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CIP2A is functionally linked to chemoresistance across cancers, but its mechanism in oxaliplatin resistance in GC remains unclear. This study evaluated CIP2A expression in human GC cells and its association with oxaliplatin resistance, using molecular and functional assays to define expression patterns, gene/protein changes, and pathway involvement.",{"@graph":69,"@context":123},[70,84,106],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/cancerous-inhibitor-of-protein-phosphatase-2a-enhances-chemoresistance-of-gastric-cancer-cells-to-oxaliplatin/378681/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/cancerous-inhibitor-of-protein-phosphatase-2a-enhances-chemoresistance-of-gastric-cancer-cells-to-oxaliplatin/378681.png","ImageObject",300,407,{"name":92,"@type":93},"moss~","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-30","2026-09-24",true,{"@type":102,"interactionType":103,"userInteractionCount":105},"InteractionCounter",{"@type":104},"ViewAction",12,{"@type":107,"mainEntity":108},"FAQPage",[109,115,119],{"name":110,"@type":111,"acceptedAnswer":112},"What is the main research focus regarding CIP2A in gastric cancer?","Question",{"text":113,"@type":114},"The study examines whether CIP2A expression is associated with and contributes to oxaliplatin resistance in human gastric cancer cells.","Answer",{"name":116,"@type":111,"acceptedAnswer":117},"How was CIP2A expression assessed and manipulated in the study?",{"text":118,"@type":114},"CIP2A expression in gastric cancer tissues and cell lines was examined by immunohistochemistry, and CIP2A was reduced in cell lines using small interfering RNA.",{"name":120,"@type":111,"acceptedAnswer":121},"What effects did CIP2A knockdown have on oxaliplatin response?",{"text":122,"@type":114},"CIP2A knockdown inhibited cell proliferation and increased sensitivity to oxaliplatin, including enhanced oxaliplatin-induced apoptosis.","https://schema.org",{"og:url":83,"og:type":125,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":127,"canonical":83},"index,follow",{"doc_id":129,"site_id":62},378681,1790633759,{"code":4,"msg":5,"data":132},{"doc_id":129,"user_id":133,"nickname":92,"user_avatar":134,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":135,"file_id":136,"file_url":137,"file_type":138,"file_size":139,"view_count":105,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":140,"language":141,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":142,"faqs":143,"seo_title":144,"seo_description":67,"update_tm":145,"read_time":146},962090895452,"https://ap-avatar.wpscdn.com/davatar_a8503ba1806abce46bf441b54a3ca4cd","| W | J | G | O | World Journal of\u003Cbr>Gastrointestinal Oncology |\n| --- | --- | --- | --- | --- |\n\nSubmit a Manuscript: [https://www.f6publishing.com](https://www.f6publishing.com) World J Gastrointest Oncol 2023 February 15; 15(2): 286-302  \nDOI: 10.4251/wjgo.v15.i2.286 ISSN 1948-5204 (online)  \nORIGINAL ARTICLE  \nBasic Study  \nCancerous inhibitor of protein phosphatase 2A enhanceschemoresistance of gastric cancer cells to oxaliplatin  \nYong-Xun Zhao, Li-Bin Ma, Ze Yang, Fang Wang, Hui-Ying Wang, Jia-Yao Dang  \nSpecialty type: Gastroenterology and hepatology  \nProvenance and peer review:  \nUnsolicited article; Externally peer reviewed.  \nPeer-review model: Single blind  \nPeer-review report’s scientific quality classification  \nGrade A (Excellent): 0  \nGrade B (Very good): B, B  \nGrade C (Good): C  \nGrade D (Fair): 0  \nGrade E (Poor): 0  \nP-Reviewer: Aurello P, Italy; Hori T, Japan; Rakić M, Croatia  \nReceived: September 8, 2022  \nPeer-review started: September 8, 2022  \nFirst decision: November 12, 2022  \nRevised: November 23, 2022  \nAccepted: January 5, 2023  \nArticle in press: January 5, 2023  \nPublished online: February 15, 2023  \nYong-Xun Zhao, Li-Bin Ma, Ze Yang, The Seventh Department of General Surgery, The First Hospital of Lanzhou University, Lanzhou 730000, Gansu Province, China  \nFang Wang, Department of Pathology, The First Hospital of Lanzhou University, Lanzhou 730000, Gansu Province, China  \nHui-Ying Wang, Jia-Yao Dang, The First Clinical Medical School, Lanzhou University, Lanzhou 730000, Gansu Province, China  \nCorresponding author: Yong-Xun Zhao, PhD, Surgeon, The Seventh Department of General Surgery, The First Hospital of Lanzhou University, No. 1 Donggang West Road, Lanzhou 730000, Gansu Province, [China.](China. yongxunzh@163.com)[ yongxunzh@163.com](China. yongxunzh@163.com)  \nAbstract  \nBACKGROUND  \nCancerous inhibitor of protein phosphatase 2A (CIP2A) is a newly discovered oncogene. It is an active cell proliferation regulatory factor that inhibits tumor apoptosis in gastric cancer (GC) cells. CIP2A is functionally related to chemoresistance in various types of tumors according to recent studies. The underlying mechanism, however, is unknown. Further, the primary treatment regimen for GC is oxaliplatin-based chemotherapy. Nonetheless, it often fails due to chemoresistance of GC cells to oxaliplatin.  \nAIM  \nThe goal of this study was to examine CIP2A expression and its association with oxaliplatin resistance in human GC cells.  \nMETHODS  \nImmunohistochemistry was used to examine CIP2A expression in GC tissues and adjacent normal tissues. CIP2A expression in GC cell lines was reduced using small interfering RNA. After confirming the silencing efficiency, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide tetrazolium and flow cytometry assays were used to evaluate cell proliferation and apoptosis caused by oxaliplatin treatment. Further, the key genes and protein changes were verified using realtime quantitative reverse transcription PCR and Western blotting, respectively, before and after intervention. For bioinformatics analysis, we used the R software and Bioconductor project. For statistical analysis, we used GraphPad Prism 6.0 and the Statistical Package for the Social Sciences software version 20.0 (IBM,  \n WJGO  [https://www.wjgnet.com](https://www.wjgnet.com) 286 February 15, 2023  Volume 15  Issue 2   \nZhao YX et al. CIP2A regulates chemoresistance of GC cells  \nArmonk, United States) .  \nRESULTS  \nA high level of CIP2A expression was associated with tumor size, T stage, lymph node metastasis, Tumor Node Metastasis stage, and a poor prognosis. Further, CIP2A expression was higher in GC cells than in normal human gastric epithelial cells. Using small interfering RNA against CIP2A, we discovered that CIP2A knockdown inhibited cell proliferation and significantly increased GC cell sensitivity to oxaliplatin. Moreover, CIP2A knockdown enhanced oxaliplatin-induced apoptosis in GC cells. He","cbCaieTdw2Xpt5UF","https://ap.wps.com/l/cbCaieTdw2Xpt5UF","pdf",9623036,18,"English","# Abstract\n## Background\n## Aim\n## Methods\n## Results\n## Conclusion\n# Core Tip\n# Citation","[{\"question\":\"What is the main research focus regarding CIP2A in gastric cancer?\",\"answer\":\"The study examines whether CIP2A expression is associated with and contributes to oxaliplatin resistance in human gastric cancer cells.\"},{\"question\":\"How was CIP2A expression assessed and manipulated in the study?\",\"answer\":\"CIP2A expression in gastric cancer tissues and cell lines was examined by immunohistochemistry, and CIP2A was reduced in cell lines using small interfering RNA.\"},{\"question\":\"What effects did CIP2A knockdown have on oxaliplatin response?\",\"answer\":\"CIP2A knockdown inhibited cell proliferation and increased sensitivity to oxaliplatin, including enhanced oxaliplatin-induced apoptosis.\"}]","Cancerous inhibitor of protein phosphatase 2A enhances chemoresistance of gastric cancer cells to oxaliplatin | PDF",1790231908,45]