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Exosomal levels correlate with clinicopathological characteristics, suggesting biomarker potential and regulatory roles in NSCLC.",{"@graph":14,"@context":73},[15,34,56],{"@type":16,"itemListElement":17},"BreadcrumbList",[18,23,27,31],{"item":19,"name":20,"@type":21,"position":22},"https://docshare.wps.com","Home","ListItem",1,{"item":24,"name":25,"@type":21,"position":26},"https://docshare.wps.com/document/","Document",2,{"item":28,"name":29,"@type":21,"position":30},"https://docshare.wps.com/document/research-report/","Research & Report",3,{"item":32,"name":10,"@type":21,"position":33},"https://docshare.wps.com/document/circulating-exosomal-mirna-profiles-in-non-small-cell-lung-cancers/333859/",4,{"url":32,"name":10,"@type":35,"image":36,"author":41,"headline":10,"publisher":44,"fileFormat":47,"inLanguage":8,"description":12,"dateModified":48,"datePublished":49,"encodingFormat":47,"isAccessibleForFree":50,"interactionStatistic":51},"DigitalDocument",{"url":37,"@type":38,"width":39,"height":40},"https://docshare.wps.com/thumbnails/circulating-exosomal-mirna-profiles-in-non-small-cell-lung-cancers/333859.png","ImageObject",300,407,{"name":42,"@type":43},"Levi","Person",{"url":19,"name":45,"@type":46},"DocShare","Organization","application/pdf","2026-11-07","2026-09-21",true,{"@type":52,"interactionType":53,"userInteractionCount":55},"InteractionCounter",{"@type":54},"ViewAction",10,{"@type":57,"mainEntity":58},"FAQPage",[59,65,69],{"name":60,"@type":61,"acceptedAnswer":62},"What was the main goal of the study?","Question",{"text":63,"@type":64},"To evaluate the exosomal miRNA content in plasma exosomes from ostensibly healthy individuals versus patients with SQCLC or LUAD, and assess whether specific circulating exosomal miRNAs can relate to NSCLC biology and clinical characteristics.","Answer",{"name":66,"@type":61,"acceptedAnswer":67},"How many circulating exosomal miRNAs were profiled, and which miRNAs were selected for deeper analysis?",{"text":68,"@type":64},"The study examined 25 circulating exosomal miRNAs. Eight miRNAs—miR-21-5p, miR-126-3p, miR-210-3p, miR-221-3p, Let-7b-5p, miR-146a-5p, miR-222-3p, and miR-9-5p—were highly enriched and selected for further analyses.",{"name":70,"@type":61,"acceptedAnswer":71},"Did the selected miRNAs show signals across cancer stages and patient sex?",{"text":72,"@type":64},"Yes. The eight miRNAs were readily detected in NSCLC patients of both sexes at all cancer stages, and their exosomal levels correlated with clinicopathological characteristics of the tumors.","https://schema.org",{"og:url":32,"og:type":75,"og:title":10,"og:site_name":45,"og:description":12},"article",{"robots":77,"canonical":32},"index,follow",{"doc_id":79,"site_id":7},333859,1790319509,{"code":4,"msg":82,"data":83},"success",[84,88,92,96,101,106,111,115,120,123,126],{"id":22,"doc_module":4,"doc_module_name":25,"category_name":85,"show_sort_weight":86,"slug":87},"Story & Novel",90,"story-novel",{"id":26,"doc_module":4,"doc_module_name":25,"category_name":89,"show_sort_weight":90,"slug":91},"Literature",80,"literature",{"id":33,"doc_module":4,"doc_module_name":25,"category_name":93,"show_sort_weight":94,"slug":95},"Exam",70,"exam",{"id":97,"doc_module":4,"doc_module_name":25,"category_name":98,"show_sort_weight":99,"slug":100},5,"Comic",60,"comic",{"id":102,"doc_module":4,"doc_module_name":25,"category_name":103,"show_sort_weight":104,"slug":105},6,"Technology",50,"technology",{"id":107,"doc_module":4,"doc_module_name":25,"category_name":108,"show_sort_weight":109,"slug":110},7,"Healthcare",40,"healthcare",{"id":112,"doc_module":4,"doc_module_name":25,"category_name":29,"show_sort_weight":113,"slug":114},8,30,"research-report",{"id":116,"doc_module":4,"doc_module_name":25,"category_name":117,"show_sort_weight":118,"slug":119},9,"Religion & Spirituality",20,"religion-spirituality",{"id":118,"doc_module":4,"doc_module_name":25,"category_name":121,"show_sort_weight":118,"slug":122},"World Cup","world-cup",{"id":55,"doc_module":4,"doc_module_name":25,"category_name":124,"show_sort_weight":55,"slug":125},"Lifestyle","lifestyle",{"id":127,"doc_module":4,"doc_module_name":25,"category_name":128,"show_sort_weight":97,"slug":129},19,"General","general",{"code":4,"msg":82,"data":131},{"doc_id":79,"user_id":132,"nickname":42,"user_avatar":133,"doc_module":4,"category_id":112,"category_name":29,"doc_title":10,"doc_description":12,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":55,"is_deleted":4,"is_public":22,"is_downloadable":22,"audit_status":22,"page_count":139,"language":140,"language_code":8,"site_id":7,"html_lang":8,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":12,"update_tm":144,"read_time":145,"preview_page_count":4},7971461740909,"https://ap-avatar.wpscdn.com/davatar_155a257f0dc6eb9ab79c44ca47cae57d","cells  \nArticle  \nCirculating Exosomal miRNA Profiles in Non-Small Cell Lung Cancers  \nAbeer A. I. Hassanin 1,2 and Kenneth S. Ramos 1, *  \nCitation: Hassanin, A.A.I.; Ramos, K.S. Circulating Exosomal miRNA Profiles in Non-Small Cell Lung Cancers. Cells 2024, 13, 1562 . [https://doi.org/10.3390/](https://doi.org/10.3390/)[ ](https://doi.org/10.3390/)cells13181562  \nAcademic Editor: Riccardo Di Santo  \nReceived: 22 August 2024  \nRevised: 8 September 2024  \nAccepted: 15 September 2024  \nPublished: 17 September 2024  \nCopyright: © 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license ([https://](https://)[ ](https://)[creativecommons.org/licenses/by/](creativecommons.org/licenses/by/)[ ](creativecommons.org/licenses/by/)[4.0/](4.0/)) .  \n1 Center for Genomic and Precision Medicine, Texas A&M Institute of Biosciences and Technology, Texas Medical Center, Houston, TX 77030, USA; [ahassanin@tamu.edu](ahassanin@tamu.edu)  \n2 Department of Animal Wealth Development, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt  \n* Correspondence: [kramos@tamu.edu](kramos@tamu.edu)  \nAbstract: A growing number of studies have shown that microRNAs (miRNAs) can exert oncogenicor tumor suppressor activities in a variety of cancers, including lung cancer. Given their presence in exosome preparations, microRNA molecules may in fact participate in exosomal intercellular transfers and signaling. In the present study, we examined the profile of 25 circulating exosomal microRNAs in ostensibly healthy controls compared to patients with squamous cell lung cancers (SQCLC) or lung adenocarcinomas (LUAD) . Eight miRNAs, namely, miR-21-5p, miR-126-3p, miR- 210-3p, miR-221-3p, Let-7b-5p, miR-146a-5p, miR-222-3p, and miR-9-5p, were highly enriched in the cohort and selected for further analyses. All miRNAs were readily detected in non-small cell lung cancer (NSCLC) patients of both sexes at all cancer stages, and their levels in exosomes correlated with the clinicopathological characteristics of tumors. Thus, the presence of these miRNAs in circulating exosomes may contribute to the regulation of oncogenic activity in patients with NSCLC.  \nKeywords: exosomes; miRNAs; NSCLC; diagnostic performance; gene ontology  \n1. Introduction  \nDespite extensive research conducted over the past two decades, cancer continues to be a dominant cause of death, ranking as the second highest cause of mortality in the United States [1,2] . Global cancer statistics in 2024 show that lung cancer accounts for the second highest number of cancer-related deaths, with over 340 individuals dying daily from this disease [3] . Therefore, the development of reliable and effective tools to evaluate lung cancer continues to be a priority.  \nExosomes are small membrane-bound vesicles that contain proteins, RNAs and DNA fragments implicated in intercellular communication in cancer [4,5] . These vesicles range in diameter from 30 to 150 nanometers and form early multivesicular bodies through sprouting that upon fusion with the plasma membrane create an intracellular vesicle that is subsequently discharged into the extracellular milieu [4,6] . The molecular composition of exosomes reflects the intracellular condition of their source cells, with mounting evidence showing that the intracellular state of cancer-derived exosomes is strikingly the same as that of their mother cells [7,8] .  \nMicroRNAs (miRNAs) are short sequences of non-coding RNA (20–24 nucleotides) that play crucial regulatory functions in both unicellular and multicellular eukaryotes [9,10] . miRNAs can recognize and bind to complementary sites in the 3′ untranslated region of the target mRNA, resulting in post-transcriptional gene silencing through degradation of the target or inhibition of translation, depending on the degree of complementarity between the miRNA and the ta","cbCaiqINTohjLuAo","https://ap.wps.com/l/cbCaiqINTohjLuAo","pdf",9986223,17,"English","# 1. Introduction\n## Exosomes in cancer and intercellular communication\n## MicroRNAs and regulatory roles in cancer\n# 2. Material and Methods\n## Exosome Isolation and Characterization\n## Plasma Samples and Patients’ Clinical Characteristics","[{\"question\":\"What was the main goal of the study?\",\"answer\":\"To evaluate the exosomal miRNA content in plasma exosomes from ostensibly healthy individuals versus patients with SQCLC or LUAD, and assess whether specific circulating exosomal miRNAs can relate to NSCLC biology and clinical characteristics.\"},{\"question\":\"How many circulating exosomal miRNAs were profiled, and which miRNAs were selected for deeper analysis?\",\"answer\":\"The study examined 25 circulating exosomal miRNAs. Eight miRNAs—miR-21-5p, miR-126-3p, miR-210-3p, miR-221-3p, Let-7b-5p, miR-146a-5p, miR-222-3p, and miR-9-5p—were highly enriched and selected for further analyses.\"},{\"question\":\"Did the selected miRNAs show signals across cancer stages and patient sex?\",\"answer\":\"Yes. The eight miRNAs were readily detected in NSCLC patients of both sexes at all cancer stages, and their exosomal levels correlated with clinicopathological characteristics of the tumors.\"}]","Circulating Exosomal miRNA Profiles in Non-Small Cell Lung Cancers | PDF",1790004350,43]