[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-327709-105":59,"doc-detail-327709-en":131},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":124,"head_meta":126,"extra_data":128,"updated_unix":130},105,"en","cervical-extracellular-matrix-hydrogel-optimizes-tumor-heterogeneity-of-cervical-squamous-cell-carcinoma-organoids-research-study-summary","Cervical extracellular matrix hydrogel optimizes tumor heterogeneity of cervical squamous cell carcinoma organoids - Research study summary","","Cervical cancer, especially cervical squamous cell carcinoma, remains difficult to model because common organoid culture relies on Matrigel, which does not reproduce the tissue-specific cervical microenvironment and can distort tumor heterogeneity. Researchers collected patient-derived paracancerous cervical tissues and prepared uterine cervix extracellular matrix (UCEM) hydrogels. Proteomics and RNA sequencing show UCEM-organoids better match native CSCC, preserve relevant signaling pathways, and display tumorigenic potential, including chemotherapy resistance, offering improved organoid modeling using native matrix hydrogels.",{"@graph":69,"@context":123},[70,84,106],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/cervical-extracellular-matrix-hydrogel-optimizes-tumor-heterogeneity-of-cervical-squamous-cell-carcinoma-organoids-research-study-summary/327709/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/cervical-extracellular-matrix-hydrogel-optimizes-tumor-heterogeneity-of-cervical-squamous-cell-carcinoma-organoids-research-study-summary/327709.png","ImageObject",300,407,{"name":92,"@type":93},"Eliana","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-23","2026-09-21",true,{"@type":102,"interactionType":103,"userInteractionCount":105},"InteractionCounter",{"@type":104},"ViewAction",16,{"@type":107,"mainEntity":108},"FAQPage",[109,115,119],{"name":110,"@type":111,"acceptedAnswer":112},"Why is Matrigel inadequate for modeling cervical squamous cell carcinoma organoids?","Question",{"text":113,"@type":114},"Matrigel does not accurately replicate the tissue-specific cervical microenvironment and shows biochemical/species differences. It can therefore fail to represent tumor heterogeneity and individual differences accurately.","Answer",{"name":116,"@type":111,"acceptedAnswer":117},"How were UCEM hydrogels prepared and what do they support in organoid culture?",{"text":118,"@type":114},"Paracancerous cervical tissues were collected from patients with cervical squamous cell carcinoma, then used to prepare uterine cervix extracellular matrix (UCEM) hydrogels. UCEM provides a tailored microenvironment that supports CSCC organoid formation, growth, and maintained tumorigenic potential.",{"name":120,"@type":111,"acceptedAnswer":121},"What evidence indicates UCEM-organoids reflect native tumor heterogeneity?",{"text":122,"@type":114},"Proteomic analysis identified tissue-specific signaling components and maintained cervical proteins in UCEM. RNA sequencing showed UCEM-organoids exhibited greater similarity to native CSCC and activation of CSCC-associated pathways reflecting tumor heterogeneity.","https://schema.org",{"og:url":83,"og:type":125,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":127,"canonical":83},"index,follow",{"doc_id":129,"site_id":62},327709,1790382691,{"code":4,"msg":5,"data":132},{"doc_id":129,"user_id":133,"nickname":92,"user_avatar":134,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":135,"file_id":136,"file_url":137,"file_type":138,"file_size":139,"view_count":105,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":140,"language":141,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":142,"faqs":143,"seo_title":144,"seo_description":67,"update_tm":145,"read_time":146,"preview_page_count":4},4398048949847,"https://ap-avatar.wpscdn.com/avatar/400002536579ef2da7f?_k=1778318612642679267","CANCER  \nCervical extracellular matrix hydrogel optimizes tumor heterogeneity of cervical squamous cell  \ncarcinoma organoids  \nHaonan Song1†, Haoyuan Jiang1†, Weichu Hu1†, Yan Hai1, Yihuan Cai1, Hu Li2, Yuru Liao1, Yi Huang3, Xiaogang Lv4, Yefei Zhang1, Jiping Zhang5, Yan Huang6, Xiaomei Liang1, Hao Huang3*, Xinhua Lin7,8*, Yifeng Wang1*, Xiao Yi1,7*  \nCervical cancer, primarily squamous cell carcinoma, is the most prevalent gynecologic malignancy. Organoids can mimic tumor development in vitro, but current Matrigel inaccurately replicates the tissue-specific microenvironment. This limitation compromises the accurate representation of tumor heterogeneity. We collected paracancerous cervical tissues from patients diagnosed with cervical squamous cell carcinoma (CSCC) and prepared uterine cervix extracellular matrix (UCEM) hydrogels. Proteomic analysis of UCEM identified several tissue-specific signaling pathways including human papillomavirus, phosphatidylinositol 3-kinase–AKT, and extracellular matrix receptor. Secreted proteins like FLNA, MYH9, HSPA8, and EEF1A1 were present, indicating UCEM successfully maintained cervical proteins. UCEM provided a tailored microenvironment for CSCC organoids, enabling formation and growth while preserving tumorigenic potential. RNA sequencing showed UCEM-organoids exhibited greater similarity to native CSCC and reflected tumor heterogeneity by exhibiting CSCC-associated signaling pathways including virus protein-cytokine, nuclear factor κB, tumor necrosis factor, and oncogenes EGR1, FPR1, and IFI6. Moreover, UCEM-organoids developed chemotherapy resistance. Our research provides insights into advanced organoid technology through native matrix hydrogels.  \ncopyright © 2024 the Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. no claim to original U.S.  \nGovernment Works. distributed under a creative commons Attribution noncommercial license 4.0 (cc BY-nc) .  \nINTRODUCTION  \nCervical cancer is the most common gynecological malignancy anda leading cause of cancer-related death in women, particularly in countries with low development indices ( 1, 2) . Although human papillomavirus (HPV) has been identified as the primary cause of cervical cancer, and worldwide efforts are being made to eradicate the disease through HPV vaccination and early screening, a notable number of patients are still being diagnosed each year. Cervical squamous cell carcinoma (CSCC) is the predominant tissue type of cervical cancer, accounting for more than 70%(3) . Surgery, radiotherapy, and comprehensive chemotherapy offer limited success in curing patients and often come with significant side effects and risk of complications, making precise medical treatment elusive. Cancer cell lines and tumor tissue xenotransplantation models commonly used in research fail to accurately replicate the native pathological state of human CSCC in vitro due to the absence of the specific microenvironment of human cervical tissue. Therefore, reproducing tumor occurrence in vitro remains challenging (4, 5) . Consequently, these  \n1department of Gynecology, Zhujiang hospital, Southern Medical University, Guangzhou 510280, china. 2the First Affiliated hospital, Jinan University, Guangzhou 510280, china. 3department of Gynecology, the Sixth Affiliated hospital, South china University of technology, Foshan 528200, china. 4department of Gynecologic Oncology, Affiliated cancer hospital and institute of Guangzhou Medical University, Guangzhou 510030, china. 5department of Gynecology, Affiliated Foshan Maternity & child healthcare hospital, Southern Medical University, Foshan, china. 6Second department of hepatobiliary Surgery, Zhujiang hospital, Southern Medical University, Guangzhou 510280, china. 7Greater Bay Area institute of Precision Medicine, Guangzhou 510280, china. 8State Key laboratory of Genetic engineering, School of life Sciences, Zhongshan hospital, Fudan University Shanghai, Shan","cbCainS7Ke6xgni3","https://ap.wps.com/l/cbCainS7Ke6xgni3","pdf",7159339,18,"English","# Introduction\n## Limitations of current organoid models (Matrigel)\n## Need for tissue-specific microenvironments\n# Study approach overview\n## UCEM hydrogel preparation from patient tissues\n## Proteomics and RNA sequencing to assess similarity\n## Functional outcomes: growth, tumor heterogeneity, and therapy response","[{\"question\":\"Why is Matrigel inadequate for modeling cervical squamous cell carcinoma organoids?\",\"answer\":\"Matrigel does not accurately replicate the tissue-specific cervical microenvironment and shows biochemical/species differences. It can therefore fail to represent tumor heterogeneity and individual differences accurately.\"},{\"question\":\"How were UCEM hydrogels prepared and what do they support in organoid culture?\",\"answer\":\"Paracancerous cervical tissues were collected from patients with cervical squamous cell carcinoma, then used to prepare uterine cervix extracellular matrix (UCEM) hydrogels. UCEM provides a tailored microenvironment that supports CSCC organoid formation, growth, and maintained tumorigenic potential.\"},{\"question\":\"What evidence indicates UCEM-organoids reflect native tumor heterogeneity?\",\"answer\":\"Proteomic analysis identified tissue-specific signaling components and maintained cervical proteins in UCEM. RNA sequencing showed UCEM-organoids exhibited greater similarity to native CSCC and activation of CSCC-associated pathways reflecting tumor heterogeneity.\"}]","Cervical extracellular matrix hydrogel optimizes tumor heterogeneity of cervical squamous cell carcinoma organoids - Research study summary | PDF",1789977871,45]