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PubMed searches using “carbohydrate sulfotransferase” plus defined criteria yielded 41 publications, and 40 CHST genetic/biochemical records were curated from UniProt, GeneCards, InterPro, and neXtProt. Findings link CHST loss to congenital connective tissue disorders and overexpression to multiple cancers and inflammatory states.",{"@graph":14,"@context":73},[15,34,56],{"@type":16,"itemListElement":17},"BreadcrumbList",[18,23,27,31],{"item":19,"name":20,"@type":21,"position":22},"https://docshare.wps.com","Home","ListItem",1,{"item":24,"name":25,"@type":21,"position":26},"https://docshare.wps.com/document/","Document",2,{"item":28,"name":29,"@type":21,"position":30},"https://docshare.wps.com/document/healthcare/","Healthcare",3,{"item":32,"name":10,"@type":21,"position":33},"https://docshare.wps.com/document/carbohydrate-sulfotransferases-a-review-of-emerging-diagnostic-and-prognostic-applications/396334/",4,{"url":32,"name":10,"@type":35,"image":36,"author":41,"headline":10,"publisher":44,"fileFormat":47,"inLanguage":8,"description":12,"dateModified":48,"datePublished":49,"encodingFormat":47,"isAccessibleForFree":50,"interactionStatistic":51},"DigitalDocument",{"url":37,"@type":38,"width":39,"height":40},"https://docshare.wps.com/thumbnails/carbohydrate-sulfotransferases-a-review-of-emerging-diagnostic-and-prognostic-applications/396334.png","ImageObject",300,407,{"name":42,"@type":43},"Ben ","Person",{"url":19,"name":45,"@type":46},"DocShare","Organization","application/pdf","2026-11-04","2026-09-26",true,{"@type":52,"interactionType":53,"userInteractionCount":55},"InteractionCounter",{"@type":54},"ViewAction",6,{"@type":57,"mainEntity":58},"FAQPage",[59,65,69],{"name":60,"@type":61,"acceptedAnswer":62},"What roles do carbohydrate sulfotransferases (CHST) play in cells?","Question",{"text":63,"@type":64},"CHST catalyze proteoglycan biosynthesis that enables interactions and signaling between neighboring cells. These processes occur in physiological and pathological states.","Answer",{"name":66,"@type":61,"acceptedAnswer":67},"How does CHST activity relate to congenital connective tissue disorders and cancers?",{"text":68,"@type":64},"A lack of CHST activity is associated with congenital connective tissue disorders. CHST overexpression has been detected in different malignancies.",{"name":70,"@type":61,"acceptedAnswer":71},"What evidence supports CHST as a diagnostic or prognostic marker, and what is needed next?",{"text":72,"@type":64},"The review summarizes reported diagnostic and prognostic applications, including gene mutations and unfavorable prognostic expression patterns. It also notes that larger clinical studies and robust analytical procedures are required for more reliable performance estimates.","https://schema.org",{"og:url":32,"og:type":75,"og:title":10,"og:site_name":45,"og:description":12},"article",{"robots":77,"canonical":32},"index,follow",{"doc_id":79,"site_id":7},396334,1792172851,{"code":4,"msg":82,"data":83},"success",[84,88,92,96,101,105,109,114,119,122,126],{"id":22,"doc_module":4,"doc_module_name":25,"category_name":85,"show_sort_weight":86,"slug":87},"Story & Novel",90,"story-novel",{"id":26,"doc_module":4,"doc_module_name":25,"category_name":89,"show_sort_weight":90,"slug":91},"Literature",80,"literature",{"id":33,"doc_module":4,"doc_module_name":25,"category_name":93,"show_sort_weight":94,"slug":95},"Exam",70,"exam",{"id":97,"doc_module":4,"doc_module_name":25,"category_name":98,"show_sort_weight":99,"slug":100},5,"Comic",60,"comic",{"id":55,"doc_module":4,"doc_module_name":25,"category_name":102,"show_sort_weight":103,"slug":104},"Technology",50,"technology",{"id":106,"doc_module":4,"doc_module_name":25,"category_name":29,"show_sort_weight":107,"slug":108},7,40,"healthcare",{"id":110,"doc_module":4,"doc_module_name":25,"category_name":111,"show_sort_weight":112,"slug":113},8,"Research & Report",30,"research-report",{"id":115,"doc_module":4,"doc_module_name":25,"category_name":116,"show_sort_weight":117,"slug":118},9,"Religion & Spirituality",20,"religion-spirituality",{"id":117,"doc_module":4,"doc_module_name":25,"category_name":120,"show_sort_weight":117,"slug":121},"World Cup","world-cup",{"id":123,"doc_module":4,"doc_module_name":25,"category_name":124,"show_sort_weight":123,"slug":125},10,"Lifestyle","lifestyle",{"id":127,"doc_module":4,"doc_module_name":25,"category_name":128,"show_sort_weight":97,"slug":129},19,"General","general",{"code":4,"msg":82,"data":131},{"doc_id":79,"user_id":132,"nickname":42,"user_avatar":133,"doc_module":4,"category_id":106,"category_name":29,"doc_title":10,"doc_description":12,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":55,"is_deleted":4,"is_public":22,"is_downloadable":22,"audit_status":22,"page_count":139,"language":140,"language_code":8,"site_id":7,"html_lang":8,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":12,"update_tm":144,"read_time":145,"preview_page_count":4},2336478951081,"https://ap-avatar.wpscdn.com/davatar_276721f389ce27ea32af1340a28f341c","Review  \nCarbohydrate sulfotransferases: a review of emerging diagnostic and prognostic applications  \nGramos Begolli1, Ivana Marković2,3, Jelena Knežević4,5, Željko Debeljak*2,3  \n1Clinic of medical biochemistry, University clinical center of Kosovo, Prishtina, Kosovo  \n2Clinical institute of laboratory diagnostics, University hospital centre Osijek, Osijek, Croatia  \n3Faculty of medicine, Josip Juraj Strossmayer University of Osijek, Osijek, Croatia  \n4Laboratory for advanced genomics, Ruđer Bošković Institute, Zagreb, Croatia  \n5Faculty for dental medicine and health, Josip Juraj Strossmayer University of Osijek, Osijek, Croatia  \n*[Corresponding author: zeljko.debeljak@gmail.com](Corresponding author: zeljko.debeljak@gmail.com)  \nAbstract  \nCarbohydrate sulfotransferases (CHST) catalyse the biosynthesis of proteoglycans that enable physical interactions and signalling between different neighbouring cells in physiological and pathological states. The study aim was to provide an overview of emerging diagnostic and prognostic applications of CHST. PubMed database search was conducted using the keywords “carbohydrate sulfotransferase” together with appropriate inclusion and exclusion criteria, whereby 41 publications were selected. Additionally, 40 records on CHST genetic and biochemical properties were hand-picked from UniProt, GeneCards, InterPro, and neXtProt databases.  \nCarbohydrate sulfotransferases have been applied mainly in diagnostics of connective tissue disorders, cancer and inflammations. The lack of CHST activity was found in congenital connective tissue disorders while CHST overexpression was detected in different malignancies. Mutations of CHST3 gene cause skeletal dysplasia, chondrodysplasia, and autosomal recessive multiple joint dislocations while increased tissue expression of CHST11, CHST12 and CHST15 is an unfavourable prognostic factor in ovarian cancer, glioblastoma and pancreatic cancer, respectively. Recently, CHST11 and CHST15 overexpression in the vascular smooth muscle cells was linked to the severe lung pathology in COVID-19 patients. Promising CHST diagnostic and prognostic applications have been described but larger clinical studies and robust analytical procedures are required for the more reliable diagnostic performance estimations.  \nKeywords: cancer; carbohydrate sulfotransferases; connective tissue; inflammation; tumour markers  \nSubmitted: March 7, 2023 Accepted: June 19, 2023  \nIntroduction  \nSulfation of small molecules, carbohydrates and proteins, is a set of metabolic reactions that occur in most organisms. This important modifying process takes place in bacteria, plants, and mammals. Sulfation is linked to a number of cellular signalling events and receptor-ligand binding modifications associated with physiological and pathological processes such as hormone regulation, cartilage creation, cancer cell spreading and solubilisation of harmful xenobiotics (1). In addition, sugar sulfation has also been shown to affect leukocyteendothelial cell adhesion at chronic inflammation  \nsites and expansion of neurons and astrocytes (2). Sulfations are catalysed by sulfotransferases (SULT), enzymes that are primarily responsible for the transfer of sulphate from a donor molecule, 3’-phosphoadenosine-5’-phosphosulfate (PAPS), to various hydroxyl and amine substrates (3). Two classes of SULT have been described: cytosolic SULT and membrane-associated SULT. Cytosolic SULT are involved in the sulfation of low-molecular-mass endogenous and exogenous compounds such as hormones, bioamines, drugs and other xenobiotic agents (4). On the other hand, glycosami-  \n[https://doi.org/10.11613/BM.2023.030503](https://doi.org/10.11613/BM.2023.030503) BiochemMed (Zagreb) 2023;33(3):030503  \n©Copyright by Croatian Society of Medical Biochemistry and Laboratory Medicine. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ([https://creative](https://creative)[commons.","cbCaidzhzudrQ7N5","https://ap.wps.com/l/cbCaidzhzudrQ7N5","pdf",179977,11,"English","# Abstract\n# Introduction\n## Background on sulfation and sulfotransferases\n## Roles of CHST and proteoglycans in cells and ECM\n# Clinical relevance of CHST in disease","[{\"question\":\"What roles do carbohydrate sulfotransferases (CHST) play in cells?\",\"answer\":\"CHST catalyze proteoglycan biosynthesis that enables interactions and signaling between neighboring cells. These processes occur in physiological and pathological states.\"},{\"question\":\"How does CHST activity relate to congenital connective tissue disorders and cancers?\",\"answer\":\"A lack of CHST activity is associated with congenital connective tissue disorders. CHST overexpression has been detected in different malignancies.\"},{\"question\":\"What evidence supports CHST as a diagnostic or prognostic marker, and what is needed next?\",\"answer\":\"The review summarizes reported diagnostic and prognostic applications, including gene mutations and unfavorable prognostic expression patterns. It also notes that larger clinical studies and robust analytical procedures are required for more reliable performance estimates.\"}]","Carbohydrate sulfotransferases - a review of emerging diagnostic and prognostic applications | PDF",1790439246,28]