[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-383454-105":59,"doc-detail-383454-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","cell-surface-vimentin-detection-in-cancer-cells-by-peptide-based-monoclonal-antibody","Cell Surface Vimentin Detection in Cancer Cells by Peptide-Based Monoclonal Antibody","","Vimentin is an intermediate filament protein expressed across mesenchymal cell types, and its dysregulation and abnormal cell-surface localization are linked to malignant behavior. This study generates and characterizes anti-vimentin monoclonal antibodies using a 14-mer vimentin-derived peptide conjugated to KLH for immunization and conventional hybridoma production. Purification via affinity chromatography and validation with ELISA, IP-Western blotting, immunocytochemistry, and flow cytometry identify clone 7C11-D9 as highly reactive. The antibody detects vimentin in normal and cancerous cells while no vimentin expression is observed in healthy PBMC.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/cell-surface-vimentin-detection-in-cancer-cells-by-peptide-based-monoclonal-antibody/383454/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/cell-surface-vimentin-detection-in-cancer-cells-by-peptide-based-monoclonal-antibody/383454.png","ImageObject",300,407,{"name":92,"@type":93},"Ivy","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-11-01","2026-09-24",true,{"@type":102,"interactionType":103,"userInteractionCount":39},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What is the main goal of this study?","Question",{"text":112,"@type":113},"To generate and characterize anti-vimentin monoclonal antibodies based on a synthetic vimentin-derived peptide, aimed at detecting or targeting cell-surface vimentin in cancer contexts.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How was the peptide-based immunogen prepared?",{"text":117,"@type":113},"A 14-mer peptide from human vimentin was selected, modified with an added cysteine for conjugation, and conjugated separately to carrier proteins KLH and BSA under described identical conditions.",{"name":119,"@type":110,"acceptedAnswer":120},"Which antibody clone was selected and why?",{"text":121,"@type":113},"Among the produced hybridomas, the 7C11-D9 clone (IgG1, kappa) showed higher reactivity with the immunizing peptide and was selected for purification and characterization.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},383454,1790765978,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":39,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":39,"language":139,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":140,"faqs":141,"seo_title":142,"seo_description":67,"update_tm":143,"read_time":46},549758252649,"https://ap-avatar.wpscdn.com/avatar/8000253669c5317157?_k=1778319167496531819","Cell Surface Vimentin Detection in Cancer Cells by Peptide-Based Monoclonal Antibody  \nNiloufar Sadeghi 1, Ghazaleh Fazli 1, Ali Ahmad Bayat 1, Raminasadat Fatemi 1, Nasim Ebrahimnejhad 1,  \nAli Salimi 1, Omid Zarei 2*, and Hodjattallah Rabbani 1*  \n1. Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran  \n2. Cellular and Molecular Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran  \n* Corresponding authors: Omid Zarei, Ph.D., Cellular and Molecular Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran  \nHodjattallah Rabbani, Ph.D., Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran, Tel: +98 87 33664643,  \n21 22432020  \nFax: +98 21 22432021  \nE-mail:  \n[omidzarei58@gmail.com](omidzarei58@gmail.com); [o.zarei@muk.ac.ir](o.zarei@muk.ac.ir), [hodrab@gmail.com](hodrab@gmail.com); [rabbani@ari.ir](rabbani@ari.ir)[ ](rabbani@ari.ir)Received: 22 Aug 2022  \nAccepted: 7 Jan 2023  \nAbstract  \nBackground: Vimentin is a prominent Intermediate Filaments (IFs) protein expressed in different mesenchymal origin cell types. Besides a wide range of cellular function roles associated with vimentin expression, its dysregulation and cell surface expression in the induction of malignancy properties have been reported extensively, making it a promising cancer-specific target. Therefore, this study aimed to generate and characterize anti-vimentin monoclonal antibodies.  \nMethods: A 14-mer synthetic peptide from vimentin was conjugated to Keyhole Limpet Hemocyanin (KLH) and used for immunization of Blab/C mice and monoclonal production by conventional hybridoma technology. The monoclonal antibody was purified using affinity chromatography of supernatants from the selected hybridoma cells. ELISA, Immunoprecipitation-Western blotting (IP-WB), Immunocytochemistry (ICC), and flow cytometry were employed to characterize the produced monoclonal antibody in terms of interaction with vimentin immunizing peptide as well as vimentin protein. Results: Amid the several obtained producing anti-vimentin antibody hybridomas, the 7C11-D9 clone (IgG1 isotype with kappa light chain) showed higher reactivity with the immunizing peptide, and led to its selection for purification and characterization. The purified antibody could detect vimentin protein in IP-WB, ICC and flow cytometry of the normal and cancerous cells with different origin. No vimentin expression was found in normal healthy Peripheral Blood Mononuclear Cell (PBMC) .  \nConclusion: Taken together, 7C11-D9 anti-vimentin monoclonal antibody might be used as immune diagnostic or immune therapeutic tool where detection or targeting of vimentin in a wide range of organisms is required.  \nKeywords: Antibody, Cancer, Peptide, Targeted therapy, Vimentin  \nTo cite this article: Sadeghi N, Fazli Gh, Bayat AA, Fatemi R, Ebrahimnejhad N, Salimi A, et al. Cell Surface Vimentin Detection in Cancer Cells by Peptide-Based Monoclonal Antibody. Avicenna J Med Biotech 2023;15(2):68-75.  \nIntroduction  \nIntermediate Filaments (IFs) - in combination with microfilaments and microtubules- are constructive for the filamentous cytoskeleton system 1. IFs containing different proteins with shared properties in sequence/ structure point of view are classified into six types, nominated as type I to type VI 2. Vimentin is a significant type III protein of IFs composed of 466 amino acids (~53 kDa molecular weight) . It is expressed indifferent mesenchymal origin cell types with expression patterns from nucleus to cell surface 3,4 . Although, vimentin is known as a cytoskeleton protein responsible for the maintenance of cell structural integrity, a wide range of cellular functions such as cell adhesion, motility, migration, survival, and wound healing have  \nbeen associated with vimentin expression and its interaction with other proteins 5-8.  ","cbCaikC6lgm1hZSW","https://ap.wps.com/l/cbCaikC6lgm1hZSW","pdf",514665,"English","# Abstract\n# Introduction\n# Materials and Methods","[{\"question\":\"What is the main goal of this study?\",\"answer\":\"To generate and characterize anti-vimentin monoclonal antibodies based on a synthetic vimentin-derived peptide, aimed at detecting or targeting cell-surface vimentin in cancer contexts.\"},{\"question\":\"How was the peptide-based immunogen prepared?\",\"answer\":\"A 14-mer peptide from human vimentin was selected, modified with an added cysteine for conjugation, and conjugated separately to carrier proteins KLH and BSA under described identical conditions.\"},{\"question\":\"Which antibody clone was selected and why?\",\"answer\":\"Among the produced hybridomas, the 7C11-D9 clone (IgG1, kappa) showed higher reactivity with the immunizing peptide and was selected for purification and characterization.\"}]","Cell Surface Vimentin Detection in Cancer Cells by Peptide-Based Monoclonal Antibody | PDF",1790257129]