[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"doc-seo-341805-105":3,"detail-sidebar-cat-0-en-105":81,"doc-detail-341805-en":131},{"code":4,"msg":5,"data":6},0,"ok",{"site_id":7,"language":8,"slug":9,"title":10,"keywords":11,"description":12,"schema_data":13,"social_meta":74,"head_meta":76,"extra_data":78,"updated_unix":80},105,"en","cht-ceramic-hydroxyapatite-instruction-manual","CHT™ Ceramic Hydroxyapatite - Instruction Manual","","CHT™ Ceramic Hydroxyapatite instruction manual covering introduction and product overview, including what the ceramic hydroxyapatite is, key specifications, and handling and packing guidance. The manual details the chromatography mechanism, buffers and tabled stability in various buffers, elution considerations, trace metal contamination, phosphate and calcium behavior, chemical compatibility and load preparation. It further provides method development protocols for IgG monoclonal antibodies, globular proteins, plasmids, and acidic proteins, plus regeneration, sanitization, storage, and detailed column packing protocols.",{"@graph":14,"@context":73},[15,34,56],{"@type":16,"itemListElement":17},"BreadcrumbList",[18,23,27,31],{"item":19,"name":20,"@type":21,"position":22},"https://docshare.wps.com","Home","ListItem",1,{"item":24,"name":25,"@type":21,"position":26},"https://docshare.wps.com/document/","Document",2,{"item":28,"name":29,"@type":21,"position":30},"https://docshare.wps.com/document/healthcare/","Healthcare",3,{"item":32,"name":10,"@type":21,"position":33},"https://docshare.wps.com/document/cht-ceramic-hydroxyapatite-instruction-manual/341805/",4,{"url":32,"name":10,"@type":35,"image":36,"author":41,"headline":10,"publisher":44,"fileFormat":47,"inLanguage":8,"description":12,"dateModified":48,"datePublished":49,"encodingFormat":47,"isAccessibleForFree":50,"interactionStatistic":51},"DigitalDocument",{"url":37,"@type":38,"width":39,"height":40},"https://docshare.wps.com/thumbnails/cht-ceramic-hydroxyapatite-instruction-manual/341805.png","ImageObject",300,407,{"name":42,"@type":43},"Bintang","Person",{"url":19,"name":45,"@type":46},"DocShare","Organization","application/pdf","2026-10-29","2026-09-22",true,{"@type":52,"interactionType":53,"userInteractionCount":55},"InteractionCounter",{"@type":54},"ViewAction",11,{"@type":57,"mainEntity":58},"FAQPage",[59,65,69],{"name":60,"@type":61,"acceptedAnswer":62},"What should be reviewed before using CHT™ ceramic hydroxyapatite?","Question",{"text":63,"@type":64},"Review the instructions prior to use and consult your local Bio-Rad Laboratories sales representative if you have questions or comments about the guidance.","Answer",{"name":66,"@type":61,"acceptedAnswer":67},"What topics are included in the chromatography section?",{"text":68,"@type":64},"It covers the chromatography mechanism, buffer considerations (including stability in various buffers), elution, trace metal contamination, phosphate and calcium behavior, chemical compatibility and load preparation, and method development protocols.",{"name":70,"@type":61,"acceptedAnswer":71},"How does the manual guide regeneration, sanitization, and storage?",{"text":72,"@type":64},"It provides dedicated sections for regeneration, sanitization, and storage, along with specific column packing protocols to support safe and consistent reuse and handling.","https://schema.org",{"og:url":32,"og:type":75,"og:title":10,"og:site_name":45,"og:description":12},"article",{"robots":77,"canonical":32},"index,follow",{"doc_id":79,"site_id":7},341805,1790619270,{"code":4,"msg":82,"data":83},"success",[84,88,92,96,101,106,110,115,120,123,127],{"id":22,"doc_module":4,"doc_module_name":25,"category_name":85,"show_sort_weight":86,"slug":87},"Story & Novel",90,"story-novel",{"id":26,"doc_module":4,"doc_module_name":25,"category_name":89,"show_sort_weight":90,"slug":91},"Literature",80,"literature",{"id":33,"doc_module":4,"doc_module_name":25,"category_name":93,"show_sort_weight":94,"slug":95},"Exam",70,"exam",{"id":97,"doc_module":4,"doc_module_name":25,"category_name":98,"show_sort_weight":99,"slug":100},5,"Comic",60,"comic",{"id":102,"doc_module":4,"doc_module_name":25,"category_name":103,"show_sort_weight":104,"slug":105},6,"Technology",50,"technology",{"id":107,"doc_module":4,"doc_module_name":25,"category_name":29,"show_sort_weight":108,"slug":109},7,40,"healthcare",{"id":111,"doc_module":4,"doc_module_name":25,"category_name":112,"show_sort_weight":113,"slug":114},8,"Research & Report",30,"research-report",{"id":116,"doc_module":4,"doc_module_name":25,"category_name":117,"show_sort_weight":118,"slug":119},9,"Religion & Spirituality",20,"religion-spirituality",{"id":118,"doc_module":4,"doc_module_name":25,"category_name":121,"show_sort_weight":118,"slug":122},"World Cup","world-cup",{"id":124,"doc_module":4,"doc_module_name":25,"category_name":125,"show_sort_weight":124,"slug":126},10,"Lifestyle","lifestyle",{"id":128,"doc_module":4,"doc_module_name":25,"category_name":129,"show_sort_weight":97,"slug":130},19,"General","general",{"code":4,"msg":82,"data":132},{"doc_id":79,"user_id":133,"nickname":42,"user_avatar":134,"doc_module":4,"category_id":107,"category_name":29,"doc_title":10,"doc_description":12,"doc_content":135,"file_id":136,"file_url":137,"file_type":138,"file_size":139,"view_count":55,"is_deleted":4,"is_public":22,"is_downloadable":22,"audit_status":22,"page_count":140,"language":141,"language_code":8,"site_id":7,"html_lang":8,"table_of_contents":142,"faqs":143,"seo_title":144,"seo_description":12,"update_tm":145,"read_time":146},962085564381,"https://ap-avatar.wpscdn.com/davatar_6f874abed73319feea01a86fa6f0fab8","CHT™ Ceramic Hydroxyapatite  \nInstruction Manual  \nPlease read these instructions prior to using CHT ceramic hydroxyapatite. If you have any questions or comments regarding these instructions, please contact your local Bio-Rad Laboratories sales representative.  \nTable of Contents  \nSection 1 Introduction ...............................................................................................................................................1  \nSection 2 Product Description...................................................................................................................................1  \n2.1 What is CHT™ Ceramic Hydroxyapatite?................................................................................................................1  \n2.2 Specifications..........................................................................................................................................................2  \n2.3 Characteristics ........................................................................................................................................................2  \n2.4 General Handling and Packing ................................................................................................................................3  \nSection 3 Chromatography ........................................................................................................................................3  \n3.1 CHT™ Ceramic Hydroxyapatite Mechanism ...........................................................................................................3  \n3.1 .1 Buffers .......................................................................................................................................................4  \nTable I: CHT Stability in Various Buffers ......................................................................................................4  \n3.1 .2 Elution .......................................................................................................................................................4  \n3.1.3 Trace Metal Contamination ........................................................................................................................5  \n3.1 .4 Phosphate .................................................................................................................................................5  \n3.1 .5 Calcium .....................................................................................................................................................5  \n3.1.6 Chemical Compatibility/Load Preparation...................................................................................................5  \n3.2 Method Development..............................................................................................................................................6  \n3.2.1 Protocol I: IgG Monoclonal Antibodies .......................................................................................................8  \n3.2.2 Protocol II: Globular Proteins....................................................................................................................10  \n3.2.3 Protocol III: Plasmids................................................................................................................................12  \n3.2.4 Protocol IV: Acidic Proteins ......................................................................................................................14  \n3.2.5 Scouting Tips........................................................................................................................................... 14  \nSection 4 Regeneration, Sanitization, and Storage .................................................................................................15  \n4.1 Regeneration .........................................................................................................................................","cbCaiah4qA1Mxvi7","https://ap.wps.com/l/cbCaiah4qA1Mxvi7","pdf",347560,43,"English","# Section 1 Introduction\n# Section 2 Product Description\n## 2.1 What is CHT™ Ceramic Hydroxyapatite?\n## 2.2 Specifications\n## 2.3 Characteristics\n## 2.4 General Handling and Packing\n# Section 3 Chromatography\n## 3.1 CHT™ Ceramic Hydroxyapatite Mechanism\n## 3.2 Method Development\n# Section 4 Regeneration, Sanitization, and Storage\n## 4.1 Regeneration\n## 4.2 Sanitization\n## 4.3 Storage\n# Section 5 Column Packing Protocols\n## 5.1 General Handling and Powder Preparation\n## 5.2 Guidelines for Packing Low-Pressure Process Columns\n## 5.3 Open-Column Methods\n## 5.4 Media Transfer Station Methods\n## 5.5 Media Packing Station Methods","[{\"question\":\"What should be reviewed before using CHT™ ceramic hydroxyapatite?\",\"answer\":\"Review the instructions 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