[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-328469-105":59,"doc-detail-328469-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","comparing-length-and-telomere-expression-at-oral-precancerous-and-cancerous-stages","Comparing Length and Telomere Expression at Oral Precancerous and Cancerous Stages","","Telomeres are repetitive G-rich sequences at chromosome ends that serve as protein-binding regions, influencing genomic stability. This study compares telomere length and telomerase expression across blood, saliva, and tissue samples from patients spanning oral precancerous lesions to oral squamous cell carcinoma (OSCC). DNA was extracted and a TRAP assay used to measure telomerase activity, showing significant differences between OSCC and precancerous groups. Telomerase activity and telomere length support OSCC progression and offer diagnostic biomarker potential.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/comparing-length-and-telomere-expression-at-oral-precancerous-and-cancerous-stages/328469/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/comparing-length-and-telomere-expression-at-oral-precancerous-and-cancerous-stages/328469.png","ImageObject",300,407,{"name":92,"@type":93},"Oliver","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-22","2026-09-21",true,{"@type":102,"interactionType":103,"userInteractionCount":39},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What was the objective of the study?","Question",{"text":112,"@type":113},"To examine differences in telomere length in blood, saliva, and tissue samples at various stages of oral precancerous and cancerous lesions.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How were telomere length and telomerase expression assessed?",{"text":117,"@type":113},"DNA was extracted from collected blood, tissue, and saliva samples, and a TRAP assay was performed to assess and compare telomere length and telomerase expression.",{"name":119,"@type":110,"acceptedAnswer":120},"What were the key findings regarding telomerase activity?",{"text":121,"@type":113},"Telomerase activity levels differed significantly between OSCC patients and precancerous patients, indicating distinct telomerase behavior across stages.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},328469,1790095700,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":39,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":29,"language":139,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":140,"faqs":141,"seo_title":142,"seo_description":67,"update_tm":143,"read_time":144},8796095461610,"https://ap-avatar.wpscdn.com/davatar_276721f389ce27ea32af1340a28f341c","Iranian Journal of Pathology | ISSN: 2345-3656  \nComparing Length and Telomere Expression at Oral Precancerous  \nand Cancerous Stages  \nFahad Mansoor Samadi1, Shaista Suhail1, Manjari Sonam2, Mohd Kaleem Ahmad3, Vijay Kumar4,  \nShaleen Chandra1, Shadab Mohammad5  \n1. Department of Oral Pathology and Microbiology, King George Medical University, Lucknow, India  \n2. Department of Oral Pathology and Microbiology, Sardar Patel PG Institute of Dental and Medical Sciences, Lucknow, India  \n3. Department of Biochemistry, King George Medical University, Lucknow, India  \n4. Department of Surgical Oncology, King George Medical University, Lucknow, India  \n5. Department of Oral Maxillofacial Surgery, King George Medical University, Lucknow, India  \n\n| KEYWORDS |  |  | ABSTRACT |\n| --- | --- | --- | --- |\n| Carcinoma, Biomarkers, DNA, Prognosis, Telomere;\u003Cbr>Scan to discover online\u003Cbr>|  |  | Background & Objective: Telomeres consist of repetitive G-rich nucleotides located at the end of each chromosome, acting as protein binding sites. The aim of this study was to examine the differences in telomere length in blood, saliva, and tissue samples at various stages of oral precancerous and cancerous lesions.\u003Cbr>Methods: Samples of blood, tissue, and saliva were collected from patients with oral precancerous and cancerous lesions. DNA extraction was performed. Then, a TRAP assay was conducted to assess and compare the telomere length and telomerase expression.\u003Cbr>Results: The levels oftelomerase activity (TA) in the DNA samples ranged from 0.19 to 6.91 (2.05+ 1.37) among oral squamous cell carcinoma (OSCC) patients and from 0.17 to 4.5 (0.28+4.25) among precancerous patients. A significant difference was observed in TA levels between OSCC and precancerous samples (t=3.9691, P= 0.0000) .\u003Cbr>Conclusion: Assessing the telomerase activity is crucial for studying the behavior of carcinoma in the clinical setting. The augmented telomerase expression and the length of telomere contribute to OSCC progression. Hence, this study adds a diagnostic tool that can serve as a biomarker for the early detection and prognosis of OSCC. |\n| Main Subjects:\u003Cbr>Oral Pathology |  |  |  |\n| Received 16 Mar 2023; Accepted 22 Sep 2023; Published Online 15 Feb 2024; |  |  |  |\n|  10.30699/IJP.2024.1996330.3081 |  |  |  |\n| Corresponding Information: | Fahad Mansoor Samadi, Department of Oral Pathology and Microbiology, KGMU, Lucknow, India Email: [fahadmansoorsamadi@kgmcindia.edu](fahadmansoorsamadi@kgmcindia.edu) |  |  |\n| Copyright © 2024. This is an open-access article distributed under the terms of the Creative Commons Attribution-4.0 International License which permits Share, copy and redistribution of the material in any medium or format or adapt, remix, transform, and build upon the material for any purpose, even commercially. |  |  |  |\n\nIntroduction  \nTelomeres consist of G-rich nucleotides at the ends of every chromosome that repeat and act as binding sites for a wide range of proteins (1). These telomeres being specific assemblies encompass a tandem repeat sequence of particular DNA and related proteins. They have a well-defined protective function in averting genomic loss in the DNA sequence of the proliferating cells (2-4) . It has been established that with each cell division and due to certain oxidative stress reactions, these telomeres shorten in length and vary with cell type and age. Somatic cell telomeres range between 6 to 12 kb in size. In such instances, censoriously short telomeres instigate an apoptotic pathway or cellular senescence resulting in the cell division termination. For that reason, telomere shortening is considered an important mechanism for tumor suppression (5-9) . It has been documented that during cell division, each chromosome in normal cells loses approximately 50-100 base pairs at the 5' end (10) . This process acts like a biological clock, abolishing the surviving cells for longer periods to accrue mutations or aberrations (11,","cbCaieO3RhgGAoc4","https://ap.wps.com/l/cbCaieO3RhgGAoc4","pdf",376249,"English","# Background & Objective\n# Methods\n# Results\n# Conclusion\n# Introduction\n# Material and Methods","[{\"question\":\"What was the objective of the study?\",\"answer\":\"To examine differences in telomere length in blood, saliva, and tissue samples at various stages of oral precancerous and cancerous lesions.\"},{\"question\":\"How were telomere length and telomerase expression assessed?\",\"answer\":\"DNA was extracted from collected blood, tissue, and saliva samples, and a TRAP assay was performed to assess and compare telomere length and telomerase expression.\"},{\"question\":\"What were the key findings regarding telomerase activity?\",\"answer\":\"Telomerase activity levels differed significantly between OSCC patients and precancerous patients, indicating distinct telomerase behavior across stages.\"}]","Comparing Length and Telomere Expression at Oral Precancerous and Cancerous Stages | PDF",1789979913,15]