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The document provides features, usage guidance, feed ratio, stability, specifications, safety precautions for Cell Boost 7b, storage conditions, and quality control test parameters.",{"@graph":14,"@context":73},[15,34,56],{"@type":16,"itemListElement":17},"BreadcrumbList",[18,23,27,31],{"item":19,"name":20,"@type":21,"position":22},"https://docshare.wps.com","Home","ListItem",1,{"item":24,"name":25,"@type":21,"position":26},"https://docshare.wps.com/document/","Document",2,{"item":28,"name":29,"@type":21,"position":30},"https://docshare.wps.com/document/healthcare/","Healthcare",3,{"item":32,"name":10,"@type":21,"position":33},"https://docshare.wps.com/document/cell-boost-7a-and-7b-supplements-technical-product-information/400830/",4,{"url":32,"name":10,"@type":35,"image":36,"author":41,"headline":10,"publisher":44,"fileFormat":47,"inLanguage":8,"description":12,"dateModified":48,"datePublished":49,"encodingFormat":47,"isAccessibleForFree":50,"interactionStatistic":51},"DigitalDocument",{"url":37,"@type":38,"width":39,"height":40},"https://docshare.wps.com/thumbnails/cell-boost-7a-and-7b-supplements-technical-product-information/400830.png","ImageObject",300,407,{"name":42,"@type":43},"Aria","Person",{"url":19,"name":45,"@type":46},"DocShare","Organization","application/pdf","2026-10-30","2026-09-27",true,{"@type":52,"interactionType":53,"userInteractionCount":55},"InteractionCounter",{"@type":54},"ViewAction",9,{"@type":57,"mainEntity":58},"FAQPage",[59,65,69],{"name":60,"@type":61,"acceptedAnswer":62},"What are HyClone Cell Boost 7a and 7b supplements intended for?","Question",{"text":63,"@type":64},"They are intended for use with HyClone ActiPro and ActiSM media as part of the ActiPro cell culture system to support fed-batch production of recombinant proteins using CHO cell lines.","Answer",{"name":66,"@type":61,"acceptedAnswer":67},"What are the key formulation characteristics of Cell Boost 7a and 7b?",{"text":68,"@type":64},"Both are chemically defined, animal-derived component-free (ADCF) supplements optimized for high-yield protein production, and they do not contain growth factors, peptides, hydrolysates, phenol red, or 2-mercaptoethanol.",{"name":70,"@type":61,"acceptedAnswer":71},"How should Cell Boost 7a and 7b be added during preparation?",{"text":72,"@type":64},"They should be added to the cultivation vessel as individual solutions and not mixed in advance, because advance mixing can cause precipitation. 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They are available as a convenient ready-to-use liquid feed solution or as a dry powder concentrated format to be hydrated before use (Fig 1) . Cell Boost 7a and 7b are chemically defined (CD), animal-derived component-free (ADCF), and optimized for high-yield protein production in fed-batch processes. Cell Boost 7a and 7b do not contain any growth factors (such as insulin), peptides, hydrolysates, phenol red, or 2-mercaptoethanol, ensuring batch-to-batch consistency and increased cell culture process efficiency.  \nThe ActiPro system of media and supplements is designed with adaptation and production in mind. The ActiPro system is suitable for general biomanufacturing with CHO cell lines such as CHO-GS, CHO-K1, CHO-DG44, and CHO-S.  \nFeatures and benefits  \n• Demonstrated high yields of recombinant proteins (e.g., mAbs > 5 g/L) in fed-batch processes.  \n• Chemically defined ADCF formulations.  \n• Concentrated feeds that complement each other and are used together.  \n• Accompanied with detailed protocols for use to maximize output.  \nCell Boost supplements are used as an essential part of your cell culture feed strategy to enhance cell culture performance and increase product yield. Cell Boost 7a and 7b are intended for use together in defined concentrations.  \nThe supplements should be added to the cultivation vessel as individual solutions and should not be mixed in advance as this will cause precipitation. The recommended ratio of Cell Boost 7a to 7b is 10 to 1 (v/v) (example feeding strategy Cell Boost 7a at 3% and Cell Boost 7b at 0 . 3%) . The total amount of feed added and the specific feeding regime will need to be adjusted according to the nutritional requirements of each specific clone. The supplements are available as a powder or in a ready-to-use liquid format for research and process development. The liquid feed supplements meet testing specifications and are stable up to 12 months in PETE bottles and 9 months in bioprocessing bags at 2°C to 8°C (Fig 2) .  \nFig 1. ActiPro, ActiSM, and Cell Boost 7a and 7b are designed for high yields of recombinant proteins in fed-batch culture processes with CHO cells.  \n120  \n100  \n80  \n60  \n40  \n20  \n0  \n120  \n100  \n80  \n60  \n40  \n20  \n0  \nCell Boost 7a  Control  Lot 1  Lot 2  Lot 3  \nTime zero 3 months 6 months 9 months 12 months Cell Boost 7b  Control  Lot 1  Lot 2  Lot 3  \nTime zero 3 months 6 months 9 months 12 months  \nFig 2. Stability study testing of the Cell Boost 7a (A) and 7b (B) liquid feed supplements was performed using CHO-S cell line to determine viable cell density (VCD) compared with control (freshly hydrated powder). The study was conducted using three separate batches of Cell Boost 7a and 7b liquid feed supplements stored at 2°C to 8°C and used in fed-batch cultures of CHO-S cells 0, 3, 6, 9, and 12 months after preparation.  \n[cytiva.com](cytiva.com)  \nSpecifications  \nCell Boost 7a and 7b specifications for liquid feed solution and dry powder formats:  \n• Protein-free  \n• Growth factor-free  \n• Phenol red-free  \n• 2-mercaptoethanol-free  \n• Hydrolysate-free  \n• Chemically defined and ADCF formulated  \nCell Boost 7a  \nCell Boost 7a has a pH close to neutral and contains amino acids, vitamins, salts, trace elements, and glucose.  \nCell Boost 7b  \nCell Boost 7b has an alkaline pH and is a concentrated solution of amino acids.  \nSafety declaration  \nCell Boost 7b is a hazardous chemical:  \n• Causes skin irritation  \n• Causes severe eye irritation  \n• Can cause respiratory irritation  \nPlease take appropriate safety precautions prior to using Cell Boost 7b.  \n1. Wear protective gloves  \n2. Wear eye or face p","cbCaibw68DoFKMSt","https://ap.wps.com/l/cbCaibw68DoFKMSt","pdf",189555,"English","# Features and benefits\n## Intended use and system compatibility\n## Feeding strategy and handling\n## Stability and shelf-life\n## Specifications and quality control\n## Safety declaration\n## Storage","[{\"question\":\"What are HyClone Cell Boost 7a and 7b supplements intended for?\",\"answer\":\"They are intended for use with HyClone ActiPro and ActiSM media as part of the ActiPro cell culture system to support fed-batch production of recombinant proteins using CHO cell lines.\"},{\"question\":\"What are the key formulation characteristics of Cell Boost 7a and 7b?\",\"answer\":\"Both are chemically defined, animal-derived component-free (ADCF) supplements optimized for high-yield protein production, and they do not contain growth factors, peptides, hydrolysates, phenol red, or 2-mercaptoethanol.\"},{\"question\":\"How should Cell Boost 7a and 7b be added during preparation?\",\"answer\":\"They should be added to the cultivation vessel as individual solutions and not mixed in advance, because advance mixing can cause precipitation. The recommended ratio of 7a to 7b is 10 to 1 (v/v).\"}]","Cell Boost 7a and 7b Supplements - Technical Product Information | PDF",1790514667]