAGEN GmbH, QIAGEN Strasse 1, 40724 Hilden, Germany  \n5 Insitut für Rechtsmedizin, Universitätsklinikum Ulm, Albert-Einstein-Allee 23, 89081 Ulm, Germany  \nIntroduction  \nIn 2021, QIAGEN launched the EZ2 Connect Fx, anew instrument for end-to-end automation of nucleic acid extraction and purification in human identification applications. The EZ2 Connect Fx platform is based on the well-known and trusted EZ1® Advanced XL magnetic-bead purification technology.  \nPerformance of the EZ2 Connect Fx was compared with performance of an instrument regularly encountered in forensic laboratories, the Maxwell FSC Instrument (Promega Corporation) . Testing on the Maxwell FSC was performed at the Institute of Legal Medicine, Ulm and followed their validated workflow. Testing on the EZ2 Connect Fx instrument was undertaken by Lisa Dierig (ILM Ulm) in QIAGEN Hilden Research & Development Laboratory. Duplicate mock forensic casework samples were prepared in the Hilden laboratory and randomly distributed for extraction by the EZ2 Connect Fx or Maxwell FSC Instrument. All purified DNA samples were quantified with the Investigator® Quantiplex® Pro Kit and amplified with the Investigator 24plex QS Kit for STR analysis.  \nThis kit includes the innovative Quality Sensor, enabling additional data to be generated for quality control and evaluation of extraction performance.  \nMaterials and Methods  \nDNA samples  \nSamples were chosen to reflect real casework submissions: blood, saliva, cigarette butts and touch DNA samples. Liquid samples such as saliva and blood were pipettedonto a sterile glass slide and collected with a sterile moist swab, or a DNA-free swab previously lightly coated with soil or oil then allowed to dry. Some liquid samples were pipetted directly on to an inhibiting substrate.  \nSaliva samples  \nSaliva is a non-homogenous liquid. This can be problematic when saliva is pipetted and can lead to uneven cell distribution. Sample homogenization was achieved by adding nuclease-free water (1:1) to theneat saliva followed by thorough vortexing. This first dilution is referred to as “Saliva-Dil1”. Further dilutions were made as described below in Table 1. For each sample, 10 µl of diluted saliva was pipetted onto a glass slide then sampled by swabbing with a clean swab pre-wet with 10 µl of nuclease-free water. Four replicate swabs were made for each dilution; two were   \nSample to Insight  \nrandomly assigned to be processed by the EZ2 Connect Fx and two to be processed by the Maxwell FSC.  \nTable 1. Preparation of saliva samples  \nSample name Saliva dilution Method  \nSaliva-Dil1 Saliva: nuclease- Pipette 10 µl of Saliva-Dil1 on to a free water 1:1 slide then swab  \nSaliva-Dil2 Saliva-Dil1:  \nnuclease-free water 1:10  \nAdd 50 µl of Saliva-Dil1 to 450 µlof nuclease-free water  \nPipette 10 µl on to a slide  \nthen swab  \nSaliva-Dil3 Saliva-Dil2:  \nnuclease-free water 1:10  \nAdd 50 µl of Saliva-Dil2 to 450 µlof nuclease-free water  \nPipette 10 µl on to a slide then swab  \nBlood samples  \nBlood is a non-homogenous viscous liquid and may exhibit uneven cell distribution when it is pipetted. Sample homogenization was achieved by adding nuclease-free water (1:1) to the blood followed by thorough vortexing. This first dilution is referred to as “Blood-Dil1”. Further dilutions were made as described below in Table 2. For each dilution, 10 µl of blood sample was pipetted on toa glass slide then sampled by swabbing with a sterile swab pre-wet with 10 µl of nuclease-fre","cbCaiaaWn9EYEhZg","https://ap.wps.com/l/cbCaiaaWn9EYEhZg","pdf",353732,"English","# Introduction\n# Performance comparison setup\n# Materials and Methods\n## DNA samples\n## Saliva samples\n## Blood samples\n## Inhibited samples","[{\"question\":\"What two DNA extraction platforms are compared in this application note?\",\"answer\":\"The EZ2 Connect Fx platform and the Maxwell FSC Instrument are compared for forensic casework DNA extraction.\"},{\"question\":\"How are mock forensic casework samples prepared and assigned to platforms?\",\"answer\":\"Duplicate mock forensic casework samples are prepared, then randomly distributed for extraction by EZ2 Connect Fx or Maxwell FSC Instrument, with replicates used within each condition.\"},{\"question\":\"How is DNA prepared for analysis after extraction?\",\"answer\":\"Purified DNA samples are quantified with the Investigator Quantiplex Pro Kit and amplified using the Investigator 24plex QS Kit for STR analysis.\"}]","Comparison of two DNA extraction platforms for forensic casework applications - EZ2 Connect Fx versus Maxwell FSC Instrument - Application Note | PDF",1791010521]