[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-270657-105":59,"doc-detail-270657-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","collection-of-blood-from-wildlife-policy-sa-dew","Collection of blood from wildlife policy (SA DEW)","","This standard operating procedure provides researchers with guidance for designing wildlife research proposals that use blood collection techniques approved by the Wildlife Ethics Committee. It explains how suitable blood collection methods depend on species, age, sex, required blood volume, and study needs, and notes that non-recommended methods may delay assessment. The document sets limits for maximum blood volume and DNA sampling, emphasizes the Three R’s, and outlines practical considerations for haemostasis, puncture-site preparation, and unacceptable collection approaches.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/collection-of-blood-from-wildlife-policy-sa-dew/270657/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/collection-of-blood-from-wildlife-policy-sa-dew/270657.png","ImageObject",300,407,{"name":92,"@type":93},"Clementine","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-08","2026-09-15",true,{"@type":102,"interactionType":103,"userInteractionCount":52},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What is the purpose of the Collection of blood from wildlife policy (SA DEW) SOP?","Question",{"text":112,"@type":113},"It helps researchers design proposals using blood collection techniques generally approved by the Wildlife Ethics Committee, and it provides the guidelines used to review applications.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"What is the recommended maximum blood volume for a single sample?",{"text":117,"@type":113},"The recommended maximum is 10% of the circulating blood volume, and as a general guide this corresponds to about 1% of the total body weight.",{"name":119,"@type":110,"acceptedAnswer":120},"Which blood collection methods are unacceptable under this SOP?",{"text":121,"@type":113},"The SOP states that retro-orbital bleeding (with recovery) is not approved except under tightly argued exceptional circumstances, and using the footpad is unacceptable due to sensitivity and infection risk.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},270657,1789456244,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":52,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":129,"read_time":41},1374391974564,"https://ap-avatar.wpscdn.com/avatar/14000253aa45c000a9e?x-image-process=image/resize,m_fixed,w_180,h_180&k=1779874745381141002","Charles Darwin University Animal Ethics Committee  \nStandard Operating Procedure:  \nCollection of blood from wildlife policy (SA DEW)  \n\n| Version No: | Version 1 |\n| --- | --- |\n| Date of Approval by the CDU AEC: | 05/03/2025 |\n| Date for Review by the CDU AEC: | 05/03/2028 |\n\nNOTE:  \n• Crocodile blood collection techniques are not referenced in this SOP. If this is to occur, procedures should be outlined in your project application.  \nCollection of blood from wildlife policy  \nIntroduction1  \nThe purpose of this document is to provide researchers with information that will assist in designing research proposals that use techniques generally approved by the Wildlife Ethics Committee (WEC) . These are the guidelines against which your application will be reviewed. This document includes suitable methods of blood collection procedures for a selection of animal species (native and exotic) that could be used in wildlife studies. The emphasis is not on practical detail, as researchers must be able to demonstrate a suitable level of knowledge, training and experience.  \nThe most appropriate methods of blood collection will vary according to the species, individual animal characteristics (e.g. age, sex), the volume of blood that is required, and the requirements of the research. A number of recommended methods are suggested but this does not preclude the use of other techniques, which may be justified on a case-by-case basis. However, if you apply to use methods that are not recommended by the WEC, your application may take longer to assess.  \nMethods which may have been in common use in the past but are now considered controversial, undesirable or unsatisfactory on an animal welfare basis are mentioned.  \nBlood volume 2,3,4  \nThe recommended maximum volume of blood collected as a single sample is 10% of the circulating blood volume. As a general guide, the circulating blood volume of most animals is approximately 5- 10% of the animal’s bodyweight. Thus a maximum of 1% of the total body weight is generally the maximum recommended volume of blood to be collected.  \nOnly small amounts of blood are needed for most DNA studies. The following are the maximum allowable volumes for DNA studies5. Applicants will need to clearly justify why amounts larger than this are needed.  \nAnimals up to 7g  \nAnimals 7-15g  \nAnimals over 15g  \n-1/3 standard capillary tube  \n-1/2 standard capillary tube  \n-1 standard capillary tube  \n(A standard capillary tube is defined as a tube that holds 60 microlitres)  \nThree R’s – Refine, Reduce, Replace  \nBlood collection impacts upon the pain and distress experienced by the animal, therefore refinements to the methodology must be considered in order to meet the requirements of the Australian Code of Practice for the care and use of animals for scientific purposes (2004)6.  \nArrangements should be made to ensure that any surplus materials can be shared with other researchers by lodgement at a suitable Institution (such as the Evolutionary Biology Unit of the South Australian Museum)– this can reduce future sampling. Blood sampling should only be an option where other tissues, that can be collected less invasively (e.g. feathers, fur, scale clippings), are not a suitable replacement.  \nThese points should be addressed in your application.  \nHaemostasis  \nIn all cases, haemostasis and arrangements for monitoring of bleeding post-procedure must be carefully planned. Pressure should be applied immediately following withdrawal of the needle, or when sufficient blood has been obtained from a needle-prick. Aids to control excessive bleeding should also be readily available (e.g. antiseptic powders, cornstarch or tissue glue) . Silver nitrate sticks (styptic pencils) are caustic and should not be used on skin.  \nPreparation of the puncture site  \nDiluted chlorhexidine solution (1:40) can be used to prepare the puncture site. Alcohol is generally suitable, but as it is an irritant, should not be used on frogs. Geckoes and","cbCaivTiEFcFwuIQ","https://ap.wps.com/l/cbCaivTiEFcFwuIQ","pdf",1014045,12,"English","# Charles Darwin University Animal Ethics Committee - Standard Operating Procedure\n## Collection of blood from wildlife policy (SA DEW)\n## Introduction\n## Blood volume\n## Three R’s – Refine, Reduce, Replace\n## Haemostasis\n## Preparation of the puncture site\n## Unacceptable methods\n## Recommended Species Specific Sites for Blood Collection\n## MAMMALS","[{\"question\":\"What is the purpose of the Collection of blood from wildlife policy (SA DEW) SOP?\",\"answer\":\"It helps researchers design proposals using blood collection techniques generally approved by the Wildlife Ethics Committee, and it provides the guidelines used to review applications.\"},{\"question\":\"What is the recommended maximum blood volume for a single sample?\",\"answer\":\"The recommended maximum is 10% of the circulating blood volume, and as a general guide this corresponds to about 1% of the total body weight.\"},{\"question\":\"Which blood collection methods are unacceptable under this SOP?\",\"answer\":\"The SOP states that retro-orbital bleeding (with recovery) is not approved except under tightly argued exceptional circumstances, and using the footpad is unacceptable due to sensitivity and infection risk.\"}]","Collection of blood from wildlife policy (SA DEW) | PDF"]