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These structural shifts are linked to substantial changes in microbial metabolic activity. AIM: Identify novel early diagnostic biomarkers for IBD using integrated multi-omics, assessing community structure and microbial metabolic profiles in pediatric and adult cohorts with ulcerative colitis (UC) and Crohn’s disease (CD). METHODS: Two age groups with confirmed diagnoses underwent MinION™ 16S rRNA sequencing and stool metabolomic profiling by GC/MS.",{"@graph":69,"@context":123},[70,84,106],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/combined-metabolomic-and-metagenomic-analysis-reveals-inflammatory-bowel-disease-diversity-in-pediatric-and-adult-patients-original-article-basic-study/406492/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/combined-metabolomic-and-metagenomic-analysis-reveals-inflammatory-bowel-disease-diversity-in-pediatric-and-adult-patients-original-article-basic-study/406492.png","ImageObject",300,407,{"name":92,"@type":93},"Stanley","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-29","2026-09-28",true,{"@type":102,"interactionType":103,"userInteractionCount":105},"InteractionCounter",{"@type":104},"ViewAction",11,{"@type":107,"mainEntity":108},"FAQPage",[109,115,119],{"name":110,"@type":111,"acceptedAnswer":112},"What is the main goal of the integrated multi-omics study?","Question",{"text":113,"@type":114},"To identify novel early diagnostic biomarkers for IBD by combining microbial community structure profiling with microbial metabolic activity profiling across pediatric and adult cohorts.","Answer",{"name":116,"@type":111,"acceptedAnswer":117},"How were microbial community structure and metabolic activity measured?",{"text":118,"@type":114},"16S rRNA gene sequencing was performed using the MinION™ Mk1B platform (MinKNOW software), and stool samples were analyzed using Shimadzu QP2010 Ultra GC/MS with a headspace extractor.",{"name":120,"@type":111,"acceptedAnswer":121},"What biomarkers or microbial signatures were reported as potentially diagnostic?",{"text":122,"@type":114},"Streptococcus salivarius and Escherichia coli were highlighted for assessing IBD risk in children, and five microbial signatures were identified with diagnostic potential for CD: Ralstonia insidiosa, Stenotrophomonas maltophilia, Erysipelatoclostridium ramosum, Blautia spp., and Coprococcus comes.","https://schema.org",{"og:url":83,"og:type":125,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":127,"canonical":83},"index,follow",{"doc_id":129,"site_id":62},406492,1790803318,{"code":4,"msg":5,"data":132},{"doc_id":129,"user_id":133,"nickname":92,"user_avatar":134,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":135,"file_id":136,"file_url":137,"file_type":138,"file_size":139,"view_count":105,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":140,"language":141,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":142,"faqs":143,"seo_title":144,"seo_description":67,"update_tm":145,"read_time":146,"preview_page_count":4},2336477405376,"https://ap-avatar.wpscdn.com/davatar_29158cc5080c5b710cf443261637dec0","| W | J | G | World Journal of\u003Cbr>Gastroenterology |\n| --- | --- | --- | --- |\n\nSubmit a Manuscript: [https://www.f6publishing.com](https://www.f6publishing.com) World J Gastroenterol 2025 December 28; 31(48): 112653  \nDOI: 10.3748/wjg.v31.i48.112653 ISSN 1007-9327 (print) ISSN 2219-2840 (online)  \nORIGINAL ARTICLE  \nBasic Study  \nCombined metabolomic and metagenomic analysis reveals  \ninflammatory bowel disease diversity in pediatric and adult patients  \nNatalya B Zakharzhevskaya, Svetlana I Erdes, Elena A Belousova, Ivan S Samolygo, Marina A Manina, Polina V Kondrashova, Ekaterina Y Lomakina, Dmitry A Kardonsky, Elizaveta A Vorobyeva, Olga Y Shagaleeva, Artemiy A Silantyev, Victoriia D Kazakova, Daria A Kashatnikova, Tatiana N Kalachnuk, Irina V Kolesnikova, Andrey V Chaplin, Maria I Markelova, Tatiana V Grigoryeva, Evgenii I Olekhnovich, Vladimir A Veselovsky, Maxim D Morozov, Polina Y Zoruk, Daria I Boldyreva, Anna A Vanyushkina, Boris A Efimov  \nSpecialty type: Gastroenterology and hepatology  \nProvenance and peer review:  \nUnsolicited article; Externally peer reviewed.  \nPeer-review model: Single blind  \nPeer-review report’s classification Scientific Quality: Grade A, Grade C  \nNovelty: Grade B, Grade B  \nCreativity or Innovation: Grade B, Grade B  \nScientific Significance: Grade A, Grade B  \nP-Reviewer: Alshimerry AF, PhD, Assistant Professor, Iraq; Li P, MD, PhD, Chief Physician, Director, Professor, Senior Researcher, China  \nReceived: August 4, 2025  \nRevised: September 9, 2025  \nAccepted: November 11, 2025  \nPublished online: December 28, 2025  \nProcessing time: 147 Days and 16.1 Hours  \nNatalya B Zakharzhevskaya, Dmitry A Kardonsky, Elizaveta A Vorobyeva, Olga Y Shagaleeva, Artemiy A Silantyev, Victoriia D Kazakova, Daria A Kashatnikova, Irina V Kolesnikova, Vladimir AVeselovsky, Boris A Efimov, Department of Postgenomics, Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow 119435, Russia  \nSvetlana I Erdes, Ivan S Samolygo, Marina A Manina, Department of Propaedeutics of Children’s Diseases, N.F. Filatov Clinical Institute of Children’s Health, I.M. Sechenov First Moscow State Medical University, Moscow 119435, Russia  \nElena A Belousova, Ekaterina Y Lomakina, Department of Gastroenterology, M.F. Vladimirskiy Moscow Regional Research and Clinical Institute, Moscow 129110, Russia  \nPolina V Kondrashova, Department of Gastroenterology, Children’s City polyclinic No. 81 of the Moscow Department of Health, Moscow 117342, Russia  \nDaria A Kashatnikova, The Laboratory of Ecological Genetics, Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow 119991, Russia  \nTatiana N Kalachnuk, Department of Gastroenterology, Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow 119435, Russia  \nAndrey V Chaplin, Boris A Efimov, Department of Microbiology and Virology, Pirogov Russian National Research Medical University, Moscow 117997, Russia  \nMaria I Markelova, Tatiana V Grigoryeva, Department of Proteomics, Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, Kazan 420008, Russia  \nEvgenii I Olekhnovich, Maxim D Morozov, Polina Y Zoruk, Daria I Boldyreva, Department of System Biology, Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow 119435, Russia  \nAnna A Vanyushkina, Vladimir Zelman Center for Neurobiology and Brain Rehabilitation, Skolkovo Institute of Science and Technology, Moscow 121205, Russia  \n WJG  [https://www.wjgnet.com](https://www.wjgnet.com) 1 December 28, 2025  Volume 31  Issue 48   \nZakharzhevskaya NB et al. Functional metabolomics of IBD in pediatric patients  \nCorresponding author: Natalya B Zakharzhevskaya, PhD, Postdoc, Department of Postgenomics, Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biologic","cbCaib5sHw8z6kge","https://ap.wps.com/l/cbCaib5sHw8z6kge","pdf",18330864,26,"English","# Abstract\n## Background\n## Aim\n## Methods\n## Results","[{\"question\":\"What is the main goal of the integrated multi-omics study?\",\"answer\":\"To identify novel early diagnostic biomarkers for IBD by combining microbial community structure profiling with microbial metabolic activity profiling across pediatric and adult cohorts.\"},{\"question\":\"How were microbial community structure and metabolic activity measured?\",\"answer\":\"16S rRNA gene sequencing was performed using the MinION™ Mk1B platform (MinKNOW software), and stool samples were analyzed using Shimadzu QP2010 Ultra GC/MS with a headspace extractor.\"},{\"question\":\"What biomarkers or microbial signatures were reported as potentially diagnostic?\",\"answer\":\"Streptococcus salivarius and Escherichia coli were highlighted for assessing IBD risk in children, and five microbial signatures were identified with diagnostic potential for CD: Ralstonia insidiosa, Stenotrophomonas maltophilia, Erysipelatoclostridium ramosum, Blautia spp., and Coprococcus comes.\"}]","Combined metabolomic and metagenomic analysis reveals inflammatory bowel disease diversity in pediatric and adult patients - ORIGINAL ARTICLE - Basic Study | PDF",1790566067,66]