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It covers kit contents, storage conditions, intended use, and safety and quality control practices, then details the underlying principle of modified alkaline lysis and non-chaotropic binding chemistry. 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Our advanced, high-quality products and services ensure success from sample to result.  \nQIAGEN sets standards in:  \n􀂄 Purification of DNA, RNA, and proteins  \n􀂄 Nucleic acid and protein assays  \n􀂄 microRNA research and RNAi  \n􀂄 Automation of sample and assay technologies  \nOur mission is to enable you to achieve outstanding success and breakthroughs. For more information, [visit ](visit www.qiagen.com)[www.qiagen.com](visit www.qiagen.com).  \nContents  \nKit Contents 4  \nStorage 4  \nIntended Use 4  \nSafety Information 5  \nQuality Control 5  \nIntroduction 6  \nPrinciple and procedure 6  \nEquipment and Reagents to Be Supplied by User 8  \nImportant Notes 9  \nProtocol:  \n􀂄 Purification of Plasmid DNA using CompactPrep Plasmid Kits 15  \nTroubleshooting Guide 20  \nAppendix: Agarose Gel Analysis of the Purification Procedure 22  \nOrdering Information 24  \nKit Contents  \n\n| CompactPrep Plasmid Kit\u003Cbr>Catalog no. | Midi (25) 12843 | Maxi (25) 12863 |\n| --- | --- | --- |\n| CompactPrep Columns | 25 Midi Columns | 25 Maxi Columns |\n| Tube Extenders (10 ml) | 25 | – |\n| Tube Extenders (20 ml) | – | 25 |\n| Collection Tubes (2 ml) | 25 | 25 |\n| Buffer P1 | 2 x 50 ml | 3 x 50 ml |\n| Buffer P2 | 4 x 20 ml | 150 ml |\n| Buffer S3 | 70 ml | 2 x 70 ml |\n| Buffer BB | 70 ml | 2 x 70 ml |\n| Buffer PE (concentrate) | 6 ml | 6 ml |\n| Buffer EB | 15 ml | 15 ml |\n| RNase A* | 2 x 5 mg | 3 x 5 mg |\n| LyseBlue® | 2 x 50 µl | 3 x 50 µl |\n| Quick-Start Protocol | 1 | 1 |\n\n* Provided as a 10 mg/ml solution.  \nStorage  \nCompactPrep Plasmid Kits should be stored dry at room temperature (15–25°C) . Kits can be stored for up to 2 years without showing any reduction in performance and quality. After adding RNase A, Buffer P1 should be stored at 2–8°C and is stable for 6 months. Other buffers and RNase A stock solution can be stored for 2 years at room temperature.  \nIntended Use  \nCompactPrep Plasmid Kits are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.  \nAll due care and attention should be exercised in the handling of the products. We recommend all users of QIAGEN® products to adhere to the NIH guidelines that have been developed for recombinant DNA experiments, or to other applicable guidelines.  \nSafety Information  \nWhen working with chemicals, always wear a suitable lab coat, disposable gloves, and protective goggles. For more information, please consult the appropriate safety data sheets (SDSs) . These are available online in convenient and compact PDF format at www.qiagen.com/safety where you can find, view, and print the SDS for each QIAGEN kit and kit component.  \nQuality Control  \nIn accordance with QIAGEN’s ISO-certified Quality Management System, each lot of CompactPrep Plasmid Kit is tested against predetermined specifications to ensure consistent product quality.  \nIntroduction  \nQIAGEN CompactPrep Plasmid Kits provide a novel method for very fast (\u003C20 minutes) large-scale plasmid preparation, without the need for large volume centrifuges. The procedure is based on a novel, non-chaotropic binding chemistry, reducing the total preparation volume to miniprep scale. All protocol steps after cell harvest can be performed at the bench using QIAfilter cartridges, a vacuum manifold, and a standard microcentrifuge. CompactPrep Plasmid Kits do not contain QIAfilter cartridges and require lysate clearing by centrifugation. The unique kit chemistry and design of the CompactPrep column ensure largescale plasmid prep using a microspin column format.  \nCompactPrep Plasmid Kits provide molecular biology grade DNA, highly suited for routine applications such as sequencing, enz","cbCaitAZVoTKZ473","https://ap.wps.com/l/cbCaitAZVoTKZ473","pdf",381266,28,"English","# Kit Contents\n# Storage\n# Intended Use\n# Safety Information\n# Quality Control\n# Introduction\n# Principle and procedure\n# Equipment and Reagents to Be Supplied by User\n# Important Notes\n# Protocol: Purification of Plasmid DNA using CompactPrep Plasmid Kits\n# Troubleshooting Guide\n# Appendix: Agarose Gel Analysis of the Purification Procedure\n# Ordering Information","[{\"question\":\"What is the intended use of CompactPrep Plasmid Kits?\",\"answer\":\"CompactPrep Plasmid Kits are intended for molecular biology applications. They are not intended for the diagnosis, prevention, or treatment of a disease.\"},{\"question\":\"What principle does the CompactPrep protocol use?\",\"answer\":\"The protocol is based on a modified alkaline lysis procedure, followed by non-chaotropic binding chemistry using Buffer BB to bind plasmid DNA to the CompactPrep column membrane.\"},{\"question\":\"How is plasmid DNA obtained and prepared for use after elution?\",\"answer\":\"Cleared lysate is drawn through the CompactPrep column using a vacuum manifold. DNA is eluted by centrifugation in low volumes of Buffer EB, yielding highly concentrated DNA typically ready for immediate use without further alcohol precipitation.\"}]","CompactPrep Plasmid Purification Handbook - April 2012 | PDF",1790530731,71]