[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-367665-105":59,"doc-detail-367665-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","cerna-network-reveals-potential-diagnostic-biomarkers-or-immunotherapy-targets-for-hypopharyngeal-squamous-cell-carcinoma","CeRNA network reveals potential diagnostic biomarkers or immunotherapy targets for Hypopharyngeal squamous cell carcinoma","","Hypopharyngeal squamous cell carcinoma (HSCC) lacks early detection options, and improved diagnostic and prognostic markers are urgently needed. This study constructs a ceRNA network by identifying differentially expressed circular RNAs and mRNAs between HSCC and normal controls, then uses univariate Cox analysis, survival curves, and diagnostic evaluation. Bioinformatic integration links candidate biomarkers to the tumor microenvironment and immunotherapy response, with qRT-PCR validating expression in clinical and GSE2379 samples.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/cerna-network-reveals-potential-diagnostic-biomarkers-or-immunotherapy-targets-for-hypopharyngeal-squamous-cell-carcinoma/367665/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/cerna-network-reveals-potential-diagnostic-biomarkers-or-immunotherapy-targets-for-hypopharyngeal-squamous-cell-carcinoma/367665.png","ImageObject",300,407,{"name":92,"@type":93},"Cart","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-11-01","2026-09-23",true,{"@type":102,"interactionType":103,"userInteractionCount":44},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What is the main objective of the study on HSCC?","Question",{"text":112,"@type":113},"To construct a ceRNA network and identify potential diagnostic biomarkers for hypopharyngeal squamous cell carcinoma (HSCC), while also assessing links to prognosis and immunotherapy relevance.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How were candidate biomarkers selected?",{"text":117,"@type":113},"Differentially expressed circRNAs and mRNAs between HSCC and normal controls were identified using R. Univariate Cox analysis and survival curves were applied to find biomarkers associated with HSCC prognosis, followed by diagnostic evaluation.",{"name":119,"@type":110,"acceptedAnswer":120},"Which biomarkers were identified and how were they validated?",{"text":121,"@type":113},"Three genes—NRG1, CCNG2, and CHSY1—were identified as prognostic and diagnostic biomarkers. Their expression patterns were validated by qRT-PCR in clinical HSCC and para-cancerous tissues, consistent with observations from the GSE2379 dataset.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},367665,1790809646,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":44,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":52,"language":139,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":140,"faqs":141,"seo_title":142,"seo_description":67,"update_tm":143,"read_time":144},18829141979164,"https://eur-avatar.wpscdn.com/davatar_6f874abed73319feea01a86fa6f0fab8","Brazilian Journal of Otorhinolaryngology 91 (2025) 101655  \nContents lists available at ScienceDirect  \nBrazilian Journal of Otorhinolaryngology  \njournal [homepage:](homepage: www.bjorl.org)[ www.bjorl.org](homepage: www.bjorl.org)  \n| Original article\u003Cbr>CeRNA network reveals potential diagnostic biomarkers or immunotherapy targets for Hypopharyngeal squamous cell carcinoma |  | |\n| --- | --- | --- |\n| Xi Yanga, Chun Feng b, Donghui Jiang a, Xin Xu a , Yingying Zhang c, Jin Wang d,\u003Cbr>Xiaoguang He a,*\u003Cbr>a The first affiliated Hospital of Kunming Medical University, The Second Department of Otolaryngology, Head and Neck Surgery, Kunming, Yunnan, China\u003Cbr>b The Affiliated Hospital of Kunming University of Science and Technology, The First People’s Hospital of Yunnan Province, Department of Otolaryngology, Kunming, China\u003Cbr>c Kunming Medical University, School of Basic Medical Science, Department of Pathology and Pathophysiology, Kunming, Yunnan, China\u003Cbr>d The Affiliated Hospital of Yunnan University, The Second People’s Hospital of Yunnan Province, Department of Otolaryngology, Kunming, China |  |  |\n| A R T I C L E I N F O\u003Cbr>Edited by Dr Carlos Takahiro Chone.\u003Cbr>Keywords:\u003Cbr>Hypopharyngeal squamous cell carcinoma circRNA\u003Cbr>ceRNA network Biomarker | A B S T R A C T\u003Cbr>Objective: This study aimed to construct a ceRNA network and identify potential diagnostic biomarkers for HSCC. Methods: The R software was used to identify Differentially Expressed circular RNAs (DE-circRNAs) and mRNAs (DE-mRNAs) between HSCC and Normal Control (NC) specimens. Univariate Cox analysis and survival curves were used to identify biomarkers associated with the prognosis of HSCC. In addition, the diagnostic value of the biomarkers and their relevance to the tumor microenvironment and immunotherapy were evaluated. A ceRNA network based on mRNAs, circRNAs, and miRNAs was established. Finally, qRT-PCR was used to evaluate the expression of biomarkers in HSCC and para-cancerous tissue samples.\u003Cbr>Results: A network containing 90 miRNAs, 47 circRNAs, and 111 mRNAs was established via bioinformatic analysis. Three genes (NRG1, CCNG2, and CHSY1) were identified as prognostic and diagnostic biomarkers for HSCC. The low-CCNG2-expression and low-CHSY1-expression groups had higher survival rates, whereas the lowNRG1-expression group had a lower survival rate. The AUC values of the three biomarkers > 0.9, which indicated that the diagnostic value of all biomarkers was excellent. Moreover, CCNG2 and CHSY1 were associated with the severity of malignancy and the effectiveness of immunotherapy. Subsequently, a ceRNA network containing 3 mRNAs, 7 miRNAs, and 7 circRNAs was established. The results of qRT-PCR validated that the expression patterns of NRG1 and CHSY1 in clinical HSCC and para-cancerous samples were consistent with those observed in the GSE2379 dataset.\u003Cbr>Conclusion: In this study, we identified three diagnostic biomarkers for HSCC (NRG1, CCNG2, and CHSY1) and established their corresponding ceRNA network via bioinformatic analysis, providing novel insights into the screening and treatment of HSCC. |  |\n\nIntroduction  \nHypopharyngeal Squamous Cell Carcinoma (HSCC) is an aggressive tumour originating from the outer layer (epithelium) of the upper aerodigestive tract1 and accounts for 5%–15% of all Head and Neck Squamous Cell Carcinoma (HNSCC).2 Due to the unique anatomical  \nlocation, it is undetectable in the early stages. At present, HSCC is treated with surgical resection combined with adjuvant chemotherapy and radiotherapy in clinical, with a 5-year survival rate of approximately 35%.3,4 Therefore, it is critical to identify novel diagnostic and prognostic biomarkers for HSCC and understand the pathological mechanisms underlying its development.  \nXi Yang: 0000-0002-9476-0056  \nChun Feng: 0000-0002-5086-0573  \nDongHui Jiang: 0000-0002-8590-2454  \nYingying Zhang: 0009-0001-3744-8779  \nJin Wang: 0009-0005-5796-7612  \n* Corresponding author.  \nE-mai","cbCaiikvSFiL7ew0","https://ap.wps.com/l/cbCaiikvSFiL7ew0","pdf",8136796,"English","# Introduction\n# Materials and Methods\n# Results\n# Conclusion\n# Keywords","[{\"question\":\"What is the main objective of the study on HSCC?\",\"answer\":\"To construct a ceRNA network and identify potential diagnostic biomarkers for hypopharyngeal squamous cell carcinoma (HSCC), while also assessing links to prognosis and immunotherapy relevance.\"},{\"question\":\"How were candidate biomarkers selected?\",\"answer\":\"Differentially expressed circRNAs and mRNAs between HSCC and normal controls were identified using R. Univariate Cox analysis and survival curves were applied to find biomarkers associated with HSCC prognosis, followed by diagnostic evaluation.\"},{\"question\":\"Which biomarkers were identified and how were they validated?\",\"answer\":\"Three genes—NRG1, CCNG2, and CHSY1—were identified as prognostic and diagnostic biomarkers. Their expression patterns were validated by qRT-PCR in clinical HSCC and para-cancerous tissues, consistent with observations from the GSE2379 dataset.\"}]","CeRNA network reveals potential diagnostic biomarkers or immunotherapy targets for Hypopharyngeal squamous cell carcinoma | PDF",1790171872,25]