[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-289640-105":59,"doc-detail-289640-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","canine-procedure-for-direct-antiglobulin-test","Canine Procedure for Direct Antiglobulin Test","","This document provides a comprehensive protocol for performing a direct antiglobulin test (DAT) on canine blood samples. It outlines the necessary materials, including wash buffer, blood collector strips, pipettes, and DAT membranes. The procedure is divided into two primary phases: the washing of red blood cells and the execution of the DAT test itself. Crucially, the guide emphasizes using fresh blood samples collected within the last 24 hours to ensure analytical reliability and diagnostic accuracy in clinical settings.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/canine-procedure-for-direct-antiglobulin-test/289640/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/canine-procedure-for-direct-antiglobulin-test/289640.png","ImageObject",300,407,{"name":92,"@type":93},"Felix Montgomery","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-22","2026-09-17",true,{"@type":102,"interactionType":103,"userInteractionCount":34},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What is the maximum age for blood samples to ensure reliable DAT results?","Question",{"text":112,"@type":113},"Blood samples should be used fresh, specifically collected within the last 24 hours, to guarantee reliable test results.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"What are the primary components required for the canine DAT procedure?",{"text":117,"@type":113},"Required materials include a wash buffer, blood collector strips, small and large pipettes, clean 1.5ml washing tubes, 5ml test tubes, and DAT membranes.",{"name":119,"@type":110,"acceptedAnswer":120},"How many times should the red blood cell washing step be repeated?",{"text":121,"@type":113},"After the initial wash, the procedure for mixing and centrifuging should be repeated two more times to prepare the cells for the DAT test.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},289640,1789636134,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":34,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":14,"language":139,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":140,"faqs":141,"seo_title":142,"seo_description":67,"update_tm":129,"read_time":24},549768064778,"https://ap-avatar.wpscdn.com/davatar_6f874abed73319feea01a86fa6f0fab8","Canine procedure for direct antiglobulin test Material provided :   \n|  |\n| --- |\n| \u003Cbr>WASH BUFFER Lot : 1246721 |\n\n1 Wash Buffer  \n10 Blood collector strips  \n10 Small pipettes  \n10 clean tubes for washing (1,5ml)  \n10 clean test tubes (5ml)  \n10 Large pipettes  \n10 DAT membranes Additional material needed : 1 centrifuge  \n ! FOR RELIABLE RESULTS: USE FRESH COLLECTED BLOOD ONLY (\u003C24 HOURS)  \nN°1: Red blood cells washing procedure  \nMix  \ngently  \n2 x  \nREPEAT STEP 2 TO 5 2 MORE TIME  \nWashed red blood cells ready for the DAT test procedure (N°2°) .  \nN°2: DAT TEST PROCEDURE  \nIn the clean test tube  \nInsert the DAT membrane.","cbCaisNx9DrPh7Kg","https://ap.wps.com/l/cbCaisNx9DrPh7Kg","pdf",940490,"English","# Red blood cells washing procedure\n## DAT test procedure","[{\"question\":\"What is the maximum age for blood samples to ensure reliable DAT results?\",\"answer\":\"Blood samples should be used fresh, specifically collected within the last 24 hours, to guarantee reliable test results.\"},{\"question\":\"What are the primary components required for the canine DAT procedure?\",\"answer\":\"Required materials include a wash buffer, blood collector strips, small and large pipettes, clean 1.5ml washing tubes, 5ml test tubes, and DAT membranes.\"},{\"question\":\"How many times should the red blood cell washing step be repeated?\",\"answer\":\"After the initial wash, the procedure for mixing and centrifuging should be repeated two more times to prepare the cells for the DAT test.\"}]","Canine Procedure for Direct Antiglobulin Test | PDF"]