[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"doc-seo-333080-105":3,"doc-detail-333080-en":81,"detail-sidebar-cat-0-en-105":99},{"code":4,"msg":5,"data":6},0,"ok",{"site_id":7,"language":8,"slug":9,"title":10,"keywords":11,"description":12,"schema_data":13,"social_meta":74,"head_meta":76,"extra_data":78,"updated_unix":80},105,"en","characterization-of-m6a-methylation-modifications-in-gastric-cancer","Characterization of m6A methylation modifications in gastric cancer","","Widely recognized as an essential epitranscriptomic modification, RNA N6-methyladenosine (m6A) regulates physiological and pathological processes. This study examines m6A modification roles in gastric cancer using TCGA-STAD and GEO datasets (GSE84426, GSE84433). Based on 18 m6A regulators, m6A modification patterns were profiled, and an m6Ascore was constructed via principal component analysis to evaluate links with tumor somatic mutations and immune checkpoint blockade therapy. Validation in 34 samples identified three m6A patterns.",{"@graph":14,"@context":73},[15,34,56],{"@type":16,"itemListElement":17},"BreadcrumbList",[18,23,27,31],{"item":19,"name":20,"@type":21,"position":22},"https://docshare.wps.com","Home","ListItem",1,{"item":24,"name":25,"@type":21,"position":26},"https://docshare.wps.com/document/","Document",2,{"item":28,"name":29,"@type":21,"position":30},"https://docshare.wps.com/document/research-report/","Research & Report",3,{"item":32,"name":10,"@type":21,"position":33},"https://docshare.wps.com/document/characterization-of-m6a-methylation-modifications-in-gastric-cancer/333080/",4,{"url":32,"name":10,"@type":35,"image":36,"author":41,"headline":10,"publisher":44,"fileFormat":47,"inLanguage":8,"description":12,"dateModified":48,"datePublished":49,"encodingFormat":47,"isAccessibleForFree":50,"interactionStatistic":51},"DigitalDocument",{"url":37,"@type":38,"width":39,"height":40},"https://docshare.wps.com/thumbnails/characterization-of-m6a-methylation-modifications-in-gastric-cancer/333080.png","ImageObject",300,407,{"name":42,"@type":43},"Logic","Person",{"url":19,"name":45,"@type":46},"DocShare","Organization","application/pdf","2026-10-23","2026-09-21",true,{"@type":52,"interactionType":53,"userInteractionCount":55},"InteractionCounter",{"@type":54},"ViewAction",6,{"@type":57,"mainEntity":58},"FAQPage",[59,65,69],{"name":60,"@type":61,"acceptedAnswer":62},"What is the focus of the study on m6A in gastric cancer?","Question",{"text":63,"@type":64},"The study investigates potential roles of m6A RNA modifications in gastric cancer and how m6A patterns relate to tumor biology and treatment response.","Answer",{"name":66,"@type":61,"acceptedAnswer":67},"How were m6A modification patterns and the m6Ascore determined?",{"text":68,"@type":64},"m6A patterns were evaluated using 18 m6A regulators, and the m6Ascore was constructed using principal component analysis.",{"name":70,"@type":61,"acceptedAnswer":71},"What key findings link m6A patterns with prognosis and immunotherapy response?",{"text":72,"@type":64},"Three m6A modification patterns were identified, including one associated with immune activation and better survival. The study also reports that the m6Ascore serves as an independent prognostic biomarker and may help predict responses to immune checkpoint blockade therapy.","https://schema.org",{"og:url":32,"og:type":75,"og:title":10,"og:site_name":45,"og:description":12},"article",{"robots":77,"canonical":32},"index,follow",{"doc_id":79,"site_id":7},333080,1790159688,{"code":4,"msg":82,"data":83},"success",{"doc_id":79,"user_id":84,"nickname":42,"user_avatar":85,"doc_module":4,"category_id":86,"category_name":29,"doc_title":10,"doc_description":12,"doc_content":87,"file_id":88,"file_url":89,"file_type":90,"file_size":91,"view_count":55,"is_deleted":4,"is_public":22,"is_downloadable":22,"audit_status":22,"page_count":92,"language":93,"language_code":8,"site_id":7,"html_lang":8,"table_of_contents":94,"faqs":95,"seo_title":96,"seo_description":12,"update_tm":97,"read_time":98,"preview_page_count":4},1099513958762,"https://ap-avatar.wpscdn.com/avatar/1000023916a998db790?x-image-process=image/resize,m_fixed,w_180,h_180&k=1784791008015729253",8,"[www.aging-us.com](www.aging-us.com) AGING 2024, Vol. 16, No. 1  \nResearch Paper  \nCharacterization of m6A methylation modifications in gastric cancer  \nWei Yin1,*, Zhanwei Huo2,*, Jiawei Zuo3,*, Haixiao Wang4, Bi Chen5, Liqing Zhou3  \n1 Department of Gastrointestinal Surgery, The Affiliated Huai’an Hospital of Xuzhou Medical University and The Second People’s Hospital of Huai’an, Huai’an 223300, Jiangsu, China  \n2 Department of General Surgery, Lianshui People’s Hospital Affiliated to Kangda College of Nanjing Medical University, Huai’an 223300, Jiangsu, China  \n3 Department of Radiotherapy, The Affiliated Huai’an Hospital of Xuzhou Medical University and The Second People’s Hospital of Huai’an, Huai’an 223300, Jiangsu, China  \n4 Department of General Surgery, The Affiliated Huai’an No. 1 People’s Hospital of Nanjing Medical University, Huai’an 223300, Jiangsu, China  \n5 Department of Rehabilitation, Geriatric Hospital of Nanjing Medical University, Jiangsu Province Official Hospital, Nanjing 210000, Jiangsu, China  \n* Equal contribution  \nCorrespondence to: Wei Yin, Liqing Zhou; email: [xzmuyinwei@njucm.edu.cn](xzmuyinwei@njucm.edu.cn), [zhouliqing@hasdermyy.wecom.work](zhouliqing@hasdermyy.wecom.work)[ ](zhouliqing@hasdermyy.wecom.work)[Keywords:](Keywords: m6A)[ m6A](Keywords: m6A), tumor microenvironment, immunotherapy, gastric cancer  \nReceived: August 6, 2023 Accepted: November 6, 2023 Published: January 10, 2024  \nCopyright: © 2024 Yin et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.  \nABSTRACT  \nWidely recognized as an essential epitranscriptomic modification, RNA N6-methyladenosine (m6A) is involved in both physiological and pathological processes. Here, we want to investigate m6A modification’s potential roles in gastric cancer. Gastric cancer samples were selected from TCGA-STAD and GEO (GSE84426, GSE84433) datasets. Based on 18 regulators of m6A, m6A modification patterns were thoroughly evaluated in gastric cancer samples. Principal component analysis algorithms were used to construct the m6Ascore, using which, m6A modification features in tumor somatic mutations and immune checkpoint blockade therapy were analyzed. 34 gastric cancer samples were collected to verify the effectiveness of the m6Ascore. Here, we determined three different m6A modification patterns. m6Acluster-C modification pattern presented immune activation-associated enrichment pathways and have significant survival advantages. Then, in gastric cancer, m6Ascore could act as an independent prognostic biomarker. A significant survival benefit was exhibited inpatients with high m6Ascore. Moreover, the modification signature of m6A uncovered in this study would help to predict immune checkpoint blockade therapy’s responses. In conclusion, our discoveries all pointed to the fact that modification patterns of m6A were linked to the TME. Moreover, evaluation of individual tumor’s m6A modification pattern will help to guide immunotherapy strategies that shows more therapeutic effects.  \nINTRODUCTION  \nGastric cancer, the fifth most common cancer in the world, ranked fourth in the most common causes of cancer-related death. In 2020, 1,089,103 new incidence of gastric cancer and 768,793 deaths related to gastric cancer were recorded (equating to one in every 13 deaths globally) [1, 2] . In male, the incidence rate is 2-fold  \nhigher than that in female. The American Cancer Society’s estimates for gastric cancer in the United States for 2023 are that about 26,500 new cases and about 11,130 deaths from this type of cancer [3] . Recently, it is found that in younger generations, especially those below 50-year-old, the incidence rates of gastric cancer have increased [4] . In all malignant tumors of the stomach, adenocarcinoma, the most common histologic  \ns","cbCaimIhJlXK9A0V","https://ap.wps.com/l/cbCaimIhJlXK9A0V","pdf",3953425,17,"English","# Abstract\n# Introduction","[{\"question\":\"What is the focus of the study on m6A in gastric cancer?\",\"answer\":\"The study investigates potential roles of m6A RNA modifications in gastric cancer and how m6A patterns relate to tumor biology and treatment response.\"},{\"question\":\"How were m6A modification patterns and the m6Ascore determined?\",\"answer\":\"m6A patterns were evaluated using 18 m6A regulators, and the m6Ascore was constructed using principal component analysis.\"},{\"question\":\"What key findings link m6A patterns with prognosis and immunotherapy response?\",\"answer\":\"Three m6A modification patterns were identified, including one associated with immune activation and better survival. The study also reports that the m6Ascore serves as an independent prognostic biomarker and may help predict responses to immune checkpoint blockade therapy.\"}]","Characterization of m6A methylation modifications in gastric cancer | PDF",1790000769,43,{"code":4,"msg":82,"data":100},[101,105,109,113,118,122,127,130,135,138,142],{"id":22,"doc_module":4,"doc_module_name":25,"category_name":102,"show_sort_weight":103,"slug":104},"Story & Novel",90,"story-novel",{"id":26,"doc_module":4,"doc_module_name":25,"category_name":106,"show_sort_weight":107,"slug":108},"Literature",80,"literature",{"id":33,"doc_module":4,"doc_module_name":25,"category_name":110,"show_sort_weight":111,"slug":112},"Exam",70,"exam",{"id":114,"doc_module":4,"doc_module_name":25,"category_name":115,"show_sort_weight":116,"slug":117},5,"Comic",60,"comic",{"id":55,"doc_module":4,"doc_module_name":25,"category_name":119,"show_sort_weight":120,"slug":121},"Technology",50,"technology",{"id":123,"doc_module":4,"doc_module_name":25,"category_name":124,"show_sort_weight":125,"slug":126},7,"Healthcare",40,"healthcare",{"id":86,"doc_module":4,"doc_module_name":25,"category_name":29,"show_sort_weight":128,"slug":129},30,"research-report",{"id":131,"doc_module":4,"doc_module_name":25,"category_name":132,"show_sort_weight":133,"slug":134},9,"Religion & Spirituality",20,"religion-spirituality",{"id":133,"doc_module":4,"doc_module_name":25,"category_name":136,"show_sort_weight":133,"slug":137},"World Cup","world-cup",{"id":139,"doc_module":4,"doc_module_name":25,"category_name":140,"show_sort_weight":139,"slug":141},10,"Lifestyle","lifestyle",{"id":143,"doc_module":4,"doc_module_name":25,"category_name":144,"show_sort_weight":114,"slug":145},19,"General","general"]